Soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application
A technology of resveratrol and soybean protein, which is applied in plant protein processing, protein food ingredients, protein food processing, etc., can solve the problems of reducing the industrial value of soybean protein, and achieves safety, non-toxic side effects, and high loading rate High, good biocompatibility effect
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Embodiment 1
[0034] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:
[0035] Weigh the enzymatically hydrolyzed insoluble aggregates of soybean protein and disperse them in deionized water to prepare an insoluble aggregate dispersion with a protein concentration of 10 mg / mL. After stirring at room temperature for 2 hours, add 0.2 mg / mL resveratrol powder and mix well before using Ultrasonic breaker ultrasonic treatment 5-30min, ultrasonic power 150W, output frequency 20kHz, during the ultrasonic process use ice bath to control the sample temperature below 25 ℃. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol.
[0036] The particle size and morphology of the prepared soybean protein-based nanoparticles loaded with resveratrol were observed by transmission electron microscope (JEOL-2100). Dilute th...
Embodiment 2
[0043] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:
[0044]Weigh the insoluble aggregates of soybean protein enzymatic hydrolysis and disperse them in deionized water to prepare an insoluble aggregate dispersion with a protein concentration of 10 mg / mL. After stirring at room temperature for 2 hours, add a certain amount (0.05-1.00 mg / mL) of resveratrol The powder is stirred evenly and then ultrasonically treated with an ultrasonic breaker for 10 minutes. The ultrasonic power is 150W and the output frequency is 20kHz. During the ultrasonic process, an ice bath is used to control the sample temperature below 25°C. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol.
[0045] The average particle size, PDI and Zeta potential of the prepared resveratrol-loaded soybean protein-based nanop...
Embodiment 3
[0050] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:
[0051] Disperse the insoluble aggregates of soybean protein enzymatic hydrolysis in deionized water to prepare an insoluble aggregate dispersion liquid with a protein concentration of 1-20 mg / mL. After stirring at room temperature for 2 hours, add 0.2 mg / mL resveratrol powder and stir evenly Afterwards, ultrasonic treatment was performed for 10 minutes with an ultrasonic breaker, the ultrasonic power was 150 W, and the output frequency was 20 kHz. During the ultrasonic process, the temperature of the sample was controlled to be lower than 25° C. with an ice bath. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol. The average particle size, PDI and Zeta potential of the prepared resveratrol-loaded soybean protein-based nanoparticle...
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