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Soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application

A technology of resveratrol and soybean protein, which is applied in plant protein processing, protein food ingredients, protein food processing, etc., can solve the problems of reducing the industrial value of soybean protein, and achieves safety, non-toxic side effects, and high loading rate High, good biocompatibility effect

Active Publication Date: 2021-05-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their poor solubility in water, these components are usually discarded or used as animal feed in industrial production, and are rarely used in the food industry for human consumption, which greatly reduces the industrial value of soybean protein

Method used

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  • Soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application
  • Soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application
  • Soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:

[0035] Weigh the enzymatically hydrolyzed insoluble aggregates of soybean protein and disperse them in deionized water to prepare an insoluble aggregate dispersion with a protein concentration of 10 mg / mL. After stirring at room temperature for 2 hours, add 0.2 mg / mL resveratrol powder and mix well before using Ultrasonic breaker ultrasonic treatment 5-30min, ultrasonic power 150W, output frequency 20kHz, during the ultrasonic process use ice bath to control the sample temperature below 25 ℃. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol.

[0036] The particle size and morphology of the prepared soybean protein-based nanoparticles loaded with resveratrol were observed by transmission electron microscope (JEOL-2100). Dilute th...

Embodiment 2

[0043] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:

[0044]Weigh the insoluble aggregates of soybean protein enzymatic hydrolysis and disperse them in deionized water to prepare an insoluble aggregate dispersion with a protein concentration of 10 mg / mL. After stirring at room temperature for 2 hours, add a certain amount (0.05-1.00 mg / mL) of resveratrol The powder is stirred evenly and then ultrasonically treated with an ultrasonic breaker for 10 minutes. The ultrasonic power is 150W and the output frequency is 20kHz. During the ultrasonic process, an ice bath is used to control the sample temperature below 25°C. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol.

[0045] The average particle size, PDI and Zeta potential of the prepared resveratrol-loaded soybean protein-based nanop...

Embodiment 3

[0050] A preparation method of soybean protein-based nanoparticles loaded with resveratrol, comprising the following steps:

[0051] Disperse the insoluble aggregates of soybean protein enzymatic hydrolysis in deionized water to prepare an insoluble aggregate dispersion liquid with a protein concentration of 1-20 mg / mL. After stirring at room temperature for 2 hours, add 0.2 mg / mL resveratrol powder and stir evenly Afterwards, ultrasonic treatment was performed for 10 minutes with an ultrasonic breaker, the ultrasonic power was 150 W, and the output frequency was 20 kHz. During the ultrasonic process, the temperature of the sample was controlled to be lower than 25° C. with an ice bath. The sonicated sample was centrifuged at 10,000 g for 10 min to remove a small amount of insoluble matter to obtain soybean protein-based nanoparticles loaded with resveratrol. The average particle size, PDI and Zeta potential of the prepared resveratrol-loaded soybean protein-based nanoparticle...

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Abstract

The invention discloses a resveratrol-loaded soybean protein-based nanoparticle and its preparation method and application. The preparation method comprises the following steps: mixing soybean protein enzymatically hydrolyzed insoluble aggregates with deionized water, adding resveratrol powder , sonicate for 5‑30min, and control the temperature of the sample to be lower than 25°C during the sonication process; centrifuge the sample after sonication to remove a small amount of insoluble matter, and obtain soybean protein-based nanoparticles loaded with resveratrol. The method for preparing soybean protein-based nanoparticles loaded with resveratrol in the present invention has simple process operation, and the preparation process does not involve organic reagents such as alcohols, and is safe and has no toxic or side effects; and the prepared nanoparticles have good stability and high loading rate. high and good biocompatibility.

Description

technical field [0001] The invention relates to functional nano biological products, in particular to soybean protein-based nanoparticles loaded with resveratrol and its preparation method and application. Background technique [0002] In recent years, the application of nanotechnology in the processing of materials such as functional food, cosmetics and medicine has attracted much attention. Various nanoscale delivery systems such as nanoparticle suspensions and nanoemulsions have shown great application potential in improving the bioavailability of poorly soluble drugs and other active ingredients. [0003] At present, the food-grade raw materials commonly used to prepare nanoparticle suspensions or nanoemulsions mainly include proteins such as soybean protein isolate, whey protein isolate and β-lactoglobulin, starch nanocrystals, starch nanospheres, cellulose nanocrystals, several Tinous nanocrystals, etc. With the development of the food industry and people's increasin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/34A23J3/16A23L33/10
CPCA23J3/16A23J3/346A23L33/10
Inventor 赵谋明张远红周非白苏国万赵强忠
Owner SOUTH CHINA UNIV OF TECH
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