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Experimental method for influences of citric acid on formation of insulin resistance in mice

A technology of insulin resistance and experimental method, applied in the field of the effect of citric acid on the formation of insulin resistance in mice induced by high-fat milk, and can solve the problem of no citric acid and the like

Inactive Publication Date: 2018-06-15
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, in the prior art, there is no relevant report on the experimental method of the influence of citric acid on the formation of insulin resistance in mice

Method used

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  • Experimental method for influences of citric acid on formation of insulin resistance in mice
  • Experimental method for influences of citric acid on formation of insulin resistance in mice
  • Experimental method for influences of citric acid on formation of insulin resistance in mice

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Experimental program
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Effect test

Embodiment Construction

[0034] The technical solution of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0035] 1. Materials and methods

[0036] 1.1 Materials and reagents

[0037]

[0038]

[0039] SYBR Premix Ex Taq (Tli RNaseH Plus), Bulk Bao Biological Engineering (Dalian) Co., Ltd., RR420A;

[0040]Total RNA extraction reagent TRIzol Nanjing Zhongding Biotechnology Co., Ltd., RK01-100; AL2000 DNAMarker (100, 250, 500, 750, 1000, 2000bp) Nanjing Zhongding Biotechnology Co., Ltd., DM0101;

[0041]

[0042] All other reagents were of domestic analytical grade.

[0043] 1.2 Main instruments

[0044]

[0045]

[0046] 1.3 Preparation of reagent and test solution

[0047] Prepare CMC-Na as 0.5% CMC-Na

[0048] Both citric acid and Xuezhikang were made into the required concentration with 0.5% CMC-Na

[0049] The high-fat emulsion was prepared and used according to the literature.

[0050] 1...

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Abstract

The invention discloses an experimental method for the influences of citric acid on the formation of insulin resistance in mice. The method includes the following steps: 1, grouping and administrationof animals; 2, oral glucose tolerance test; 3, animal killing; 4, biochemical test and physiological sectioning; 5, RT-PCR; and 6, statistical analysis. Pure citric acid is studied in the invention,and blood lipids, the insulin sensitivity index, the expression of related genes and the cell morphology change of target organ tissues are detected discuss whether the citric acid can reduce or inhibit the generation of insulin resistance in the mice while simultaneously intragastrically administrating citric acid and a high-fat emulsion, so a certain basis is provided for subsequent studies on total organic acids in wild Armeniaca sibirica.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an experimental method for the influence of citric acid on the formation of insulin resistance in mice, in particular to an experimental method for the influence of citric acid on the formation of insulin resistance in mice induced by high-fat milk. Background technique [0002] Insulin resistance (Insulin resistance, IR) refers to the decreased sensitivity and responsiveness of peripheral tissues and target organs to insulin (Insulin, INS), that is, a state in which normal doses of INS produce lower than normal biological effects. Insulin resistance is not only a metabolic disease, but also the link between metabolic diseases such as hypertension, hyperlipidemia, atherosclerosis, coronary heart disease, and the basis and accompanying traits of type 2 diabetes. In recent years, the number of patients with insulin resistance has increased exponentially, and how to prevent and treat insu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00A61K31/194
CPCA61K31/194A61K49/0008
Inventor 阿依姑丽·艾合麦提戴小华王娟
Owner XINJIANG AGRI UNIV
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