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Tissue engineering artificial ligament and preparing method thereof

An artificial ligament and tissue engineering technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problem of difficult penetration of seed cells, achieve the effect of rapid recovery of activity and avoid rejection

Pending Publication Date: 2018-06-15
山东隽秀生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the tissue engineering artificial ligament provided by the present invention, living cells are evenly sown on the tissue engineering scaffold, which solves the problem that the seed cells are not easy to penetrate into the tissue engineering scaffold. Ligament structures, thereby rebuilding and repairing damaged ligaments

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Poly(lactide-caprolactone) was dissolved in a mixed solution of tetrahydrofuran and N,N-dimethylformamide to make a 0.15 g / mL polymer solution, and the solution was filled in an electrospinning The feeding port on one side of the wire equipment. Disperse the fibroblasts in the uniformly dissolved sodium alginate gel solution, fill the gel solution containing the seed cells into the feed port on the other side of the electrospinning equipment, and adjust the feed ports on both sides of the electrospinning device respectively. Propelling speed, so that the gel solution containing seed cells is in the form of a spray, evenly spread on each layer of fibers, when the thickness of the film prepared by electrospinning accumulates to about 5mm, remove it, and cut it into strips in a sterile state , the scaffold can be placed in the culture medium and cultured for 3-7 days before use as needed.

Embodiment 2

[0027] Example 2: Poly(L-lactide) was dissolved in a mixed solution of tetrahydrofuran and N,N-dimethylformamide to make a 0.05g / mL polymer solution, and the solution was filled in an electrospinning device feed port on one side. The fibroblasts were dispersed in a uniformly dissolved sodium alginate solution, and the sodium alginate gel solution containing the seed cells was added dropwise to the rapidly stirring calcium chloride solution to prepare gel microspheres containing the seed cells, which were electrospun During the preparation of tissue engineering scaffolds from silk, microspheres containing seed cells were sprayed on the scaffolds, and when the thickness of the membrane scaffolds prepared by electrospinning reached about 7mm, the membranes were removed, cut into strips under sterile conditions, and used Before that, the scaffold can be placed in cell culture medium and cultivated for 3-7 days.

Embodiment 3

[0028] Example 3: Poly(L-lactide) was dissolved in a mixed solution of tetrahydrofuran and N,N-dimethylformamide to make a 0.3g / mL polymer solution, and the solution was filled in an electrospinning device feed port on one side. Disperse the tenocytes and fibroblasts in the uniformly dissolved extracellular matrix solution, fill the gel solution containing the seed cells in the inlet of the other side of the electrospinning equipment, and adjust the inlets on both sides of the electrospinning respectively The propulsion speed is so that the gel solution containing the seed cells is sprayed and evenly spread on each layer of fibers. When the thickness of the membrane prepared by electrospinning is about 5mm, it is removed and cut into strips under sterile conditions. If necessary, the scaffold can be placed in the culture medium and cultured for 3-7 days before use.

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PUM

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Abstract

The invention discloses a tissue engineering artificial ligament. The tissue engineering artificial ligament is composed of a degradable macromolecule material support and seed cells pre-encapsulatedby gel. The invention further discloses a preparing method of the tissue engineering artificial ligament. By means of the seed cells pre-encapsulated by the gel, when the degradable macromolecule support is prepared through electrostatic spinning, the seed cells are uniformly sowed on the support, so that the seed cells are organically combined with the support, and the artificial ligament which contains living cells and has the function of reconstructing the ligament tissue is formed.

Description

technical field [0001] The invention relates to an artificial ligament in the field of biomedical technology, in particular to an active artificial ligament used for repairing and reconstructing damaged ligaments of a human body and a preparation method thereof. Background technique [0002] Ligaments are cords of connective tissue that connect bones to each other. They are closely paralleled with elastic fibers. They attach to the movable parts of the bones, but limit their range of motion to avoid damage, thereby maintaining the stability of the joint. In daily life, ligament injuries of ordinary people are common in ankle joints, knee joints, palm joints, and finger joints. Ligament ruptures are generally caused by rapid movements beyond the range of joint motion during strenuous activities, resulting in passive stretching of related ligaments. Torn or completely broken. The knee ligaments are the most vulnerable to injury during sports, especially the cruciate ligaments...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/20A61L27/36A61L27/38A61L27/50A61L27/58
CPCA61L27/18A61L27/20A61L27/3633A61L27/3804A61L27/3826A61L27/50A61L27/58A61L2430/10C08L67/04C08L5/04
Inventor 姜红任孝敏李利明
Owner 山东隽秀生物科技股份有限公司
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