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Preparation process for extracting scopolamine from belladonna

A technology of scopolamine and belladonna, which is applied in the field of enrichment and purification of scopolamine by using macroporous adsorption resin, can solve the problems of low extraction purity and low extraction rate, and achieve the effects of high extraction purity, fast adsorption and rich source of raw materials.

Inactive Publication Date: 2018-06-15
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a method for enriching and purifying belladonna extract using macroporous adsorption resins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: a kind of method utilizing macroporous adsorption resin to enrich and purify hyoscyamine in belladonna, its steps are as follows:

[0022] (1) crush the belladonna and pass through a 60-mesh sieve, use hydrochloric acid water with pH=4 at the ratio of material:water=1:12 to heat and reflux to extract twice, each time for 2 hours, and combine the extracts;

[0023] (2) filtering the belladonna extract treated in (1), and drying under reduced pressure to obtain an extract-like primary extract;

[0024] (3) the obtained hyoscyamine crude extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is a D4006 type resin;

[0025] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol to carry out gradient elution in sequence in the macroporous adsorption resin layer column, track and detect with thin-layer chromatography, and collect hyoscyamine at each stage eluent;

[0026] (5) concentrating and reclaiming ethanol from ...

Embodiment example 2

[0029] Implementation case 2: a method utilizing macroporous adsorption resin to enrich and purify hyoscyamine in belladonna, the steps are as follows:

[0030] (1) crush the belladonna and pass through a 40-mesh sieve, use hydrochloric acid water with pH=6 at the ratio of material:water=1:10 to heat and reflux to extract twice, each time for 2 hours, and combine the extracts;

[0031] (2) filtering the belladonna extract treated in (1), and drying under reduced pressure to obtain an extract-like primary extract;

[0032] (3) the obtained hyoscyamine crude extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is LX-100 type resin;

[0033] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol to carry out gradient elution in sequence in the macroporous adsorption resin layer column, track and detect with thin-layer chromatography, and collect hyoscyamine at each stage eluent;

[0034] (5) concentrating and reclaiming ethanol from ...

Embodiment example 3

[0037] Implementation case 3: a method for enriching and purifying hyoscyamine in belladonna using macroporous adsorption resin, the steps are as follows:

[0038] (1) crush the belladonna and pass through an 80-mesh sieve, use hydrochloric acid water with pH=5 at the ratio of material:water=1:12 to heat and reflux to extract twice, each time for 2 hours, and combine the extracts;

[0039] (2) filtering the belladonna extract treated in (1), and drying under reduced pressure to obtain an extract-like primary extract;

[0040] (3) the obtained hyoscyamine crude extract is adsorbed on a macroporous adsorption resin chromatography column, and the macroporous resin used is AB-8 type resin;

[0041] (4) Use 70%, 75%, 80%, 85%, 90%, 95% ethanol to carry out gradient elution in sequence in the macroporous adsorption resin layer column, track and detect with thin-layer chromatography, and collect hyoscyamine at each stage eluent;

[0042] (5) concentrating and reclaiming ethanol fro...

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Abstract

The invention adopts the macroporous adsorption technology to separate and purify scopolamine, has the advantages of good adsorption selectivity to the scopolamine, fast adsorption and desorption, large adsorption capacity, convenient and rapid extraction, abundant raw material sources, low production cost, obvious separation effect, and high extraction purity, and overcomes the shortcomings of relatively low conventional extraction rate and low extraction purity. Macroporous adsorptive resin with stable physical and chemical properties, large surface area, fast exchange rate, high mechanicalstrength, strong anti-pollution ability and good thermal stability is selected, and the macroporous adsorptive resin is insoluble in acids, alkalis and organic enzymes, has good selectivity for organic substances, is not affected by the presence of inorganic salts and strong ionic low molecular compounds, can selectively adsorb the scopolamine from a solution by physical adsorption, and is fast inadsorption and desorption and large in adsorption capacity.

Description

Technical field: [0001] The invention belongs to the technical field of traditional Chinese medicines, and relates to a new method for extracting hyoscyamine from belladonna, in particular to a new method for enriching and purifying hyoscyamine by using a macroporous adsorption resin. Background technique: [0002] Belladonna (scientific name: Atropa belladonna) is a herbaceous plant of the family Solanaceae. The perennial herb native to Western Europe was later transplanted to North Africa, West Asia, North America and other places, and it was also introduced and cultivated in China. Its native place is the shady and humid area of ​​the mountain, and it grows in groups in the calcareous soil. It germinates in early spring, with a total length of 40-50 cm and a maximum height of 5 meters. The root is cylindrical, 5-15 mm in diameter, light grayish brown on the surface, with longitudinal wrinkles; the old root is woody, the fine root is easy to break, the cross section is f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D451/10
CPCC07D451/10
Inventor 姚德坤王雷李玉伟苏学辉万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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