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A kind of rna grinding extraction method

An extraction method and a grinding method technology, applied in the field of RNA extraction, can solve the problems of inaccurate, sore hands and manual grinding, and achieve the effects of avoiding frostbite, improving grinding efficiency and eliminating fatigue.

Active Publication Date: 2019-08-16
山东卫康医学检验有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to make up for the deficiencies in the prior art, the present invention proposes an RNA grinding and extraction method, which is mainly used to solve the problems of hand soreness and imprecise manual grinding during traditional RNA extraction.

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  • A kind of rna grinding extraction method
  • A kind of rna grinding extraction method
  • A kind of rna grinding extraction method

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Embodiment Construction

[0033] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0034] according to Figure 1-6 As shown, an RNA grinding and extraction method, the RNA extraction and grinding method adopts the following RNA grinding equipment, the RNA grinding equipment includes a box body 1, a centrifuge tube 11, a grinding head 12, a power module 2, a clamping and fixing module 3, and a heat preservation module 4. Nitrogen circulation pressure maintaining module 5, nitrogen introduction module 6 and pressing module 7, the box body 1 is provided with a cantilever 13 that can be adjusted up and down; the power module 2 is fixed on the cantilever 13; the grinding head 12 and The power module 2 is connected, and the power module 2 drives the grinding head 12 to rotate forward and backward and move up and down in a straight lin...

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Abstract

The invention belongs to the technical field of RNA extraction, and particularly relates to a grinding extraction method for RNA. An RNA grinding device is adopted for the grinding extraction method for RNA and comprises a box. A cantilever is arranged on the box. A power module is fixed to the cantilever. A grinding head is connected with the power module, and the power module drives the grindinghead to rotate forwards or reversely and to linearly move upwards and downwards. A clamping and fixing module is located on the box and used for clamping and fixing a centrifugal tube on the box. A heat preservation module is used for heat preservation of the centrifugal tube. A nitrogen circulation and pressure maintenance module is used for introducing liquid nitrogen into the box and keeping the nitrogen pressure in the box stable. A nitrogen guide-in module communicates with the box and is used for guiding nitrogen into the grinding head. According to the grinding extraction method for RNA, grinding extraction of RNA is achieved mechanically, grinding is sufficient and refined, and thus RNA extraction is facilitated.

Description

technical field [0001] The invention belongs to the technical field of RNA extraction, in particular to an RNA grinding and extraction method. Background technique [0002] RNA in cells can be divided into three categories: messenger RNA, transfer RNA, and ribosomal RNA. Total RNA with high purity and good integrity is a necessary prerequisite for gene cloning, gene expression, and gene function analysis. These three types of RNA All exist in the cytoplasm, so the essence of total RNA extraction from different tissues is to crack cells, release RNA, and remove impurities such as protein and DNA in different ways, and finally obtain high-purity RNA products. [0003] At present, the commonly used total RNA extraction technology in the laboratory is Trizol extraction after low-temperature homogenization. Low-temperature homogenization generally adopts the liquid nitrogen grinding method, and the RNA needs to be fully ground in the process of extracting RNA by the liquid nitro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B02C19/08B02C19/18G01N1/28
CPCB02C19/08B02C19/186G01N1/28
Inventor 尹康康张立龙程华
Owner 山东卫康医学检验有限公司
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