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Test paper strip for detecting glycyrrhizic acid, preparation method and applications thereof

A technology of glycyrrhizic acid and test strips, which is applied in biological tests, measuring devices, material testing products, etc., can solve the problems of low antibody titer and affect the recognition of the body's immune system, and achieves good specificity, low detection cost, and sensitivity. high effect

Active Publication Date: 2018-06-22
河北省中药材质量检验检测研究中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the characteristic structure of glycyrrhizic acid is easily interfered by the local microchemical environment or steric hindrance of the carrier protein, which affects the recognition of the body's immune system, making it difficult to produce specific antibodies or the antibody titers produced are low

Method used

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  • Test paper strip for detecting glycyrrhizic acid, preparation method and applications thereof
  • Test paper strip for detecting glycyrrhizic acid, preparation method and applications thereof
  • Test paper strip for detecting glycyrrhizic acid, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1 Preparation of test strips for detecting glycyrrhizic acid

[0098] The preparation method of this test strip mainly comprises the following steps:

[0099]1) Prepare a conjugate release pad sprayed with glycyrrhizic acid monoclonal antibody-colloidal gold complex;

[0100] 2) preparing a reaction membrane with a detection area coated with a glycyrrhizic acid hapten-carrier protein conjugate and a quality control area coated with a goat anti-mouse anti-antibody;

[0101] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC substrate into a test strip.

[0102] The following step-by-step detailed description:

[0103] 1. Synthesis of glycyrrhizic acid hapten (synthetic route sees attached image 3 ) and identification

[0104] Take 0.5g of glycyrrhizic acid, add ethanol to dissolve, add 0.1g of sodium bicarbonate, add 0.15g of 1-(4-amino-phenyl)carbodihydrazide, h...

Embodiment 2

[0138] Example 2 Application of test strips for detecting glycyrrhizic acid

[0139] 1. Sample pretreatment

[0140] Take 0.1g of licorice medicinal material, decoction pieces or roasted licorice powder, add 25mL of 70% ethanol to the sample cup, 40Hz ultrasonic treatment for 30min, shake well, filter and take the filtrate to obtain the sample concentrate;

[0141] Take 900 μL of the concentrated solution of the decoction pieces sample, add it to 3600 μL 0.01mol / L PBS, and mix well to obtain the sample of licorice decoction pieces to be tested;

[0142] Take 900 μL of the medicinal material sample concentrate, add it to 4100 μL 0.01mol / L PBS, and mix well to obtain the test sample of the licorice medicinal material;

[0143] Take 900 μL of concentrated licorice sample, add it to 1600 μL 0.01mol / L PBS, and mix well to obtain the sample of licorice decoction pieces to be tested.

[0144] 2. Test with test strips

[0145] Take out the test strip, place it on a horizontal table, ...

Embodiment 3

[0150] Embodiment 3 Detection of the performance of the test strip for detecting glycyrrhizic acid

[0151] 1. Detection limit test

[0152] ① Sensitivity of detection reference substance

[0153] Weigh 1 mg of glycyrrhizic acid and dissolve it in 1 mL of methanol, and dilute it with 0.01 mol / L PBS. When the standard product is diluted to 2 mg / L, the T line position is wireless; when the standard product is diluted to 1 mg / L, the T line can be seen Appeared; when the standard was diluted to 0.5mg / L, the T line was clearly visible. The test strip can disappear at 2mg / L, so its sensitivity is 2mg / L.

[0154] ② Sensitivity of detecting licorice samples

[0155] Properly dilute the test solution of the Chinese medicine licorice sample with 0.01mol / L PBS. When the sample is diluted to 7.2mg / L, the T line position is wireless; when the standard product is diluted to 3.6mg / L, the T line can be seen; when the standard product When diluted to 1.8mg / L, the T line is clearly visible....

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Abstract

The invention provides a test paper strip for detecting glycyrrhizic acid, a preparation method and applications thereof. The test paper strip comprises a sample absorption pad, a conjugate release pad, a reaction membrane, a water absorption pad and a base plate, wherein the reaction membrane has a glycyrrhizic acid hapten-carrier protein conjugate coated detection zone and a goat anti-mouse anti-antibody coated quality control zone, and the conjugate release pad is sprayed with a glycyrrhizic acid monoclonal antibody-colloidal gold complex. The present invention further provides a method fordetecting glycyrrhizic acid in a sample by using the test paper strip. According to the present invention, the test paper strip and the detection method have characteristics of high specificity, highsensitivity, simple operation, quick result reading, low cost and the like, and are suitable for the screening of a large number of samples and the on-site quality monitoring of traditional Chinese medicines.

Description

technical field [0001] The invention relates to a detection test strip, in particular to an immune colloidal gold test strip for detecting glycyrrhizic acid, which is especially suitable for the content of glycyrrhizic acid in Chinese medicinal materials, decoction pieces, Chinese patent medicines, health care products, food, cosmetics, etc. detection. Background technique [0002] Modern studies have confirmed that glycyrrhizic acid (Glycyrrhizic acid, GA, C 42 h 62 o 16 ) is the main bioactive substance in the traditional Chinese medicine licorice. It contains two molecules of glucuronic acid and one molecule of glycyrrhetinic acid. The molecular weight is about 822 Da, and its content is 3% to 13%. , immune regulation, anti-virus, anti-cancer, liver protection and stable cell membrane. Licorice and its preparations often use glycyrrhizic acid as a quantitative indicator to evaluate the quality of medicinal materials and finished medicines, the stability and pros and c...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/531
CPCG01N33/531G01N33/558G01N33/577G01N33/94Y02A50/30
Inventor 冯红张兰兰李细芬凃媛
Owner 河北省中药材质量检验检测研究中心有限公司
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