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Preparation method of copper ion chelating peptide as green feed additive

A green feed, copper ion technology, applied in the preparation method of peptides, hybrid peptides, animal feed and other directions, can solve the problems of difficulty in keeping anchovies fresh and selling, and the resources of anchovy have not been fully developed, avoiding heavy metal pollution, The effect of improving copper chelation rate and avoiding waste

Inactive Publication Date: 2018-06-29
连云港大海饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fishing season of anchovy is strong. During the fishing season, the daily catch of a single boat can reach 40 tons, which brings great difficulties to the preservation and sale of anchovy
Anchovy resources have been underexploited for a long time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, a kind of preparation method of green feed additive copper ion chelating peptide, comprises the following steps:

[0030] (1) Preparation of anchovy polypeptide dry powder: thaw the frozen anchovy with the required weight in the pre-cooling room to -5~0℃, then wash it with clean water, put it into a chopping machine and cut it into minced meat, and chop it for 10 minutes to ensure Cut and mix evenly without large pieces of tissue; disperse the minced meat in distilled water to form fish paste, the mass ratio of minced meat to distilled water is 1:3; before enzymolysis, put 0.3% of the mass of fish paste into a small amount of trypsin at 40 Fully dissolve in warm water and set aside, then heat the fish paste to a temperature of 40°C. In order to ensure more complete enzymatic hydrolysis, put the fish paste into a digital constant temperature water bath oscillator, then add phosphate buffer to adjust the pH value to 6.5, and then Raise the temperature to 50°...

Embodiment 2

[0036] In Example 2, the pH value of the fish paste in step (1) in Example 1 was adjusted to 7.0, and the molecular weight of the protein and peptide in the peptide solution prepared from the obtained peptide dry powder was all distributed below 1000 Da through the detection of the G15 chromatographic column. , the protein peptides with a molecular weight of 0-200Da accounted for 30.34%, the protein peptides with a molecular weight of 200-500Da accounted for 57.75%, and the protein peptides with a molecular weight of 500-1000Da accounted for 12.17%. It can be seen that the molecular weight of less than 500Da accounted for 88.09% .

Embodiment 3

[0037] In Example 3, the pH value of the fish paste in step (1) in Example 1 was adjusted to 7.5, and the molecular weight distribution of the protein peptides in the peptide liquid prepared from the obtained peptide dry powder was all below 1000 Da through the detection of the G15 chromatographic column. , the protein peptides with a molecular weight of 0-200Da accounted for 38.69%, the protein peptides with a molecular weight of 200-500Da accounted for 48.53%, and the protein peptides with a molecular weight of 500-1000Da accounted for 12.78%. It can be seen that the molecular weight of less than 500Da accounted for 87.22%. .

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Abstract

The invention provides a preparation method of copper ion chelating peptide as a green feed additive. The preparation method comprises steps as follows: (1), preparation of dry anchovy polypeptide powder: frozen anchovy is unfrozen and then is cleaned with clear water and uniformly chopped into meat paste, the meat paste is dispersed in distilled water, fish pulp is formed, PH value of the fish pulp is regulated to 6.5-7.5, exogenous enzyme is added for enzymolysis, enzymatic hydrolysate is formed, enzyme in the enzymatic hydrolysate is inactivated, then the enzymatic hydrolysate is subjectedto centrifugal treatment, a supernatant is taken, concentrated and dried, and the dry polypeptide powder is obtained; (2), preparation of copper ion chelating peptide: a peptide liquid is prepared from the dry polypeptide powder, PH value of the peptide liquid is regulated to 6.8-7.5, a soluble copper solution is added to the peptide liquid for a chelation reaction, centrifugal treatment is performed after the chelation reaction, precipitate is concentrated and dried, and the copper ion chelating peptide is obtained. According to the preparation method, the ratio of protein peptide with molecular weight distribution being 500 Da or lower in the peptide liquid is higher than 85%, therefore, the prepared copper ion chelating peptide is absorbed better by animals, and copper absorption rate is effectively increased.

Description

technical field [0001] The invention relates to a preparation method of a feed additive, in particular to a preparation method of a green feed additive copper ion chelating peptide. Background technique [0002] At present, most of the organic chelated copper used as an aquatic feed additive is chelated copper with compound amino acids, and its intramolecular charge tends to be neutral, and as a reversible oxygen carrier, it can reversibly combine and release molecular oxygen, plus In the pH environment of aquatic animals, its solubility is good, it is easily absorbed by the intestinal mucosa, and the absorption rate is significantly improved. For example, feeding shrimp with amino acid chelated copper can increase its absorption by 3 times compared with inorganic copper (copper sulfate). Amino acid chelated copper is also closer to the natural form of enzymes, which can enhance the activity of enzymes in aquatic animals, thereby improving the utilization of protein, fat and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/107C12P21/06A23K20/147
CPCA23K20/147C12P21/06C07K19/00
Inventor 陈忆凤朱勤颜婕
Owner 连云港大海饲料有限公司
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