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Gene sequencing substrate, preparation method thereof and gene sequencing device

A gene sequencing and substrate technology, applied in biochemical equipment and methods, chemical instruments and methods, optomechanical equipment, etc., can solve the problems of high production costs, reduce production costs, fast detection response, and facilitate high-throughput testing Effect

Active Publication Date: 2018-06-29
BOE TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the problem of high production cost of the existing high-throughput sequencing chip, the present invention provides a gene sequencing substrate and its preparation method, and a gene sequencing device

Method used

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  • Gene sequencing substrate, preparation method thereof and gene sequencing device
  • Gene sequencing substrate, preparation method thereof and gene sequencing device
  • Gene sequencing substrate, preparation method thereof and gene sequencing device

Examples

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Embodiment 1

[0031] This embodiment provides a gene sequencing substrate, such as figure 2 As shown, a substrate 51 formed of plastic material is included, and a concave structure 52 is provided on one side of the substrate 51, and the concave structure 52 is a reaction pool for gene sequencing; on the inner wall of the concave structure 52 A first protective layer 53 is provided.

[0032] The gene sequencing substrate of this embodiment uses a plastic material as a substrate 51, and a concave structure 52 is formed on the plastic surface as a reaction pool (also called a nanowell). Among them, the plastic material refers to the material that is not destroyed even though it undergoes significant deformation under the action of external force. Since the substrate 51 has plasticity, a simple process can be used to form the concave structure 52, which reduces the production cost of the gene sequencing substrate. At the same time, the function of adding the first protective layer 53 on the ...

Embodiment 2

[0034] This embodiment provides a gene sequencing substrate, such as image 3 , Figure 4 As shown, a substrate 51 formed of plastic material is included, and a concave structure 52 is provided on one side of the substrate 51, and the concave structure 52 is a reaction pool for gene sequencing; on the inner wall of the concave structure 52 A first protective layer 53 is provided. Wherein, the first protective layer 53 can be as figure 2 Shown is the full protective layer, which can also be shown as image 3 As shown in the section of , it is only provided at the position for contacting with the reaction liquid to save material.

[0035] As an optional implementation in this embodiment, the plastic material includes polymethyl methacrylate (PMMA), polydimethylsiloxane (PDMS), polyethylene terephthalate (PET ) in any one or several.

[0036] That is to say, unlike the rigid glass substrate 51 in the prior art, a plastic polymer material is selected as the substrate 51 in t...

Embodiment 3

[0044] This embodiment provides a method for preparing a gene sequencing substrate, such as Figure 7 As shown, the following preparation steps are included:

[0045] S01, heating the plastic material to soften the plastic material; wherein, the actual heating temperature in this step can refer to the glass transition temperature of the material. The plastic material is selected from any one or more of polymethylmethacrylate (PMMA), polydimethylsiloxane (PDMS), and polyethylene terephthalate (PET). The effect of the heating in this step is to soften the plastic material so as to deform its surface to form the concave structure 52 . The specific heating temperature can be changed according to the selection of different materials.

[0046] S02, using a nanoimprint process to directly form a concave structure 52 on one side of the plastic material, and the concave structure 52 is a reaction pool for gene sequencing;

[0047]Nano-imprinting is to use a rigid stamper with nano-p...

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Abstract

The invention provides a gene sequencing substrate, a preparation method thereof and a gene sequencing device, belongs to the technical field of gene sequencing and solves the problem of high manufacturing cost of an existing high-flux sequencing chip. According to the gene sequencing substrate, a plastic material is taken as a substrate body, and an indent structure is formed on a plastic surfaceto serve as a reaction tank. The substrate body has plasticity, so that the indent structure can be formed with a simple technology, and production cost of the gene sequencing substrate is reduced. Meanwhile, a first protecting layer additionally arranged on the inner wall of the indent structure has functions as follows: the inner wall of the indent structure prepared from the plastic material is protected and prevented from being corroded by a reaction liquid. According to the gene sequencing device, the reaction tank, a micro-channel, an injection port and a sample outlet are integrated onto a first substrate and a second substrate for liquid detection, integration level of the device is quite high, quite few test samples and reaction reagents are required, the detection reaction speedis high, and high-flux testing is facilitated.

Description

technical field [0001] The invention belongs to the technical field of gene sequencing, and in particular relates to a gene sequencing substrate, a preparation method thereof, and a gene sequencing device. Background technique [0002] Gene sequencing technology is the most commonly used technology in modern molecular biology research. Since the development of the first generation of gene sequencing in 1977, gene sequencing technology has achieved considerable development. Among them, the development process of gene sequencing technology includes: the first-generation sanger sequencing technology, the second-generation high-throughput sequencing technology, the third-generation single-molecule sequencing technology, and the fourth-generation nanopore sequencing technology; the current mainstream sequencing technology in the market is still based on Second-generation high-throughput sequencing. [0003] The second-generation high-throughput sequencing technologies mainly inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6869
CPCC12Q1/6869C12Q2535/122B01J2219/00317B01L3/502707B01L2300/0877B01L2300/0851B01L2300/0896G03F7/0002
Inventor 蔡佩芝庞凤春耿越古乐车春城
Owner BOE TECH GRP CO LTD
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