Spiral variable-section microfluidic pcr chip and manufacturing method thereof
A variable cross-section, spiral technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve problems such as products not on the market, no technical parameters reported, etc.
Active Publication Date: 2021-08-20
NAT UNIV OF DEFENSE TECH
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In addition, Shanghai Yimu Company has also developed a MF-100 microfluidic PCR instrument, but this product has not been listed, and there is no relevant technical parameter report
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[0034] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. The components of the embodiments of the invention generally described and illustrated in the figures herein may be arranged and designed in a variety of different configurations. Accordingly, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts shall fall within the protection scope of the present invention.
[0035] In the description of the pres...
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Abstract
The invention provides a spiral variable cross-section microfluidic PCR chip and a manufacturing method thereof. The microfluidic PCR chip includes a reaction system and a temperature control system; the reaction system includes a bonded substrate and a cover sheet, and the The substrate is provided with a substrate flow channel, and the substrate flow channel includes a constant cross-section flow channel and a spiral variable cross-section flow channel connected together. The spiral variable cross-section flow channel is spirally distributed with the center of the substrate as the center of the circle. The cross-sectional size of the spiral variable cross-section flow channel decreases gradually with the increase of the flow channel length. The invention is small in structure size and light in weight, so that many auxiliary equipment for PCR reactions can be highly integrated. The invention can divide the substrate into three temperature zones. The temperature control system also adopts the constant temperature independent control of the three temperature zones, and improves the uniformity control of the temperature zone through the heat conduction element, which can reduce the impact of PCR reaction on the temperature control system and control the temperature at a high speed and accurately. Elevation requirements.
Description
technical field [0001] The invention relates to the technical field of polymerase chain (PCR) amplification reaction, in particular to a spiral variable cross-section microfluidic PCR chip and a manufacturing method thereof. Background technique [0002] Polymerase chain reaction (PCR) is a technique for selectively amplifying DNA or RNA sequences in vitro. The basic reaction principle is a process of repeated DNA template unzipping, primer and template DNA binding, and DNA polymerase catalyzing the formation of new DNA strands. Repeating this process continuously can make the target DNA fragment exponentially amplify. The essence of the PCR amplification reaction is the reaction of the reaction solution in three different temperature zones, including denaturation (dsDNA denatures into ssDNA at 92-96°C), annealing (primers bind to the complementary region of the template at 45-72°C), extension (in the At 72°C, TaqDNA polymerase catalyzes the DNA template-primer conjugate an...
Claims
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IPC IPC(8): C12M1/38C12M1/00
CPCB01L3/502792B01L7/52
Inventor 董培涛吴学忠何昱王朝光张晨煜陈剑
Owner NAT UNIV OF DEFENSE TECH