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Method for producing single cell protein by use of methane

A single-cell protein and production method technology, which is applied in the field of bacterial strains and single-cell protein production, can solve the problems of high fishmeal prices, and achieve the effects of being suitable for industrial production, environmentally friendly, energy-saving, and highly adaptable

Pending Publication Date: 2018-07-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, due to the continuous reduction of fishing quotas by the main exporting countries of fishmeal, the decline of fishery resources caused by climate change and environmental deterioration, and the implementation of fishing bans in countries such as Peru, the price of fishmeal has also continued to rise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Single-cell protein production using Methylococcus capsulatus ATCC33009

[0028] Wherein, the culture medium formula used:

[0029] Inorganic salt liquid medium: solvent is water, solute is 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 •7H 2 O, 1.0g NaCl and 1.0g (NH 4 ) 2 SO 4 , pH natural.

[0030] (1) Primary seed culture: Inoculate the Methylococcus capsulatus ATCC33009 strain preserved in glycerol tubes on the ultra-clean workbench (instrument model SW-CJ-1FD) to the seed slant medium (test tube size 180mm×20mm ), put filter-sterilized methane gas into it, and after culturing at 30°C for 96 hours, take 1 cultivated slant seed, wash all the slant seeds with 10mL sterile water, and inoculate into 2.5% paraffin oil, 250mL inorganic Into a 2L Erlenmeyer flask with saline liquid medium, pass through filter-sterilized methane gas, place it in a shaker at 30°C and 220rpm, and cultivate until OD 600 =9-10, the microscopic examination of the seed cells is robust...

Embodiment 2

[0039] Application of Methylococcus capsulatus ATCC33009, Brevibacillus soil Brevibacillus agri ACCC10247 and Bacillus aerothiamine thermophiles Aneurinibacillus thermoaerophilus Single-cell protein production by ACCC10261 mixed fermentation

[0040] Wherein, the culture medium formula used:

[0041] Inorganic salt liquid medium: solvent is water, solute is 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 , 0.2g MgSO 4 •7H 2 O, 1.0g NaCl and 1.0g (NH 4 ) 2 SO 4 , pH natural.

[0042] (1) Primary seed culture: Methylococcus capsulatus ATCC33009 and Brevibacillus agroides preserved in glycerol tubes on an ultra-clean bench (instrument model SW-CJ-1FD) Brevibacillus agri ACCC10247 and Bacillus aerothiamine thermophiles Aneurinibacillus thermoaerophilus Inoculate ACCC10261 strains on the seed slant medium (test tube size 180mm×20mm), pass through filter-sterilized methane gas, cultivate at 30°C for 96 hours, take the cultured slant seeds, and use 10mL sterile water to inoculate ...

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Abstract

The invention relates to a method for industrially producing a single cell protein by use of methane, and provides a new method for effectively solving the shortage of feed proteins of livestock and poultry and aquatic animals in China and solving the problems such as fast rising in production cost of aquaculture. According to the method, the single cell protein is prepared from one or more strains of Methylococcus capsulatus ATCC33009, Brevibacillus agri ACCC10247, and Aneurinibacillus thermoaerophilus ACCC10261 by use of the methane. The crude protein content of the produced single cell protein is over 70%, the fermenting strain has strong adaptability to fermentation conditions, and produced feeds are safe and non-toxic.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a bacterial strain for producing single-cell protein by transforming methane and a method for producing single-cell protein. Specifically, it refers to the use of methane resources to form a new methane downstream product production chain, convert methane into single-cell protein products, and solve the problem of rising animal protein feed prices in recent years, increasing costs in the breeding industry, and traditional single-cell protein production is limited by raw material sources. It can not be produced on a large scale, and has the advantages of green environmental protection, low production energy consumption, not affected by the environment and climate, continuous production, and easy control. It is a new alternative source of traditional protein feed. Background technique [0002] With the rapid development of animal husbandry, the shortage of animal feed resources has attra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 储卫华周淑鑫
Owner CHINA PHARM UNIV
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