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Small-molecular compound/combination for preventing, delaying or reversing aging of cells, tissues, organs and bodies, product thereof, and use of compound/combination and product

A technology of small molecule compounds and cells, applied in the field of cell biology, can solve the problems of lack of screening and low feasibility.

Active Publication Date: 2018-07-20
云南美颜美龄生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports and studies on small molecular compounds or small molecular compound combinations for anti-aging. On the one hand, due to the lack of methods and systems for screening such small molecular compounds; , due to about 25% genetic differences between humans and mice, it is not feasible to apply to the same kind of human cells

Method used

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  • Small-molecular compound/combination for preventing, delaying or reversing aging of cells, tissues, organs and bodies, product thereof, and use of compound/combination and product
  • Small-molecular compound/combination for preventing, delaying or reversing aging of cells, tissues, organs and bodies, product thereof, and use of compound/combination and product
  • Small-molecular compound/combination for preventing, delaying or reversing aging of cells, tissues, organs and bodies, product thereof, and use of compound/combination and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1. Isolation of skin fibroblasts

[0060] 1.1 Obtain skin tissue pieces with a diameter of about 1 cm from volunteers, and separate skin fibroblasts by the adherent method. The isolated cells are cultured in the basal culture medium, which is: 10% fetal bovine serum (Hyclone)+100U / ml penicillin (Sigma) + 100 μg / ml streptomycin (Sigma) + high glucose DMDM.

[0061] 1.2 Massive expansion of cell passage, cell generation In this experiment, the cells are the 12th generation, the day before the treatment (Day-1), the inoculated cell density is 1-2.5×10 4 / cm 2 Cultured at 37°C, 5% CO 2 in the incubator.

[0062] 2. Small molecule compound treatment of skin fibroblasts

[0063] Replace the basic culture medium with a small molecule treatment solution, and culture the cells for 2-20 days. The small molecule treatment solution refers to: 10% fetal bovine serum (Hyclone) + 100U / ml penicillin (Sigma) + 100 μg / ml streptomycin (Sigma) +High glucose DMDM ​​medium (Gibco)+fors...

Embodiment 2

[0084] 1. Culture of bone marrow mesenchymal stem cells

[0085] 1.1 Purchase commercially available human-derived bone marrow mesenchymal stem cells or isolate mesenchymal stem cells from the bone marrow of volunteer bone fragments after bone trauma, and culture the cells in the basal culture medium, the basal culture medium: 8% fetal bovine serum (Hyclone)+100 U / ml penicillin (Sigma)+100 μg / ml streptomycin (Sigma)+low sugar DMDM.

[0086] 1.2 Massive expansion of cell passage, cell generation In this experiment, the cells are the 10th passage, the day before the treatment (Day-1), the inoculated cell density is 1-2.5×10 4 / cm 2 Cultured at 37°C, 5% CO 2 in the incubator.

[0087] 2. Small molecule compound treatment of bone marrow mesenchymal stem cells

[0088] Replace the basic culture medium with a small molecule treatment solution, and culture the cells for 2-20 days. The small molecule treatment solution refers to: 10% fetal bovine serum (Hyclone) + 100U / ml penicill...

Embodiment 3

[0109] 1. Isolation of skin fibroblasts

[0110] 1.1 Obtain skin tissue pieces with a diameter of about 1 cm from volunteers, and separate skin fibroblasts by the adherent method. The isolated cells are cultured in the basal culture medium, which is: 10% fetal bovine serum (Hyclone)+100U / ml penicillin (Sigma) + 100 μg / ml streptomycin (Sigma) + high glucose DMDM.

[0111] 1.2 Massive expansion of cell passage, cell generation In this experiment, the cells are the 12th generation, the day before the treatment (Day-1), the inoculated cell density is 1-2.5×10 4 / cm 2 Cultured at 37°C, 5% CO 2 in the incubator.

[0112] 2. Small molecule compound treatment of skin fibroblasts

[0113]Replace the basic culture medium with a small molecule treatment solution, and culture the cells for 2-20 days. The small molecule treatment solution refers to: 10% fetal bovine serum (Hyclone) + 100U / ml penicillin (Sigma) + 100 μg / ml streptomycin (Sigma) +High glucose DMDM ​​medium (Gibco)+forsk...

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Abstract

The invention discloses a small-molecular compound / combination for preventing, delaying or reversing the aging of cells, tissues, organs and bodies, a product thereof, and a use of the compound / combination and the product. The small-molecular compound / combination includes at least one of a DNMT inhibitor, an HMT inhibitor, a histone demethylase inhibitor, a TGF-beta inhibitor, a WNT / beta-catenin agonist, a cAMP agonist, a lysine deacetylase inhibitor, an RAR agonist and an ROCK inhibitor. The small-molecular compound or the combination thereof is adopted to prevent, delay or reverse the agingof cells, tissues, organs and bodies and prolong the telomere length of the cells, and has the advantages of clear action target, clear, mature and stable composition, and accuracy in regulation of the anti-aging process; and the quality control and the large-scale production of the small-molecular compound are easy. The bodies or cells do not express dry genes in the action process, so the safetyis high; and the small-molecular compound and the combination thereof can be easily absorbed by the bodies, can prevent, delay and reverse the aging at the overall level of cells, tissues, organs andbodies, and is of great significance to delaying the process of human aging and improving the health state and the life quality.

Description

technical field [0001] The present invention relates to cell biology, tissue engineering and medicine, in particular to a small molecule compound / combination for preventing, delaying or reversing aging of cells, tissues, organs and organisms, products and uses thereof. Background technique [0002] Aging of the human body is a major health problem in modern society, and it is also the main cause of most senile diseases. The aging process is accompanied by the reduction of body functions, the weakening of the ability to adapt to external changes, the decline of compensatory functions, and finally induces a variety of diseases. Such as cardiovascular diseases, malignant tumors, degenerative diseases of nerves, internal organs, bones and muscles, diabetes, etc. are closely related to aging. Every time the age increases by 10 years, the mortality rate due to cardiovascular diseases will increase by 2 to 3 times. It can be seen that aging has a great impact on the quality of lif...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61K45/00A61K8/96A61Q19/08A61P17/18A23L33/00
CPCA61K45/00A61Q19/08A61K8/96C12N5/0602A23V2002/00C12N2501/135C12N2501/01C12N2500/38C12N2501/385C12N2501/15C12N2501/415C12N2501/72C12N2501/73C12N2501/727C12N2501/71A23V2200/302A23L33/00A61P17/18
Inventor 胡敏李燕皎
Owner 云南美颜美龄生物科技有限公司
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