Construction of silver nano-cluster-based logic gate and application thereof in intelligent detection

A technology of silver nanoclusters and logic detection, applied in the field of molecular logic gates in analytical chemistry, which can solve problems such as complex construction processes

Active Publication Date: 2018-07-20
TAIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few label-free logic sensors based on the molecular level, which is due...

Method used

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  • Construction of silver nano-cluster-based logic gate and application thereof in intelligent detection
  • Construction of silver nano-cluster-based logic gate and application thereof in intelligent detection
  • Construction of silver nano-cluster-based logic gate and application thereof in intelligent detection

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Experimental program
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Effect test

Embodiment 1

[0018] Preparation of silver nanoclusters: Dissolve 5 μM Ag-DNA1 or Ag-DNA2 in sodium phosphate buffer solution (10 mM Na 2 HPO 4 / NaH 2 PO 4 ,100mM CH 3 COONa, 5mM Mg(CH 3 COO) 2 , pH 7.5), then heated at 90 °C for 10 min, and then cooled slowly to room temperature. Then 30 μM AgNO 3 Add to 5μM Ag-DNA1 or Ag-DNA2 solution, after mixing, place the mixed solution in the dark for 30 minutes to make Ag + The ion fully interacts with the C base. Finally, add 30 μM NaBH to the buffer system again 4 solution, shaken vigorously for 1 min. The fluorescent silver nanoclusters were prepared by reacting overnight at 4°C in the dark.

Embodiment 2

[0020] Construction of OR logic gates based on silver nanoclusters and graphene oxide: Based on the molecular logic platform, different combinations of miR-21 and miR-141 were added to construct OR logic gates. When nucleic acid sequences (miR-21 and miR-141) were added, the input was recorded as "1", otherwise "0". Based on whether the relative fluorescence intensity value is greater than 0.5 or not, when the relative fluorescence intensity is greater than 0.5, the output is "1", and when the relative fluorescence intensity is less than 0.5, the output is "0". When there is no input, the fluorescence of silver nanoclusters is quenched by graphene oxide, and the relative fluorescence intensity is less than 0.5, which is recorded as "0" as the output. Since the sequences of miR-21 and Ag-DNA1 are partially complementary, when miR-21 is added, Ag-DNA1 will hybridize with miR-21 to form a DNA / RNA double-stranded structure, which will desorb from the surface of graphene oxide . ...

Embodiment 3

[0022]Construction of INHIBIT logic gates based on miR-21 and P1: Based on the molecular logic platform, different combinations of miR-21 and P1 were added to construct INHIBIT logic gates. When nucleic acid sequences (miR-21 and P1) were added, the input was recorded as "1", otherwise "0". Based on whether the relative fluorescence intensity value is greater than 0.5 or not, when the relative fluorescence intensity is greater than 0.5, the output is "1", and when the relative fluorescence intensity is less than 0.5, the output is "0". When there is no input, the fluorescence of silver nanoclusters is quenched by graphene oxide, and the relative fluorescence intensity is less than 0.5, which is recorded as "0" as the output. Since the sequences of miR-21 and Ag-DNA1 are partially complementary, when miR-21 is added, Ag-DNA1 will hybridize with miR-21 to form a DNA / RNA double-stranded structure, which will desorb from the surface of graphene oxide . Therefore, the silver nano...

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Abstract

The invention belongs to the technical field of molecular logic gates in the industry of analytical chemistry, and relates to the construction of a label-free, high-sensitivity and high-selectivity miRNA intelligent detection method. A molecular logic gate is constructed based on silver nano-clusters and graphene oxide. According to the invention, the relative fluorescence intensity of silver nano-clusters is used as a judgment basis, when the relative fluorescence intensity is larger than 0.5, 1 is outputted. When the relative fluorescence intensity is less than 0, 0 is outputted. The molecular logic gate comprises an OR logic gate and an INHIBIT logic gate, and can be used for detecting the presence of two different miRNAs (miR-21 and miR-141) in an actual sample respectively. By adopting the logic detection method, the contents of a plurality of target analytes in a complex biological sample can be identified. Therefore, the invention has a potential application value in the aspectsof early diagnosis, multi-element detection, clinical treatment and the like for major diseases.

Description

technical field [0001] The invention belongs to the technical field of molecular logic gates of analytical chemistry, and relates to the establishment of a label-free, highly sensitive and highly selective miRNA intelligent detection method. Background technique [0002] Molecular devices were proposed in the 1980s and mainly involve two fields: devices based on molecular scale and devices based on molecular materials. Among them, molecular-scale devices are currently one of the most active and widely researched hotspots. Molecular devices are crucial to the miniaturization of analytical chemistry instruments. Molecular devices have the characteristics of low cost and rapid response, which is conducive to the development of portable instruments to achieve real-time and online detection. Molecular devices mainly include molecular rectifiers, molecular memories, molecular motors, and molecular logic gates. This patent mainly uses molecular logic gates and biosensors to perfo...

Claims

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Application Information

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IPC IPC(8): G06N3/12G06N99/00
CPCG06N3/123G06N99/007
Inventor 张思琪韩得满
Owner TAIZHOU UNIV
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