The invention belongs to the technical field of molecular logic gates in the industry of analytical chemistry, and relates to the construction of a label-free, high-sensitivity and high-selectivity miRNA intelligent detection method. A molecular logic gate is constructed based on silver nano-clusters and graphene oxide. According to the invention, the relative fluorescence intensity of silver nano-clusters is used as a judgment basis, when the relative fluorescence intensity is larger than 0.5, 1 is outputted. When the relative fluorescence intensity is less than 0, 0 is outputted. The molecular logic gate comprises an OR logic gate and an INHIBIT logic gate, and can be used for detecting the presence of two different miRNAs (miR-21 and miR-141) in an actual sample respectively. By adopting the logic detection method, the contents of a plurality of target analytes in a complex biological sample can be identified. Therefore, the invention has a potential application value in the aspectsof early diagnosis, multi-element detection, clinical treatment and the like for major diseases.