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Fabrication method of a scalable sleeve-type microfluidic chip

A microfluidic chip and sleeve technology, applied in the field of microfluidics, can solve problems such as hindering the promotion of microfluidic chips, and achieve the effects of realizing coaxial 3D printing, simple production, and convenient parameter adjustment.

Active Publication Date: 2019-10-11
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These complex operations are contrary to the simple and convenient advantages of the capillary glass sleeve itself, which hinders the promotion of this type of microfluidic chip.

Method used

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  • Fabrication method of a scalable sleeve-type microfluidic chip

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Experimental program
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Effect test

preparation example Construction

[0025] This specific embodiment discloses a method for preparing an expandable sleeve-type microfluidic chip, such as figure 1 shown, including the following steps:

[0026] S1: Put the prepolymer that matches the size of the channel into the PDMS prepolymer, polymerize the PDMS by heating, and cut it into a PDMS block; the PDMS block is made of polydimethylsiloxane. PDMS is the English abbreviation of polydimethylsiloxane.

[0027] S2: remove the prefabricated material, leaving a pipe groove for placing the channel, and the pipe groove has two nozzles. The material matching the outer dimension of the capillary glass tube is used as the pre-set, and the pre-set is removed by taking out directly or corroding with corrosive liquid.

[0028] S3: Use the chamfered and polished dispensing syringe to open a hole in the direction of the PDMS block perpendicular to the pipe groove. The size of the through hole is larger than the size of the pipe groove.

[0029] S4: Bonding the tw...

Embodiment 1

[0033] Take the microfluidic chip with two-stage casing as an example. A capillary glass tube with an outer diameter of about 0.55 mm and an inner diameter of 0.3 mm is used to make a microfluidic channel, and a steel needle with an outer diameter of about 0.51 mm is used as a preset, and the preset is removed by directly pulling it out. Dow Corning 184 was used as the PDMS prepolymer with a prepolymer ratio of 10:1. Use an alcohol lamp to draw a capillary glass tube, and the outer diameter of the front end of the capillary glass tube is 80um. The microfluidic chip was bonded using an oxygen plasma treatment method. This method is used to fabricate a two-stage casing microfluidic chip.

[0034] S11: After fully stirring the prepolymer in a clean beaker until fine and uniform bubbles appear, place the beaker in a vacuum desiccator to exhaust the air, pour it in the area limited by the aluminum frame on the clean glass, put and fix the steel needle Position in the middle of t...

Embodiment 2

[0041] Take the microfluidic chip with dual-channel parallel single-stage casing as an example. A capillary glass tube with an outer diameter of about 0.55 mm and an inner diameter of 0.3 mm is used to make a microfluidic channel, and a steel needle with an outer diameter of about 0.51 mm is used as a preset, and the preset is removed by directly pulling it out. Dow Corning 184 was used as the PDMS prepolymer with a prepolymer ratio of 10:1. Use an alcohol lamp to draw a capillary, and the diameter of the front end of the capillary is 80um. The microfluidic chip was bonded using an oxygen plasma treatment method. The method is used to fabricate a microfluidic chip with double-channel parallel single-stage sleeves.

[0042] S21: After fully stirring the prepolymer in a clean beaker until fine and uniform air bubbles appear, place the beaker in a vacuum desiccator to exhaust the air, pour it into the area limited by the aluminum frame on the clean glass, place and fix two The...

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Abstract

The invention discloses a preparation method of an expandable sleeve-type micro-fluidic chip, which comprises the following steps of: S1: placing a preset object matched with a passage size into PDMSprepolymer, carrying out heating and polymerization on the PDMS, and cutting the PDMS into PDMS blocks; S2: removing the preset object, and reversing a tube seat for placing a passage, wherein the tube seat is provided with two tube orifices; S3: using an dispensing syringe subjected to chamfering polishing, and opening through holes on the PDMS blocks in a direction perpendicular to the tube seat; S4: respectively bonding both surfaces of the PDMS blocks, which are provided with the through holes, with a substrate and a top layer, wherein a sampling hole is preset on the top layer; S5: inserting a capillary glass tube with a uniform inner diameter from one tube orifice of the tube seat, inserting a capillary glass tube subjected to projection in advance from the other tube orifice of thetube seat, and completing manufacturing of a single-stage sleeve-type micro-fluidic chip; and S6: reusing the capillary glass tubes and repeating the step S5 to form a multi-stage sleeve structure. According to the invention, the operation difficulty and economic cost of manufacturing of the sleeve-type micro-fluidic chip are effectively reduced.

Description

technical field [0001] The invention relates to the field of microfluidics, in particular to a method for preparing an expandable sleeve-type microfluidic chip. Background technique [0002] In recent years, microfluidic chip technology based on microfabrication technology has become one of the hottest cutting-edge analytical technologies in recent years due to its fast analysis speed, low reagent consumption, easy integration and high-throughput analysis. The selection of drug targets, drug screening, preclinical tests and determination of doses provide a new platform. [0003] Compared with the previous macroscopic liquid manipulation technology, the unique advantage of microfluidic technology is that at the micro scale, the flow characteristics of the liquid change, such as the laminar flow phenomenon with low Reynolds number and the manipulation of liquid droplets. Traditional microfluidic chips generally have a planar structure, that is, the height of each channel is t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B81C3/00
Inventor 王著元杨阔崔一平宗慎飞
Owner SOUTHEAST UNIV