Method for separating high-purity saponin monomers from tea seeds

A tea seed saponin, high-purity technology, applied in steroids, organic chemistry, etc., can solve the problems of low separation efficiency, large blindness, large sample consumption, etc., and achieve a simple separation process, strong pertinence, and repeatability. good effect

Active Publication Date: 2018-07-27
ZHEJIANG UNIV +1
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Problems solved by technology

Its main disadvantages are large sample consumption, cumbersome operation in the separation process, e

Method used

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Embodiment Construction

[0026] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited thereto.

[0027] (1) Pick the tea seeds of the Fuding Dabai variety, remove the shells, and dry them in a dry constant temperature oven at 60°C until they reach a constant weight. The seeds are crushed by a plant grinder, passed through a 20-mesh sieve, and the sun-dried tea seed powder is collected.

[0028] (2) Take 1 kg of tea seed powder, add 70% ethanol at a ratio of 1:15 (w / v), extract under reflux at 70°C for 5 hours, collect the extract by suction filtration while it is hot, and concentrate under vacuum rotation at 45°C to obtain an extract (Yield about 13.25%).

[0029] (3) Dissolve the extract in 1L of water, extract three times with 1:1 (v / v) petroleum ether, and collect the aqueous phase solution; extract three times with 1:1 (v / v) ethyl acetate to collect the aqueous phase solution; 1:1 (v / v) Extract...

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Abstract

The invention specifically relates to a method for separating high-purity saponin monomers from tea seeds, belonging to the field of separation and purification of natural compounds. In particular, the method involves separation and purification of six high-purity pentacyclic triterpene oleanane saponins in tea seeds. Compared with the prior art, the method provided by the invention has the following beneficial effects: (a) the purity of the compounds are high, as high as 91 to 99%; (b) separation process is simple, and six main monomeric saponin substances in tea seeds can be obtained at thesame time; and (c) the method has good repeatability and is well targeted.

Description

technical field [0001] The present invention relates to the field of separation, purification and preparation of natural compounds, in particular to a method for separating high-purity tea seed saponin monomer compounds, and relates to the preparation of six high-purity pentacyclic triterpene oleanane-type saponins in tea seeds Isolation and Purification. Background technique: [0002] Tea (Camellia sinensis (L.) O.Kuntze) seeds are another important tea tree resource besides tea leaves. They are rich in triterpene saponins, which can regulate the stomach, antibacterial and anti-inflammatory, anti-tumor, protect nerves, promote plant absorption, Bioactivity and functions such as foaming and decontamination have great application prospects in the fields of food, medicine, agriculture, and chemical industry. The structure of saponin in tea seeds is similar in polarity, so it is very difficult to separate and prepare pure monomers. The lack of pure monomers seriously hinders ...

Claims

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Application Information

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IPC IPC(8): C07J63/00
CPCC07J63/008
Inventor 李博屠幼英吴学进贾玲燕蔡宗敏徐碧霞
Owner ZHEJIANG UNIV
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