Method for quickly screening and confirming common pesticides and rodenticides in poisoning samples of complex matrixes

A technology for complex matrices and samples, used in measuring devices, instruments, scientific instruments, etc., to shorten detection and processing time, improve detection efficiency, and simplify operation.

Inactive Publication Date: 2018-07-27
济南市疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the inability to quickly and accurately qualitative and quantitative, the current detection methods are not enough to meet the actual needs

Method used

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  • Method for quickly screening and confirming common pesticides and rodenticides in poisoning samples of complex matrixes
  • Method for quickly screening and confirming common pesticides and rodenticides in poisoning samples of complex matrixes
  • Method for quickly screening and confirming common pesticides and rodenticides in poisoning samples of complex matrixes

Examples

Experimental program
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Effect test

Embodiment 1

[0079] Example 1: In a collective food poisoning incident, the patients generally experienced symptoms of dizziness, limb weakness, bleeding gums, and hematuria. The doctor suspected rat poisoning, and collected the patient's blood and urine for screening and testing. The specific steps are:

[0080] (1) Sample extraction: Accurately measure 5mL of blood and urine samples into a 50mL stoppered centrifuge tube, add acetonitrile (containing 1% acetic acid and 10% acetone) for homogeneous extraction for 1min, add 2.0g of anhydrous magnesium sulfate and 1.5g Sodium acetate, shake and centrifuge, take the supernatant to be purified.

[0081] (2) Sample purification

[0082] Take 3mL supernatant and add 100mgPSA and 300mg MgSO 4 After purification, after centrifugation, the supernatant was passed through a 0.22 μm PTFE filter membrane and analyzed on the machine.

[0083] (3) On-machine detection: Screen and detect samples by ultra-high performance liquid chromatography-triple qu...

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PUM

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Abstract

The invention discloses a method for quickly screening and confirming common pesticides and rodenticides in poisoning samples of complex matrixes. The method comprises the steps as follows: 1) a second-level mass spectrum database, including Chinese names, English names, CAS numbers, molecular weights, molecular formulas, retention time, quantitative ion pairs, qualitative ion pairs and collisionvoltage, of common pesticides is constructed by use of gas chromatography triple quadrupole tandem mass spectrometry and ultrahigh performance liquid chromatography triple quadrupole tandem linear iontrap mass spectrometry; a second-level mass spectrum database of common rodenticides is constructed by use of ultrahigh performance liquid chromatography triple quadrupole tandem linear ion trap massspectrometry; 2) samples causing food poisoning are extracted and purified, detection is performed through gas chromatography triple quadrupole tandem mass spectrometry and ultrahigh performance liquid chromatography triple quadrupole tandem linear ion trap mass spectrometry respectively, and detection conditions are identical with those of a method adopted during construction of the second-levelmass spectrum database.

Description

technical field [0001] The invention relates to a method for rapid screening and confirmation of common pesticides and rodenticides, which is especially suitable for the detection of food poisoning samples of complex matrices. Background technique [0002] Food poisoning refers to non-infectious acute and subacute diseases that occur after ingesting food containing biological and chemical toxic substances or taking toxic and harmful food as food. Food poisoning caused by ingesting chemically poisonous food is chemical food poisoning, among which rat poison and pesticide poisoning are the most common. Food poisoning is one of the main problems in the field of public health and food safety in my country at this stage. Chemical food poisoning caused by toxic and harmful substances not only seriously endangers people's health, but also causes adverse social impacts, affecting a wide area and involving many people. Therefore, determining the cause of poisoning in the first time...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 曹小丽刘素华杨晓倩刘仲焦海涛张鑫
Owner 济南市疾病预防控制中心
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