Biological organic slow-release fertilizer, preparation method and application thereof in soil remediation
A slow-release fertilizer, organic technology, applied in the field of bio-organic fertilizer, can solve problems such as damage to the liver, fishy smell, danger, etc., and achieve the effect of improving fertility
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Embodiment 1
[0043] Embodiment 1 The preparation of bio-organic slow-release fertilizer
[0044] In the first step, the cyanobacteria and water are mixed according to the weight ratio of 1:5, and the cyanobacteria are crushed under the action of mechanical crushing and external ultrasonic waves. The power of the external ultrasonic waves is 2500W and the frequency is 10kHz; the obtained suspension Dilute 2 times, add HCl to the diluent to adjust the pH to 3, then add 0.05wt% enzyme of the diluent weight to carry out enzymolysis, the enzymolysis reaction time is 3h, the reaction temperature is 35°C, after the enzymolysis reaction, after 95 °C inactivating enzyme treatment, the enzyme is a mixture of alkaline cellulase, alkaline xylanase and laccase in a weight ratio of 3:1:0.5;
[0045] In the second step, add strains to the enzymolysis liquid obtained in the first step for fermentation to obtain a cyanobacteria fermentation liquid, the strains are a mixture of Saccharomyces cerevisiae and ...
Embodiment 2
[0050] Embodiment 2 Preparation of bio-organic slow-release fertilizer
[0051] In the first step, the cyanobacteria and water are mixed according to the weight ratio of 1: 10, and the cyanobacteria are crushed under the action of mechanical crushing and external ultrasonic waves. The power of the external ultrasonic waves is 4000W and the frequency is 30kHz; the obtained suspension Dilute 4 times, add HCl to the diluent to adjust the pH to 5, and then add 0.1wt% enzyme of the diluent weight for enzymolysis. ℃ inactivating enzyme treatment, the enzyme is a mixture of alkaline cellulase, alkaline xylanase and laccase in a weight ratio of 4: 1.2: 0.8;
[0052] In the second step, add strains to the enzymolysis liquid obtained in the first step for fermentation to obtain a cyanobacteria fermentation liquid, the strains are a mixture of Saccharomyces cerevisiae and Lactobacillus acidophilus, the fermentation temperature is 35°C, and the fermentation time is 100h;
[0053] The 3rd...
Embodiment 3
[0057] Embodiment 3 Preparation of bio-organic slow-release fertilizer
[0058] In the first step, the cyanobacteria and water are mixed according to the weight ratio of 1:7, and the cyanobacteria are crushed under the action of mechanical crushing and external ultrasonic waves. The power of the external ultrasonic waves is 3000W and the frequency is 20kHz; the obtained suspension Dilute 3 times, add HCl to the diluent to adjust the pH to 4, then add 0.08wt% enzyme in the diluent to carry out enzymolysis, the enzymolysis reaction time is 3~5h, the reaction temperature is 35~38℃, the enzymolysis reaction is completed Afterwards, the enzyme is treated at 95°C, and the enzyme is a mixture of alkaline cellulase, alkaline xylanase and laccase in a weight ratio of 4:1:0.6;
[0059] In the second step, add strains to the enzymolysis liquid obtained in the first step for fermentation to obtain a cyanobacteria fermentation liquid, the strains are a mixture of Saccharomyces cerevisiae a...
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