Application of epimedium extract in preparation of medicine for treating brain nerve injury and cognitive impairment after radiation
A technology of epimedium extract and nerve damage, which is applied in the field of medicine, can solve problems such as the protective effect of epimedium on the brain nerves that have not been mentioned, and achieve the effect of great promotion value
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Embodiment 1
[0025] The preparation of embodiment 1 epimedium extract
[0026] Take the dried whole herb of Epimedium herb, crush it, add 70% ethanol according to the ratio of 1:20, soak it for 0.5h before decocting, decoct twice, filter, combine the filtrate obtained by decocting three times, and concentrate to a thick paste , dried, and stored at 4°C for later use. Epimedium of the present invention specifically refers to the Berberidaceae plant Epimedium.
Embodiment 2
[0027] Example 2 Epimedium extracts treat cognitive impairments such as memory loss and learning ability reduction caused by radiation
[0028] 1. Prepare experimental animals
[0029] 8-week-old SPF-grade male Balb / c mice, body weight (24±2) g, were purchased from Hubei Experimental Animal Research Center, production license number: SCXK (E) 2015-0018. Animals were housed in separate cages at the Experimental Animal Center of Changjiang University School of Medicine. Adaptive feeding for a week, 10 groups per cage.
[0030] The animals were divided into two groups: normal group with 10 animals and radiation model group with 30 animals. The radiation model group was given X-ray one-time whole-body irradiation, which was irradiated by X-Rays, a Swedish Elekta precise medical electron linear accelerator, with a dose of 5.5Gy, an absorbed dose of 550mu / min, a field of view of 15cm×15cm, The distance is 100cm. After the model was successful, they were randomly divided into 3 g...
Embodiment 3
[0048] Example 3 Immunohistochemical experiment - verifying that Epimedium extract treats cranial nerve injury
[0049] Radiation can have serious effects on brain development, such as behavioral changes and cognitive impairments such as memory loss and reduced ability to learn. The proliferation, migration, differentiation, and death of neurons mainly occur in the subventricular zone (SVZ) of the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus. Hippocampal neurons are the basis of spatial memory, and spatial information in the hippocampus Processing is the key, and these are very important for the storage and organization of memories. Proliferating hippocampal neurons are very sensitive to radiation, so we chose four markers on hippocampal neurons, PV, Ki67, DCX and CB.
[0050] Six weeks after irradiation, the mice were anesthetized with 1% pentobarbital, perfused from the mouse heart, fixed with 4% neutral formaldehyde, and the cerebellum was excised ...
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