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Applications of stem cells in heart transplantation model

A heart transplantation and stem cell technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve the problem of impaired global cardiac function, malignant arrhythmia, and cannot reduce the mortality and morbidity of myocardial infarction and heart failure. and other problems, to achieve the effect of maintaining activity, promoting activity, and promoting stem cell proliferation

Inactive Publication Date: 2018-08-10
广州大图技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although drug development and interventional therapy have made great progress, it is still unable to reduce the total mortality and morbidity of heart failure caused by myocardial infarction. Severe failure or malignant arrhythmia will eventually lead to the death of the patient; on the other hand, the intervention of drugs and interventional therapy makes a considerable number of patients with acute myocardial infarction survive, but the dead cells do not have new cells to replace their functions. During compensation, the function of the left ventricle and even the whole heart is severely damaged

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] S2. Preparation of platelet fibrin scaffold;

[0041] S3. Observation of platelet fibrin scaffold;

[0042] S4. Cardiac myosphere-derived cells-platelet fibrin scaffold combined transplantation.

[0043] Preferably, the culturing of cardiac myosphere-derived cells (CDCs) in the step S1 includes the following steps: taking healthy clean-grade female 8-week-old WKY rat myocardial tissue, harvesting the cells by tissue block culture, and then culturing the cells by suspension culture and / or adherent culture to obtain rat cardiac myosphere-derived cells, observe cell morphology in vitro, observe cell viability by continuous passage, and draw cell growth curves; use flow cytometry to detect surface markers of different sources and culture passages VEGF, IGF-1, SDF-1 and cell expression.

[0044] Preferably, the preparation of the platelet fibrin scaffold in step S2 further includes the following steps:

[0045] S1. Use isoflurane to deeply anesthetize the rat;

[0046] S...

experiment example 1

[0062] Experimental Example 1 In vivo experiment

[0063] (1) Experimental grouping and sampling time design

[0064] The first group (DMEM, the conventional medium group) was injected with 100 ul DMEM at the 3 o'clock, 6 o'clock, 9 o'clock and 12 o'clock positions around the infarcted myocardium of rats with acute myocardial infarction, a total of 15 rats;

[0065] The second group (platelet fibrin scaffold group) was injected with 100ul dyed platelet fibrin scaffolds at the 3 o'clock, 6 o'clock, 9 o'clock and 12 o'clock positions around the infarcted myocardium of acute myocardial infarction rats, totaling 15 rats;

[0066] The third group (platelet fibrin scaffold + CDCs group) was injected with 100 ul of unstained platelet fibrin scaffold containing CDCs at the 3 o'clock, 6 o'clock, 9 o'clock and 12 o'clock positions around the infarcted myocardium of rats with acute myocardial infarction.

[0067] The experimental sampling time points were the 12th hour, the 3rd day, the...

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PUM

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Abstract

The invention specifically discloses applications of stem cells in a heart transplantation model. The process comprises the following steps: S1, culturing cardiosphere-derived cells (CDCs); S2, preparing a platelet-rich fibrin scaffold; S3, observing the platelet-rich fibrin scaffold; and S4, carrying out combined transplantation of the cardiosphere-derived cells and the platelet-rich fibrin scaffold. The result shows that the cardiac function after myocardial infarction can be well corrected after the CDCs are combined in the platelet-rich fibrin scaffold, meanwhile, the platelet-rich fibrinscaffold and the CDCs can realize the mutual promotion effect, after combined transplantation, more vascular endothelial cells and myocardial cells can be regenerated, the blood vessel density at theinfarction part can be increased, more stem cells are gathered to enter the infarction area for achieving the regeneration function, thus the negative cardiac remodeling is reversed, and the cardiac function is improved.

Description

technical field [0001] The invention relates to the technical field of heart transplantation models, in particular, to the application of stem cells in a heart transplantation model. Background technique [0002] Myocardial infarction and heart failure are one of the main causes of human death in both Chinese and Western countries, and they seriously increase the socioeconomic burden. Despite the great progress in drug development and interventional therapy, the overall mortality and morbidity of heart failure caused by myocardial infarction still cannot be reduced. Severe failure or malignant arrhythmia eventually leads to death of patients; on the other hand, the intervention of drugs and interventional therapy allows a considerable number of patients with acute myocardial infarction to survive, but the dead cells do not have new cells to replace their functions. During compensation, left ventricular and even whole heart function is severely impaired. Therefore, finding ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/22A61L27/50C12N5/077
Inventor 邱林新
Owner 广州大图技术开发有限公司
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