Application of exosome secreted by human umbilical cord mesenchymal stem cell in preparing medicine for repairing skin ultraviolet injury

A technology of mesenchymal stem cells and skin repair, applied in the field of medicinal effect of vesicle-structured exosomes in promoting the repair of skin photodamage, can solve the problems of multiple allergic reactions and limited repair effects, and achieve the goal of promoting proliferation and inhibiting cell apoptosis Death, the effect of promoting collagen synthesis

Inactive Publication Date: 2018-08-14
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the sunscreens currently on the market for UV prevention and treatment have added antioxidant and mo

Method used

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  • Application of exosome secreted by human umbilical cord mesenchymal stem cell in preparing medicine for repairing skin ultraviolet injury
  • Application of exosome secreted by human umbilical cord mesenchymal stem cell in preparing medicine for repairing skin ultraviolet injury
  • Application of exosome secreted by human umbilical cord mesenchymal stem cell in preparing medicine for repairing skin ultraviolet injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1, Separation and purification of exosomes secreted by umbilical cord mesenchymal stem cells;

example 1

[0027] The main materials and sources used in Example 1 are as follows:

[0028] MSC culture reagents: low-sugar DMEM, fetal bovine serum (product of Gibco company), trypsin (product of Sigma company), carbon dioxide incubator (forma company), serum-free medium (Shanghai Yikesai company)

[0029] Inverted microscope, biological microscope, ultra-clean workbench, desktop centrifuge;

[0030] exosome extraction reagent EXOTC10A-1 (SBI company), rabbit anti-human CD9 antibody (American BioworldTechnology company), rabbit anti-human CD63 antibody (American Abcam company), BCA protein quantitative kit, horseradish peroxidase (HRP) labeled Goat anti-rabbit IgG secondary antibody (Beijing Kangwei Century Company), premixed HRP chemiluminescence substrate, 100-kDaMWCO ultrafiltration centrifuge tube, 0.22μm sterile filter membrane (Millipore Company, USA);

[0031] Anti-human CD9 antibody and rabbit anti-human CD63 antibody (Abcam);

[0032] As attached figure 1 , The specific implementation s...

Embodiment 2

[0038] Example 2. The effect of exosome secreted by umbilical cord mesenchymal stem cells on the repair of SD rat skin UV damage;

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Abstract

The invention belongs to the technical field of skin light damage repair, and discloses application of exosome secreted by a human umbilical cord mesenchymal stem cell to preparation of a medicine forrepairing skin ultraviolet injury. The umbilical cord mesenchymal stem cell exosome is a natural vesica, so that the immunogenicity is lower. Meanwhile, the exosome has functions of oxidation resistance, antiinflammatory effect, proliferation, apoptosis inhibition, collagen synthesis and the like similar to the functions of a parent cell in tissue regeneration repair, and can play a role in skinprotection in multiple ways. Therefore, the exosome hopefully become an alternative strategy for decellularization treatment, and has important clinic application prospect and market transformation value in multiple skin regeneration related diseases.

Description

Technical field [0001] The present invention belongs to the technical field of skin photodamage repair. More specifically, the present invention relates to a vesicle structure exosome with a diameter of about 100nm secreted by umbilical cord mesenchymal stem cells for medicinal use in promoting skin photodamage repair effect. Background technique [0002] With the destruction of the ozone layer, its ability to absorb ultraviolet rays decreases, and people's chances of receiving excessive ultraviolet radiation are greatly increased. Excessive ultraviolet radiation can lead to light sunburn and photoaging of the skin, and it also greatly increases the incidence of skin cancer. Not only affects the appearance, but also endangers the patient's life. According to incomplete statistics, about 100,000 people die from skin cancer every year in major areas of the world. Most of the cases are related to excessive exposure to ultraviolet radiation. Because every 1% of ozone in the ozone ...

Claims

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Application Information

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IPC IPC(8): A61K35/51A61P17/16
CPCA61K35/51A61P17/16
Inventor 吴佩佩
Owner JIANGSU UNIV
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