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Sowing method applicable to small-diameter seed indoor germination test and application of sowing method

A seed chamber and trail technology, applied in application, seed and rhizome treatment, agriculture, etc., can solve problems such as blocked absorption, easy aggregation, and reduced germination rate, so as to provide energy and nutritional support, improve material metabolism rate, The effect of improving the germination rate of seeds

Inactive Publication Date: 2018-08-17
HENAN UNIV OF SCI & TECH
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  • Claims
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Problems solved by technology

However, for seeds with a diameter of millimeters, such as Henan Tuchuanghua seeds, when carrying out the germination test in the above-mentioned traditional mode, the seeds are too small, and after adding the treatment solution (or distilled water), the seeds are easy to gather together, and It will inevitably "flow" around with the treatment liquid on the filter paper, the absorption of water, light, air and even nutrients will be blocked, and the mutual influence between seeds will have a negative effect on seed germination, which is not conducive to seed germination, thereby reducing the germination rate
This method is not conducive to the further progress of seed germination experiments.
[0004] Secondly, the above-mentioned "petri dish + filter paper" sowing method, the seeds gather, it is not convenient to count the germination rate, especially the germination potential
In addition, due to the small size of the seeds, each time the treatment solution (or distilled water) is added, the seeds "flow" around, which will further increase the statistical difficulty of germinated seeds
In addition, the commonly used sand culture method for seed germination has the problem that the color of the seeds is close to that of sand, which is not convenient for later observation of seeds.
In addition, another important problem is that the germination test of some plant seeds is often 7 days or longer, and the loss of water volatilization is inevitable, and the existing method is only a simple cover treatment of the petri dish, which cannot maintain the germination well. moisture

Method used

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  • Sowing method applicable to small-diameter seed indoor germination test and application of sowing method
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  • Sowing method applicable to small-diameter seed indoor germination test and application of sowing method

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Embodiment 1

[0027] 1. Seed treatment

[0028] Seeds were collected in mid-May, and then placed in a 4°C refrigerator for low-temperature storage. Seed germination trials began in September. Take a certain amount of seeds and put them into a 500mL beaker with distilled water at 50°C. After soaking for 24 hours, remove the floating seeds and take the sinking seeds as the test object. The seed germination test without any treatment was used as the control (CK), and the rest were treated as follows: ① HCl treatment: three concentration gradients of 5%, 10%, and 20%, respectively, for 0.5 h, 1 h, and 2 h, and then 1 h % sodium hypochlorite solution soaked for 10 minutes for disinfection, after repeated rinsing with distilled water, the room was naturally dried and seeded. According to the germination rate of the seeds, the optimal HCl treatment concentration and time are selected, and the HCl of this concentration is used to treat the bald acne seeds, and then the gibberellin treatment is ca...

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Abstract

The invention relates to a sowing method applicable to a small-diameter seed indoor germination test and an application of the sowing method, and belongs to the technical field of plant sowing. The sowing method includes the steps: vertically placing a filter paper roll in a disinfected culture dish close to the edge of the disinfected culture dish; laying fine sand in the culture dish, and stacking filter paper above the fine sand to form a filter paper layer; forming needle holes penetrating the whole filter paper layer in the uppermost filter paper, and sowing seeds in the needle holes; wrapping the culture dish by the aid of a preservative film, enabling the filter paper roll to be exposed, adding water or treatment liquid into the filter paper roll until the filter paper roll is completely soaked, covering a culture dish cover, and placing the culture dish into a culture box. The sowing method is applied to an indoor germination test of dicranostigma henanensis S.Y.Wang et L. H.Wu, the sowing method is combined with a treatment method of dicranostigma henanensis S.Y.Wang et L. H.Wu seeds, and the germination rate of the dicranostigma henanensis S.Y.Wang et L. H.Wu seeds can beremarkably increased, so that dicranostigma henanensis S.Y.Wang et L. H.Wu is further tested and researched.

Description

technical field [0001] The invention belongs to the technical field of plant sowing, and in particular relates to a sowing method suitable for indoor germination tests of small-diameter seeds and an application thereof. Background technique [0002] Henan bald sore flower ( Dicranostigma henanensis S.Y.Wang et L. H.Wu.), was discovered by Wu Lihong et al. in Nanshan, Luoyang City, Henan Province in 1997, and identified as a new species of the genus Papaveria genus, published in academic journals such as "Botanical Research", and currently the The plant is only found in the Henan region. This species is a perennial herb that performs well in arid areas, with a flowering period of March-May and a fruiting period of May-July. The plant is tall and has many branches. During the flowering season, there are many flowers, and the calyx is green and yellow. It has high ornamental value and is expected to be domesticated and used as a flower border grass. The capsule of Henan Tuc...

Claims

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Application Information

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IPC IPC(8): A01C1/00
CPCA01C1/00
Inventor 王宁袁美丽陈浩董莹莹靳利红孙仁昌赵晓红丁博张林姚孝其李真真熊晓燕高毅
Owner HENAN UNIV OF SCI & TECH
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