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Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils

A technology of rhodococcus and bacteria agent, which is applied in the application field of rhodococcus YC915 and its adsorbed bacteria agent in degrading soil phthalate, which can solve the problems of phthalate pollution and achieve high degradation efficiency , simple operation, high-efficiency degradation effect

Inactive Publication Date: 2018-08-17
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problems referred to above, the object of the present invention is to provide a strain for phthalates (especially DBP and DEHP) has the bacterial strain of efficient degradability, and then provide a kind of adsorption bacterial agent preparation method of DBP, DEHP in degrading soil, Moreover, the preparation method of the adsorbing bacteria agent is simple to operate, is suitable for wide application, and can solve the problem of phthalate pollution in farmland soil

Method used

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  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils
  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils
  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1 Screening and Identification of Phthalate Degrading Bacteria

[0035] (1) Screening of phthalate-degrading bacteria

[0036] A certain amount of soil samples were collected in vegetable greenhouses in the suburbs of Yinchuan City, and 10g of fresh soil samples were accurately weighed, added to a conical flask filled with 90mL of distilled water and 10mL of glass beads, and placed in a shaker at 30°C and 175rpm to vibrate and mix well. Set aside.

[0037] Take 1mL from the above mixed solution according to the inoculum size of 1%, add it to 100mL inorganic salt culture solution with an initial concentration of 100mg / L phthalate (both DBP and DEHP are 50mg / L), and place it on a shaker Shaking culture was carried out at 30°C and 175rpm in the dark for 7 days. Gradually increase the concentration of phthalates (including 200mg / L, 500 mg / L, 800mg / L, 1000mg / L, 1500mg / L and 2000mg / L) for each transfer, transfer once every seven days as an acclimatization cycle , c...

Embodiment 2

[0042] Example 2 Detection of Rhodococcus YC915's ability to degrade phthalates

[0043] Preparation of bacterial suspension: Pick smooth and complete colonies of Rhodococcus YC915, inoculate them into beef extract peptone liquid medium, and cultivate them in a shaking table at 28°C and 170r / min for 24 hours. After taking out the beef extract peptone liquid medium, put the culture medium in a sterilized centrifuge tube, and centrifuge at 4000r / min for 10min at room temperature to collect wet bacteria. Then wash three times with sterile physiological saline (0.9% NaCl solution). Finally, the bacterium was formulated into a bacterium suspension with physiological saline, so that the absorbance value OD=1 under the condition of wavelength λ=600nm.

[0044] Determination of residual amount of DBP / DEHP in DBP / DEHP inorganic salt medium: Add 30ml of ethyl acetate to 100ml of DBP / DEHP inorganic salt medium, shake for 30min, then transfer to a separatory funnel and shake for 5min to ...

Embodiment 3

[0061] The optimization of embodiment 3 adsorption bacteria preparation conditions

[0062] (1) Preparation of bacterial suspension: Inoculate strain YC915 into beef extract-peptone liquid medium, and culture at 28° C., 170 r / min for 24 hours. After taking out the liquid medium, divide the liquid into 50 mL sterile centrifuge tubes, and centrifuge at 4000 rpm for 10 min. After discarding the supernatant, resuspend with sterilized normal saline and centrifuge again. After washing twice, prepare the bacterial suspension with normal saline to make the OD 600 = 1.0.

[0063] (2) The preparation method of the adsorption bacteria agent: accurately weigh a certain amount of adsorption material biochar and pour it into a conical flask, add a certain volume of bacterial suspension with an OD600=1.0, shake it up and place it at different temperatures at a speed of 175r / h Min shaker shaking culture for a certain period of time, filtered and washed with sterile water until neutral, that...

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Abstract

The invention relates to rhodococcus YC915 and application of an adsorption bacterial agent of the rhodococcus YC915 for degrading phthalate ester in soils. A category name of the rhodococcus YC915 isRhodococcus sp., and the rhodococcus YC915 is preserved in China Center for Type Culture Collection (CCTCC) with address being Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province, a preservation date being February 22, 2018 and a preservation number being CCTCC M 2018094. The rhodococcus YC915 provided by the invention has an efficient degradation effect on DBP and DEHP composite pollution of the soils and has a wide application prospect in the field of phthalate ester polluted soil repair.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of polluted soil, and particularly relates to the application of a strain of rhodococcus YC915 and its adsorbing bacteria agent in degrading soil phthalates. Background technique [0002] Phthalic acid esters (PAEs), also known as phthalic acid esters, are an important class of synthetic organic compounds, mostly colorless and transparent oily liquids, hardly soluble in water, easily soluble in organic solvents, and difficult to volatilize And with high fat solubility. It is widely used in industries such as plastics, chemical fertilizers, pesticides, toys, cosmetics, detergents and paints. About 85% of PAEs are used in the processing and manufacturing of polyvinyl chloride (PVC) as a plasticizer to improve the extension of plastic products. and softness. Due to the widespread use of phthalate-containing products, PAEs residues have been detected in various matrices such as soil, w...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/01
CPCB09C1/10B09C2101/00C12N1/20C12N11/14C12N1/205C12R2001/01
Inventor 王亚娟
Owner NINGXIA UNIVERSITY
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