Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils

A technology of rhodococcus and bacteria agent, which is applied in the application field of rhodococcus YC915 and its adsorbed bacteria agent in degrading soil phthalate, which can solve the problems of phthalate pollution and achieve high degradation efficiency , simple operation, high-efficiency degradation effect

Inactive Publication Date: 2018-08-17
NINGXIA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the problems referred to above, the object of the present invention is to provide a strain for phthalates (especially DBP and DEHP) has the bacterial strain of efficient degradability, and then provide a kind of adsorption bacter

Method used

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  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils
  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils
  • Rhodococcus YC915 and application of adsorption bacterial agent of rhodococcus YC915 for degrading phthalate ester in soils

Examples

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Example Embodiment

[0034] Example 1 Screening and identification of phthalate ester degrading bacteria

[0035] (1) Screening of phthalate ester degrading bacteria

[0036] Collect a certain amount of soil samples in a vegetable greenhouse in the suburbs of Yinchuan City, accurately weigh 10g of fresh soil samples, add them to a triangular flask containing 90mL of distilled water and 10mL of glass beads, and place them in a shaker at 30°C and 175rpm to shake and mix. Set aside for use.

[0037] Take 1 mL from the above mixed solution at 1% of the inoculum amount, add it to 100 mL of inorganic salt culture solution with an initial concentration of 100 mg / L phthalate (both DBP and DEHP are 50 mg / L), and place on a shaker Shake culture for 7 days at 30°C and 175 rpm in the dark. Each transfer gradually increases the phthalate concentration (including 200mg / L, 500 mg / L, 800mg / L, 1000mg / L, 1500mg / L and 2000mg / L), and transfers once every seven days as a domestication cycle , After a total of 6 acclimatiz...

Example Embodiment

[0042] Example 2 Detection of phthalate degradation ability of Rhodococcus YC915

[0043] Preparation of bacterial suspension: pick the smooth and intact colony of Rhodococcus YC915, inoculate it into the beef extract peptone liquid medium, and culture it in a shaker at 28°C and 170r / min constant temperature shaking for 24h. After the beef extract peptone liquid medium is taken out, the culture solution is placed in a sterilized centrifuge tube, and centrifuged at 4000 r / min for 10 minutes at room temperature to collect the wet bacteria. Then it was washed three times with sterilized physiological saline (0.9% NaCl solution). Finally, the bacterial cells are formulated into a bacterial suspension with physiological saline to make the absorbance value OD=1 under the condition of wavelength λ=600nm.

[0044] Determination of the residual amount of DBP / DEHP in the DBP / DEHP inorganic salt medium: Add 30ml of ethyl acetate to 100ml of the DBP / DEHP inorganic salt medium, shake for 30 mi...

Example Embodiment

[0061] Example 3 Optimization of preparation conditions of adsorbent bacteria

[0062] (1) Preparation of bacterial suspension: inoculate the strain YC915 into beef extract peptone liquid medium, and culture it at 28°C and 170r / min for 24h. After removing the liquid culture medium, the liquid was divided into 50 mL sterile centrifuge tubes and centrifuged at 4000 rpm for 10 min. After discarding the supernatant, resuspend it in sterilized saline and centrifuge again. After washing twice, prepare the bacterial suspension with saline to make the OD 600 = 1.0.

[0063] (2) Preparation method of adsorbent bacteria: accurately weigh a certain amount of adsorbent biochar and pour it into an Erlenmeyer flask, add a certain volume of bacterial suspension with OD600 = 1.0, shake it well, and place it at different temperatures and speeds of 175r / Incubate for a certain period of time with shaking in a shaker for min, filter and wash with sterile water to neutrality to obtain the adsorbent b...

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Abstract

The invention relates to rhodococcus YC915 and application of an adsorption bacterial agent of the rhodococcus YC915 for degrading phthalate ester in soils. A category name of the rhodococcus YC915 isRhodococcus sp., and the rhodococcus YC915 is preserved in China Center for Type Culture Collection (CCTCC) with address being Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province, a preservation date being February 22, 2018 and a preservation number being CCTCC M 2018094. The rhodococcus YC915 provided by the invention has an efficient degradation effect on DBP and DEHP composite pollution of the soils and has a wide application prospect in the field of phthalate ester polluted soil repair.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of polluted soil, and particularly relates to the application of a strain of rhodococcus YC915 and its adsorbing bacteria agent in degrading soil phthalates. Background technique [0002] Phthalic acid esters (PAEs), also known as phthalic acid esters, are an important class of synthetic organic compounds, mostly colorless and transparent oily liquids, hardly soluble in water, easily soluble in organic solvents, and difficult to volatilize And with high fat solubility. It is widely used in industries such as plastics, chemical fertilizers, pesticides, toys, cosmetics, detergents and paints. About 85% of PAEs are used in the processing and manufacturing of polyvinyl chloride (PVC) as a plasticizer to improve the extension of plastic products. and softness. Due to the widespread use of phthalate-containing products, PAEs residues have been detected in various matrices such as soil, w...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/01
CPCB09C1/10B09C2101/00C12N1/20C12N11/14C12N1/205C12R2001/01
Inventor 王亚娟
Owner NINGXIA UNIVERSITY
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