Methods for preparing CuO/rGO@PNIPAm (poly(N-isopropylacrylamide)) electrode and structuring non-enzyme glucose sensor

The invention relates to methods for preparing a CuO/rGO@PNIPAm compound self-support electrode and structuring a thermosensitive non-enzyme glucose sensor and an application method of the thermosensitive non-enzyme glucose sensor. The methods for preparing the CuO/rGO@PNIPAm compound self-support electrode and structuring the thermosensitive non-enzyme glucose sensor comprise preparing a CuO/GO compound membrane from porous CuO and graphene; placing the CuO/GO compound membrane into NIPAM monomer aqueous solution, performing polymerization reaction in an ultrasonic-intermittent/N2 inletting circulating manner to prepare a CuO/GO@PNIPAm compound membrane as a temperature-response flexible electrode; taking the CuO/GO@PNIPAm compound membrane as a self-support working electrode, a saturatedcalomel electrode as a reference electrode and a platinum electrode as an auxiliary electrode to structure the glucose electrochemical sensor. The glucose electrochemical sensor is applied to testingthe state of glucose molecules. The method for preparing the CuO/rGO@PNIPAm compound self-support electrode and structuring the thermosensitive non-enzyme glucose sensor has the outstanding advantages of being high in compounding liability, mild and low in energy consumption. The prepared CuO/rGO@PNIPAm compound self-support flexible electrode can be applied to non-enzyme glucose detection and achieve significant temperature switch effects.