A kind of colloidal gold test strip and kit for detecting Clostridium difficile

Abstract

The present disclosure provides a colloidal gold test strip for detecting Clostridium difficile, the colloidal gold test strip comprises a water-absorbing pad, a base film, a gold standard pad and a sample pad connected in sequence; the base film is provided with C line and T line, wherein, the dCas9 antibody is coated on the C line, and the tcdB gene fragment-bovine serum albumin complex is coated on the T line; the dCas9 labeled with colloidal gold is contained in the gold label pad ‑gRNA-protein-nucleic acid complex. The present disclosure provides a kit for detecting Clostridium difficile, wherein the kit includes the above-mentioned colloidal gold test strip for detecting Clostridium difficile. Through the above technical solution, the present disclosure replaces the commonly used colloidal gold detection developed by antigen-antibody combination with the specific recognition of dCas9-gRNA and DNA, which effectively improves the detection specificity and reaction sensitivity.

Description

technical field [0001] The disclosure relates to the field of biomedicine, in particular to a colloidal gold test strip and a kit for detecting Clostridium difficile. Background technique [0002] Clostridium difficile is one of the major pathogens causing enteric infections in hospitalized patients and is a drug-resistant bacterium that can cause fatal infections in hospitals and nursing facilities. The U.S. Centers for Disease Control and Prevention listed it as the top of the list of "the most urgent drug resistance threat in the United States". Clinically, about 30% of antibacterial drug-associated diarrhea (antibiotic associated Chronic colitis and 95% to 100% of pseudomembranous colitis (pseudomembranouscolitis, PMC) are caused by Clostridium difficile infection (CDI). CDI is mainly caused by the overgrowth of toxin-producing Clostridium difficile, which leads to the imbalance of intestinal flora and the release of toxins. The main clinical symptoms are fever, abdomin...

AI Technical Summary

Problems solved by technology

CCTA and TC are currently the "gold standard" for detection of CDI, but they are difficult to be used in routine clinical detection of Clostridium difficile due to harsh culture conditions, high technical requirements, complex and time-consuming operations

EIAs detection is specific and fast, but the sensitivity is poor; GDH is relatively fast, but this method has cross-reactions, and some non-toxin Clostridium difficile can also secrete GDH, so false positives have occurred, the detection cost is high, and batch detection is required to reduce personnel costs. Operation and reagent costs, but at the same time prolong the detection cycle

The widely used PCR detection method at home and abroad is not suitable for primary hospitals and on-site rapid detection requirements due to high requirements for experimental equipment, cumbersome operation, and time-consuming.

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