A method for in vitro detection of allergens in food
An in vitro detection and allergen technology, which is applied in the direction of material inspection products, tension measurement, measuring devices, etc., can solve the problems of inaccurate reflection of allergen sensitization, etc., and achieve the effect of shortening the test cycle, short time and low detection cost
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Embodiment 17-8
[0045] Example 1 Detection of stimulation by 1.0 mg / ml 11S glycinin in female Hartley guinea pigs aged 7-8 weeks
[0046] 1) Experimental animals Female Hartley guinea pigs aged 7-8 weeks.
[0047] 2) Test substance The test protein is 11S glycinin.
[0048] 3) Dose The test protein dose is 1.0 mg / ml.
[0049] 4) Animal handling
[0050] The experimental animals were subjected to the experiment after 3 days of adaptive feeding. Water and food were given ad libitum.
[0051] Cut off at 1cm from the ileocecal part, take out a section of ileum about 10cm, place it in an oxygen-saturated Tyrode's solution culture dish, remove the mesentery along the intestinal wall, then cut the ileum into several small 1.5cm segments, and suck up Tyrode's solution with a syringe Flush the intestinal contents.
[0052] Take a small section of intestinal tube and place it in a petri dish filled with Tyrode's solution, tie a knot with thread at both ends of the intestinal tube, pay attention to...
Embodiment 2
[0055] Example 2 Detection of 5-6 week-old male Hartley guinea pigs stimulated by 0.1mg / ml 11S glycinin
[0056] 1) Experimental animals Male Hartley guinea pigs aged 5-6 weeks.
[0057] 2) Test substance The test protein is 11S glycinin.
[0058] 3) Dose The test protein dose is 0.1 mg / ml.
[0059] 4) Animal handling
[0060] The experimental animals were subjected to the experiment after 3 days of adaptive feeding. Water and food were given ad libitum.
[0061] Cut off at 1cm from the ileocecal part, take out a section of ileum about 10cm, place it in an oxygen-saturated Tyrode's solution culture dish, remove the mesentery along the intestinal wall, then cut the ileum into several small 1.5cm segments, and suck up Tyrode's solution with a syringe Flush the intestinal contents.
[0062] Take a small section of intestinal tube and place it in a petri dish filled with Tyrode's solution, tie a knot with thread at both ends of the intestinal tube, pay attention to keep the i...
Embodiment 3
[0065] Example 3 Detection of 0.1 mg / ml ovalbumin challenge in female Hartley guinea pigs aged 3-4 weeks.
[0066] 1) Experimental animals 3-4 weeks old female Hartley guinea pigs.
[0067] 2) Test substance The test protein is ovalbumin.
[0068] 3) Dose The test protein dose is 0.1 mg / ml.
[0069] 4) Animal handling
[0070] The experimental animals were subjected to the experiment after 3 days of adaptive feeding. Water and food were given ad libitum.
[0071] Cut off at 1cm from the ileocecal part, take out a section of ileum about 10cm, place it in an oxygen-saturated Tyrode's solution culture dish, remove the mesentery along the intestinal wall, then cut the ileum into several small 1.5cm segments, and suck up Tyrode's solution with a syringe Flush the intestinal contents.
[0072] Take a small section of intestinal tube and place it in a petri dish filled with Tyrode's solution, tie a knot with thread at both ends of the intestinal tube, pay attention to keep the i...
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