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Composition for memory improvement comprising petasites japonicus leaf extract

An extract and memory technology, applied in the field of memory-improving compositions, can solve the problems of economic benefit decline, low yield of active ingredients, liver toxicity, etc., and achieve excellent protection and regeneration effects

Active Publication Date: 2018-08-31
NATUREBIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the studies on memory improvement, the main extracts from crude drugs and other natural products or compound extracts were used. Animal experiments showed that part of the cognitive ability was improved, but it was limited to a negligible level of improvement and depended on a clear mechanism or There are many cases of experimental results where the site of action is unknown, and there is a problem of toxicity to the liver when a single extract using crude drugs or a complex extract based on oriental medicine prescriptions is involved.
And, the existing research for preparing the butterbur extract utilizes the method of performing concentration under reduced pressure for solvent removal in each process due to extraction processes including continuous use of ethanol, hexane, butanol as organic solvents. method, but this process takes a long time and has a high possibility of mistakes in the complicated process, and because chemical substances harmful to human body such as hexane and butanol are used in the extraction process, not only additional extraction facilities are required, And the yield of active ingredient is as low as about 1%, therefore has the shortcoming that economic benefit declines

Method used

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  • Composition for memory improvement comprising petasites japonicus leaf extract
  • Composition for memory improvement comprising petasites japonicus leaf extract
  • Composition for memory improvement comprising petasites japonicus leaf extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Confirmation of preparation, cytotoxicity and nerve cell protection effect of butterbur leaf extract (KP-1)

[0034] After extracting by adding 20 times of water to 1 kg of butterbur leaves, the extract was freeze-dried to obtain 250 g of a freeze-dried product (25% relative to butterbur leaves). Then, 70% ethanol was added to the water-extracted freeze-dried product of butterbur leaves, and eluted at room temperature for 24 hours. After 24 hours, the supernatant that dissolved unnecessary substances was removed, and only the precipitate of butterbur leaf extract was recovered, and 150 g of freeze-dried products were obtained by freeze-drying (relative to water extraction freeze-drying product is 60%). The final yield was 15% with respect to the butterbur leaves used, and 60% with respect to the water-extracted freeze-dried product of the butterbur leaves. As shown in Table 1 below, samples were prepared by the extraction method of butterbur leaves and cytotoxicity an...

Embodiment 2

[0039] Confirmed active ingredients of butterbur leaf extract (KP-1)

[0040] In order to explore the effective components of KP-1, after separating KP-1 into F1-F8 with resin DIAION HP-20, the F4 showing the highest cell survival rate was named KP-1F4 ( Figure 4 ). KP-1F4 was fractionated a second time to f1-f11 with SEPHADEX LH2. As a result, f1 and f2 had no activity in the pre-experiment, and in f9 and f11, a cell viability rate of more than 75% was shown ( Figure 5 ). The effective components of f9 and f11 were purified by liquid chromatography-mass spectrometry (LC-MASS) and nuclear magnetic resonance (NMR). It was confirmed that the components of f9 are the following chemical formulas 1 to 3, and the components of f11 are the following chemical formula 4.

[0041] Chemical formula 1:

[0042] 3,5-Di-O-caffeoylquinic acid

[0043] Chemical formula 2:

[0044] 4,5-Di-O-caffeoylquinic acid

[0045] Chemical formula 3:

[0046] 3,4-Di-O-caffeoylquinic acid ...

Embodiment 3

[0050] The memory-improving effect of butterbur leaf extract was confirmed

[0051] ICR (male, 7-week-old) was acclimatized and bred for one week for use. The breeding environment of this experiment was set at a temperature of 20-24°C, a relative humidity of 50-60%, a ventilation rate of 15 times per hour, and a light and dark lighting time of 12 hours (07:00-19:00). The temperature is 150-200Lux and raised here, so that the ICR can freely ingest solid feed and water for mice. Breeding was carried out in accordance with the Kyung Hee University animal husbandry regulations in compliance with the standards (No. 86-23, revised 1985) proposed by the National Institutes of Health (NIH, USA).

[0052] A passive avoidance test was performed to confirm the memory-improving effect of butterbur leaf extract in an Aβ-induced Alzheimer's type dementia model. The device for administering electric shocks was used in the dark by taking advantage of the light-to-dark habit of mice. The bo...

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Abstract

The present invention relates to a method for producing Petasites japonicus leaf extract effective for memory improvement, and to a composition for memory improvement comprising the Petasites japonicus leaf extract. The Petasites japonicus leaf extract obtained by the production method of the present invention has an excellent effect on nerve cell protection and regeneration.

Description

technical field [0001] The present invention relates to a preparation method of a butterbur leaf extract having a memory-improving effect and a composition for improving memory comprising the butterbur leaf extract. Background technique [0002] In the studies on memory improvement, the main extracts from crude drugs and other natural products or compound extracts were used. Animal experiments showed that part of the cognitive ability was improved, but it was limited to a negligible level of improvement and depended on a clear mechanism or There are many cases of experimental results where the site of action is unknown, and single extracts using crude drugs or complex extracts based on oriental medicine prescriptions contain actual toxicity, and there is a problem of liver toxicity when ingested in large amounts. And, the existing research for preparing the butterbur extract utilizes the method of performing concentration under reduced pressure for solvent removal in each pr...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61K31/216A23L33/105A23L33/10A61K9/00A61K9/08A61K9/20
CPCA61K9/00A61K9/08A61K9/20A61K31/216A61K36/28A23L33/10A23L33/105A23V2002/00A23V2200/322A61K9/0021A61K9/0043
Inventor 吴明淑金民山禹炅完朴相秀
Owner NATUREBIO CO LTD
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