Cell-substrate impedance monitoring of cancer cells
A cell matrix and cancer cell technology, which is applied in the field of evaluating the effectiveness of cancer cell anti-cancer therapy based on cell matrix impedance devices, and can solve the problem of difficulty in predicting the effectiveness of treatment methods.
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Embodiment 1
[0270] Overview of using cell matrix impedance monitoring to determine the response of cancer cells to anticancer drugs
[0271] In this study, we used cell matrix impedance monitoring (RT-CES system, ACEA Biosciences, Inc, San Diego, CA) to dynamically monitor the response of cancer cells to chemotherapeutic compounds that have been characterized by mechanisms and draw specific cell response maps. A total of 13 cancer cell lines, including breast, prostate, lung, colon, ovarian, kidney, fibroblast, and central nervous system cancers were tested (Table 1). Each cancer cell type is treated with 11 chemotherapeutic compounds. These compounds are classified as DNA damaging agents, protein kinase inhibitors, antimitotic drugs, cell cycle specific inhibitors, protein synthesis inhibitors, and an unknown compound ( Table 2). All test cell lines and compounds were characterized and summarized in dynamic and dose-dependent cell-compound interactions. Figures 9-11 show the general situa...
Embodiment 2
[0279] Cytotoxicity description
[0280] method
[0281] cell. All cells used in this study are from ATCC and maintained at 37°C, 5% CO 2 In a saturated incubator. The H460, HepG2 and HT1080 cells were maintained in RPMI medium containing 5% FBS and 1% penicillin and streptomycin. NIH3T3 cells were maintained in DMEM medium containing 10% FBS, 1% penicillin and streptomycin.
[0282] Cell proliferation detection. For each cell type, the 96X microtiter plate (microelectrode plate TM Each well of) is seeded with the indicated number of cells, and each well device contains a built-in electrode structure in 100 μL of culture medium. The RT-CES system (cell matrix impedance monitoring system) is used to continuously monitor cell adhesion, diffusion and proliferation every 30 minutes. According to the experiment, monitor cell proliferation for 48-72 hours. Electronic readings and cell sensor impedance are displayed as cell index parameters.
[0283] Drug handling and cytotoxicity eva...
Embodiment 3
[0287] Real-time monitoring of cancer cell proliferation using RT-CES
[0288] In order to evaluate the dynamic cell proliferation by monitoring the cell matrix impedance using the RT-CES system, H460 human lung cancer cells, H1080 fibrosarcoma cells, HepG2 human liver sarcoma cells and NIH3T3 mouse fibroblasts were placed on ACEA 96X microelectrode plates TM 2500 and 10,000 cells were inoculated into each well of, and each group repeated three wells. The RT-CES system was used to continuously monitor cells every 30 minutes during the indicated time period ( Figure 18 ). Such as Figure 18 As shown, each cell type has its own characteristic kinetic trajectory, which depends on the number of seeded cells, the overall size and morphology of the cells, and the degree of interaction between the cells and the sensor surface. In addition, adhesion and diffusion kinetics and the time for cells to enter the logarithmic growth phase are characteristics of each cell line shown. Therefore...
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