Fusion protein as well as preparation method and application thereof

A technology of fusion protein and protein, which is applied in the preparation, application in the field of tumor immunotherapy, and in the field of fusion protein, can solve the problem that the effect does not significantly improve the prognosis of patients, and achieve the effect of single component, cost reduction and clear structure

Active Publication Date: 2018-09-04
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main treatment for cervical cancer is surgery, and adjuvant chemotherapy is used for middle- and high-risk patients. For patients with advanced cervical cancer, radiotherapy is mainly used alone or in combination, but the effect does not significantly improve the prognosis of patients.

Method used

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  • Fusion protein as well as preparation method and application thereof
  • Fusion protein as well as preparation method and application thereof
  • Fusion protein as well as preparation method and application thereof

Examples

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Embodiment 1

[0047] Example 1 Construction and expression of purified fusion protein

[0048] 1. Sequence confirmation and cloning of Hsp70 and FPR1

[0049] The Hsp70 and FPR1 gene sequences were retrieved and analyzed in Gene Bank to obtain the nucleotide and amino acid sequences of the Hsp70 and FPR1 genes. Wherein, the amino acid sequence of Hsp70 is shown in SEQ ID NO.1. The complete amino acid sequence of FPR1 is shown in SEQ ID NO.2.

[0050] Since FPR1 is a seven-transmembrane protein, this application obtained four extracellular segments of FPR1 through analysis, and the analysis results are shown in Figure 1. The specific sequence of the FPR1 protein is as follows, wherein the part with the outer frame is the extracellular segment of FPR1.

[0051]

[0052]

[0053] In order to reduce irrelevant antigenic epitopes, the present application fused the extracellular segment of FPR1 protein with Hsp70 protein. First, the eukaryotic expression and purification system was used...

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Abstract

The invention belongs to the immunotherapy and discloses a fusion protein as well as a preparation method and application thereof. The fusion protein disclosed by the invention comprises a protein HSP70 and a protein fused with an extracellular domain protein FPR1. The extracellular domain protein FPR1 comprises multiple amino acid sequences, and the amino acid sequences of different fragments ofthe extracellular domain protein FPR1 are connected by flexible linker peptides. The fusion protein disclosed by the invention is simple in preparation, high in specificity and long in duration time,and the immunotherapy effect can be obviously enhanced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fusion protein, a preparation method and an application, especially an application in the field of tumor immunotherapy. Background technique [0002] Cervical cancer refers to malignant tumors that occur at the junction of squamous epithelial cells of the cervicovaginal or transition zone and columnar epithelial cells of the endocervical canal. Cervical cancer is the most common female reproductive system malignancy, the incidence of cervical cancer is second only to breast cancer. At present, the main treatment method for cervical cancer is surgery, and adjuvant chemotherapy is used for middle- and high-risk patients. For advanced cervical cancer patients, radiotherapy alone or in combination is mainly used, but the effect does not significantly improve the prognosis of patients. Therefore, finding effective therapeutic targets and methods is still a difficult point i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N15/85A61K39/385A61K39/00A61P35/00
CPCA61K39/0005A61K39/385A61P35/00C12N15/62C12N15/85C07K14/723A61K2039/6043C07K2319/35C07K2319/00C12N2800/107A61K39/4644A61K2239/31A61K39/4615A61K2239/38A61K39/4622A61K39/4611A61K2039/892C07K2319/32C07K14/47C07K14/705
Inventor 曹广明张震宇
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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