Collagen sponge and preparation method thereof

A technology of collagen sponge and collagen, which is applied in the fields of medical formula, application, and medical science, can solve the problems of toxicity, carcinogenicity and irritation of chemical cross-linking agents, achieve excellent hemostatic effect, simple preparation process, and ensure safety and the effect of effectiveness

Inactive Publication Date: 2018-09-07
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the products on the market are prepared by cross-linking with chemical cross-linking agents, which are toxic, carcinogenic and irritating

Method used

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  • Collagen sponge and preparation method thereof
  • Collagen sponge and preparation method thereof
  • Collagen sponge and preparation method thereof

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preparation example Construction

[0032] The invention relates to a preparation method of a collagen sponge, which is prepared by using collagen as a main raw material and adopting a combination method of enzyme low-temperature cross-linking and vacuum freeze-drying. The use of enzyme cross-linking technology abandons the toxicity, carcinogenicity and irritation of chemical cross-linking agents, ensures the application safety of the product, and improves the biocompatibility of the product. At the same time, the degradation characteristics of the product are improved, so that it can be well adhered to the wound, fully play the hemostatic effect, and ensure the effectiveness of the product. This method is specifically realized by the following steps:

[0033] Step 1: Prepare a collagen aqueous solution with a mass fraction of 1%-10% with deionized water;

[0034] Step 2: Add transglutaminase at a ratio of 2-20U (enzyme) / g (protein), and stir until dissolved;

[0035] Step 3: adjust the pH value to between 6-7...

Embodiment 1

[0044] Step 1: preparing an aqueous collagen solution with a mass fraction of 1% with deionized water;

[0045] Step 2: Add transglutaminase at a ratio of 20U (enzyme) / g (protein), and stir until dissolved;

[0046] Step 3: adjust the pH value to 6;

[0047] Step 4: quickly transfer the mixture obtained in step 3 to the mould;

[0048] Step 5: Place the mold at 4°C for 48 hours of cross-linking;

[0049] Step 6: Transfer the mold to -20°C for 2 hours, transfer to -80°C for 6 hours, and then dry it in a vacuum freeze dryer for 12 hours;

[0050] Step 7: Packaging and irradiation sterilization to obtain the finished product.

[0051] In step 1, the collagen is full-length collagen of animal origin.

[0052] In step 2, transglutaminase is an enzyme derived from mammalian tissues.

[0053] The pH regulator in step 3 is sodium hydroxide solution or hydrochloric acid solution, and the molar concentration is 0.1mol / L.

Embodiment 2

[0055] Step 1: preparing a collagen aqueous solution with a mass fraction of 5% with deionized water;

[0056] Step 2: Add transglutaminase at a ratio of 10U (enzyme) / g (protein), and stir until dissolved;

[0057] Step 3: adjust the pH value to 6.5;

[0058] Step 4: quickly transfer the mixture obtained in step 3 to the mould;

[0059] Step 5: Place the mold at 4°C for 36 hours of cross-linking;

[0060] Step 6: Transfer the mold to -20°C for 3 hours, transfer to -80°C for 4.5 hours, and then dry it in a vacuum freeze dryer for 36 hours;

[0061] Step 7: Packaging and irradiation sterilization to obtain the finished product.

[0062] In the first step, the collagen is animal-derived collagen polypeptide.

[0063] In step 2, transglutaminase is an enzyme derived from mammalian tissues.

[0064] The pH regulator in step 3 is sodium hydroxide solution or hydrochloric acid solution, and the molar concentration is 0.5mol / L.

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Abstract

The invention relates to collagen sponge and a preparation method thereof. The collagen sponge is prepared by combination of enzyme low temperature crosslinking and vacuum freeze-drying with collagenas a main raw material. The prepared collagen sponge adopts an enzyme as a crosslinking agent, the degradation characteristics of the product are improved, the collagen sponge can adhere to wounds well, a hemostatic effect is fully exerted, and the safety and effectiveness of the product are ensured.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a collagen sponge and a preparation method thereof. Background technique [0002] In wound first aid and surgical operations, wound bleeding and oozing are often encountered problems, and a large amount of blood loss can be life-threatening. At the same time, bleeding or oozing can also increase the chance of wound infection, thereby reducing the success rate of the operation. Therefore, a good hemostasis technique becomes the key to the success of the operation and is one of the cores of the operation technique. Degradable materials are widely used clinically as hemostatic materials. [0003] Collagen can promote wound healing, granulation growth and hemostasis. As a natural hemostatic agent, it not only has a strong affinity with cells, but also has a special affinity with growth factors that play a key role in the wound healing process. Collagen also prevents ...

Claims

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Application Information

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IPC IPC(8): A61L24/00A61L24/10
CPCA61L24/0036A61L24/0042A61L24/102A61L2400/04
Inventor 范代娣马晓轩严建亚段志广贾利平杨婵媛刘琳
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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