Polypeptide 8496 promoting platelet aggregation

A technology for platelet aggregation and polypeptide synthesis, which is applied in the field of medicine and biology, and can solve the problems of low response and single drug action mechanism.

Active Publication Date: 2018-09-07
FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] However, the existing antiplatelet drugs not only have side effects such as bleeding tendency (cerebral hemorrhage, gastrointestinal hemorrhage) [21], but also have resistance or low response in clinical application, and adverse ischemic events still occur; at the same time, the effects of these drugs The mechanism is single, and the antiplatelet effect has certain limitations: for example, aspirin irreversibly blocks the synthesis of COX-1, thereby inhibiting

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  • Polypeptide 8496 promoting platelet aggregation
  • Polypeptide 8496 promoting platelet aggregation
  • Polypeptide 8496 promoting platelet aggregation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0029] Example: Polypeptide 8496 human body provided by the present invention promotes collagen-induced human platelet aggregation

[0030] The effect of 8496 on platelet aggregation induced by arachidonic acid, thrombin, ADP and collagen was evaluated by optical turbidimetry.

[0031] Apheresis platelets were collected from healthy volunteers provided by the blood bank of our hospital.

[0032] (1) Dilute the peptide with deionized water containing 15% DMSO, and adjust the final concentration of the peptide to 10 mM; add an equal volume of 15% DMSO-free peptide-free deionized water to the control experimental group.

[0033] (2) Take 100 μl apheresis platelets and use 300 μl modified Tyrode’s buffer (137mM NaCl, 27mMKCl, 1 mM MgCl 2 , 0.42 mM NaH 2 PO 4 , 5.5 mM Glucose, 5.55 mM HEPES, 0.25% BovineSerum Albumin , pH 7.4) to dilute platelets, that is, platelet-rich plasma, and adjust the number of platelets to 2.5×10 8platelets / mL; take another 100 μl apheresis platelets a...

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Abstract

The invention discloses polypeptide 8496 promoting platelet aggregation, belongs to the field of medical biotechnology, and particularly relates to a polypeptide having a function of promoting the platelet aggregation. The preparation is the polypeptide 8496 having thrombin-induced human platelet aggregation, the sequence is Ile-Gly-Thr-Gln-Gln-Ala-Thr-Pro-Gly-Pro-Ala-Asn-Ser, the substance of thesynthesized polypeptide is a complete amino acid sequence. The polypeptide has a significant promoting effect on collagen-induced platelet aggregation, can be used for developing a new generation ofclinically-effective platelet aggregation promoting and hemostatic drugs, and can be used for exploring the influence of the polypeptide 8496 and other platelet activators on the effect and related functions of platelets.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to a polypeptide capable of promoting platelet aggregation. Background technique [0002] Cardiovascular and cerebrovascular diseases mainly include acute myocardial infarction, cerebral infarction, pulmonary infarction, ischemic heart disease, stroke and other common diseases, which seriously threaten human health and life and are the first cause of death among Chinese residents [1]. Platelet activation is an important initiating factor in the occurrence and development of cardiovascular and cerebrovascular diseases, and platelet activation, adhesion and aggregation to thrombus formation are a series of cascade reactions, which not only play a key role in the physiological hemostasis process, but also Participate in pathological processes such as thrombosis and tissue repair[2,3]. Platelet aggregation plays a central role in thrombus formation. Inducers o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P7/04A61P9/00A61P9/10A61P17/02A61P17/00
CPCA61K38/00C07K7/08
Inventor 孟照辉叶雨佳王华炜万雯王静
Owner FIRST AFFILIATED HOSPITAL OF KUNMING MEDICAL UNIV
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