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Preparation method for nanofiber membrane used for detecting ethyl carbamate

A technology of urethane and nanofiber membranes, applied in fiber treatment, biochemical fiber treatment, fiber chemical characteristics, etc., can solve the problems of long detection time, high professional technical requirements for testing personnel, and expensive equipment

Active Publication Date: 2018-09-07
南京宁高晶测生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the equipment required by gas chromatography-mass spectrometry is expensive, and the sample pretreatment is relatively complicated, the professional technical requirements of the testing personnel are high, and the detection takes a long time, so it is difficult to apply to the control of EC content in large quantities and multiple batches of alcoholic drinks

Method used

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  • Preparation method for nanofiber membrane used for detecting ethyl carbamate
  • Preparation method for nanofiber membrane used for detecting ethyl carbamate
  • Preparation method for nanofiber membrane used for detecting ethyl carbamate

Examples

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preparation example Construction

[0044] refer to figure 2 As shown in the workflow diagram, for simple, fast and accurate detection of the EC content in the sample, the application provides a method for preparing a nanofiber membrane for detecting the content of urethane, comprising the following steps:

[0045] Step 101, dissolving the spinning raw material in a solvent and mixing them uniformly to obtain a spinning stock solution, the mass concentration of the spinning stock solution is 5-20 wt / %.

[0046] During the electrospinning process, the viscosity of the spinning solution is an important factor affecting the morphology of the nanofibrous membrane. In a certain viscosity range, the viscosity of the spinning solution is high, and it is easy to form smooth and continuous nanofibers, but if the viscosity of the spinning solution is too high, the fluidity of the spinning solution will decrease, and the electric field pulling force is smaller than the viscosity of the spinning solution. , it is difficul...

Embodiment 1

[0093] 1) Preparation of spinning solution

[0094] Select medical grade polyvinyl alcohol (polyvinyl alcohol, PVA) as the spinning raw material, weigh 20g of medical grade PVA, dissolve in 80g of deionized water, stir in a water bath at 90°C for 30min, and prepare a spinning fiber with a mass concentration of 20wt%. Silk stock solution.

[0095] Take 0.1 g of 1000 U / mg acetylcholinesterase, dissolve it in 100 mL of phosphate buffered saline (Phosphate Buffered Saline, PBS), and prepare an enzyme preparation with a concentration of 1 mg / mL. The pH of the PBS solution used in the examples of this application is 7.4.

[0096] 0.5 g of the color-developing composition and 100 mL of PBS solution were prepared into a color-developing agent with a concentration of 5 mg / mL.

[0097] Take 10 mL of the prepared spinning stock solution and 10 mL of the enzyme preparation, mix and stir at 37° C. for 20 min to obtain the spinning solution A.

[0098] Take 10 mL of the prepared spinning ...

Embodiment 2

[0102] 1) Preparation of spinning solution

[0103] Select medical-grade hydroxycellulose (Hydroxyethyl Cellulose, HEC) as spinning raw material, weigh 5g of medical-grade HEC, dissolve in 95g of deionized water, stir in a water bath at 90°C for 30min, and prepare a spinning fiber with a mass concentration of 5wt%. Silk stock solution.

[0104] Take 0.02 g of 1000 U / mg acetylcholinesterase, dissolve it in 100 mL PBS solution, and prepare an enzyme preparation with a concentration of 0.2 mg / mL. The pH of the PBS solution used in the examples of this application is 7.4.

[0105] 0.02 g of the color-developing composition and 100 mL of PBS solution were prepared to form a color-developing agent with a concentration of 0.2 mg / mL.

[0106] Take 10 mL of the prepared spinning stock solution and 10 mL of the enzyme preparation, mix and stir at 37° C. for 20 min to obtain the spinning solution A.

[0107] Take 10 mL of the prepared spinning stock solution and 5 mL of the color devel...

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Abstract

The invention provides a preparation method for a nanofiber membrane used for detecting ethyl carbamate. The preparation method comprises the steps that spinning materials are dissolved in solvent andmixed uniformly to obtain a spinning solution; acetylcholin esterase and a phosphate buffer are mixed to prepare an enzymic preparation; parts of spinning solution and the enzymic preparation are mixed according to the volume ratio that the spinning solution volume to the enzymic preparation volume is (1-2):1, and a spinning solution A is obtained; another parts of spinning solution and a color developing agent are mixed according to the volume ratio that the spinning solution volume to the color developing agent volume is (2-3):1, and a spinning solution B is obtained; the spinning solutionA and the spinning solution B are subjected to electrostatic spinning to obtain the nanofiber membrane. When the nanofiber membrane is utilized to detect ethyl carbamate, the contact area between theethyl carbamate and nanofibers is larger, so that the ethyl carbamate, the color developing agent and the enzymic preparation take effect, thereby generating a color developing result, and the color developing result is utilized to quickly and accurately judge the ethyl carbamate content in a sample.

Description

technical field [0001] The present application relates to the technical field of nanofiber membrane preparation, in particular to a method for preparing a nanofiber membrane used for detecting the content of urethane. Background technique [0002] Ethyl Carbamate (EC) is also known as urethane, urethane, etc., and its molecular formula is C3H7NO2. EC, a naturally occurring contaminant of fermented foods and alcoholic beverages during fermentation or storage, is a multisite carcinogen that can cause lung, lymphoma, liver, and skin cancers in rodents. Because ethanol can promote the carcinogenicity of EC, and alcoholic beverages contain ethanol, alcoholic beverages with high EC content are more harmful. [0003] The international standard content of urethane in food is 20μg / L. In order to ensure that the content of urethane in food is lower than the international standard, it is necessary to detect the EC content in food, especially in alcoholic beverages. At present, the de...

Claims

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Application Information

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IPC IPC(8): D04H1/4382D04H1/728D06B3/10D01F6/34D01F1/10D01F2/00D01F6/92D06M16/00
CPCD01F1/10D01F2/00D01F6/34D01F6/92D04H1/4382D04H1/728D06B3/10D06M16/003
Inventor 王陈苗红
Owner 南京宁高晶测生物科技有限公司
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