Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Self-luminous tobacco and genetic modification method

A luminescent gene, self-luminous technology, applied in horticultural methods, genetic engineering, botanical equipment and methods, etc., can solve the problems of inconspicuous plant luminescence, lack of luminous tobacco, low transformation rate, etc., to achieve safe plant growth, The effect of constant luminous intensity for easy observation

Pending Publication Date: 2018-09-14
云南纳博生物科技有限公司
View PDF9 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The luminescent gene is integrated into the plant chromosomal gene by the Agrobacterium transformation method, the copy number is low, resulting in a very low transformation rate, and the plant luminescence is not obvious. When this method is applied to tobacco, almost no luminescent tobacco can be obtained.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Self-luminous tobacco and genetic modification method
  • Self-luminous tobacco and genetic modification method
  • Self-luminous tobacco and genetic modification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Using Nicotiana benthamiana (diploid) as the recipient, the LUX gene was transferred into its chloroplast to develop self-luminous tobacco

[0046] Step (1) Search and obtain the LUX gene sequence on NCBI, and synthesize the LUX gene in the carrier p-GH-LUX in Shanghai Jierui Bioengineering Co., Ltd. The nucleotide sequence can be found in NCBI), design IN-fusion connection primers, and amplify by PCR to obtain the target band. The PCR primers are as follows:

[0047] primer-F: AGGGAGGGATCCATGGGAATACCAAAGGAGATTAC;

[0048] primer-R: TAATGTCTACTCTAGAAATATCCCTATAAATAGAAC;

[0049] When the luminescent gene LUX is subjected to PCR amplification, the PCR reaction system (25ul) is shown in Table 1.

[0050] Table 1

[0051]

[0052] The PCR reaction conditions are shown in Table 2.

[0053] Table 2

[0054]

[0055] Perform gel electrophoresis after the PCR reaction results, and use 1.2% gel to recover the target fragment, such as figure 1 shown. figur...

Embodiment 2

[0145] Example 2 Using noble tobacco (tetraploid) as the recipient, the LUX gene was transferred into the air chloroplast genome to develop self-luminous tobacco

[0146] Step (1) The source and amplification system of the LUX gene are the same as in Example 1.

[0147] Step (2) The construction method of the p-LUX vector is the same as that in Example 1.

[0148] Step (3) import the p-LUX carrier into tobacco by means of "gene gun bombardment": the hyperosmotic medium and leaf hyperosmotic treatment methods are the same as in Example 1, and the sterilization treatment of gene gun accessories and the preparation of microcarriers are also the same as in Example 1 1. The only difference between the bombardment of leaves by the gene gun method and that of Example 1 is that the bombardment distance of Example 2 is only 6 cm, and the recovery medium formula and recovery culture method are the same as in Example 1.

[0149] The leaves after step (4) recovery culture are screened on...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a self-luminous tobacco and a genetic modification method. The self-luminous tobacco is prepared by constructing a luminous gene-plastid vector expression vector from a luminous gene and a plasmid vector, importing the luminous gene-plastid vector expression vector into tobacco leaves by a plastid genetic modification technology and performing tissue culture on the tobaccoleaves. The plasmid vector is a p-DK6 plasmid vector; a base sequence is shown as SEQ ID NO. (sequence identifier number) 1; and the luminous gene is a LUX gene. The self-luminous tobacco is obtainedby the plastid genetic modification technology; the method depends on homologous recombination of an exogenous gene and a chloroplast gene and has the advantages that the exogenous gene is subjected to fixed-point integration and inherited stably, the environmental safety is good, a gene product is regionalized, and an expression quantity is extremely high; and therefore, the method is favored byscientists.

Description

technical field [0001] The invention belongs to the technical field of transgenic luminous plants, and in particular relates to a self-luminous tobacco and a transgenic method. Background technique [0002] In the course of biological evolution, autofluorescent species of algae, fungi, bacteria, insects, etc. have been formed. However, there are few autofluorescent plants in nature. The research on luminous tobacco in the world involves all aspects. Most of the patents of luminous tobacco are to apply certain luminescent compounds on the surface of plants, or to modify the plant cultivation device to make plants glow in the dark. [0003] The vigorous development of genetic engineering has enabled scientists to study the mechanism of bioluminescence at the molecular level. Scientists obtain corresponding luminescent plants by introducing luminescent genes into the chromosomes of plant cells. In nature, animals and microorganisms can emit light, but only plants do not emit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H5/00C12N15/82A01H4/00A01H6/82
CPCA01H4/00C12N15/8207C12N15/8242
Inventor 段康张晨赵娟生承晔张业胜王文董扬
Owner 云南纳博生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com