Method for detecting descendant gene type before delivery and application

Abstract

The invention provides a method for detecting a descendant gene type antepartum and application. The method comprises the following steps: (1) selecting no less than 30 gene sites and calculating theminimum allele frequency of each gene site; (2) clustering sets of the minimum allele frequency in step (1) and dividing into four types; determining a clustering result of each gene site; (3) obtaining a gene type combination of the gene sites of parental generations and descendants according to the clustering result of the gene sites in step (2); (4) obtaining the gene types of the gene sites ofthe descendants according to the gene types of the gene sites of the parental generations. According to the method provided by the invention, a mathematics clustering thought is applied to the fieldof biology and the clustering result of the minimum allele frequency is determined so that the gene type of a fetus in gestation period is detected.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method and application for prenatal detection of offspring genotype. Background technique [0002] Deafness is an auditory dysfunction caused by lesions in the auditory system and the auditory nerve and central nervous system at all levels in the auditory system and auditory conduction pathway, which can cause hearing loss to varying degrees. Congenital deafness is one of the most common birth defects and the most common human sensory system disease, with an incidence rate of 0.1-0.3%. Among them, 60% of deafness is caused by genetic factors, and another 40% is related to environmental factors. Hereditary deafness is divided into comprehensive deafness and non-synthetic deafness. Non-synthetic deafness with deafness as the only symptom accounts for 70% of all hereditary deafness, and has a high degree of genetic heterogeneity, and comprehensive deafness accounts for 30%. [0003] C...

AI Technical Summary

Problems solved by technology

[0004] Although the newborn deafness gene detection technology has matured and been introduced to the market, the detection technology is suitable for newborn babies, which is a remedy for the results, and will still bring economic and mental burden to the society and families of newborn deaf children

Relying on amniocentesis technology can achieve accurate genetic testing during fetal life, but amniocentesis may lead to infection, miscarriage or neonatal respiratory distress, and there are certain risks

[0005] CN 107190064A discloses the SNaPshot kit for detecting the polymorphism of the deafness gene at 22 sites, which is helpful for early detection of children carrying the mutation of the deafness gene. However, the kit is only applicable to newborn babies and is a remedy for the result However, these measures will still bring economic and mental burdens to the society and families of newborn deaf children, and cannot achieve prenatal prediction

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