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pH monitoring flat panel optrode fluorescence sensing film, preparation method and application

A technology of fluorescent sensing film and optode, which is applied in the direction of fluorescence/phosphorescence, testing pH value, chemical instruments and methods, etc., can solve the problems of narrow application range and easy leakage of fluorescent dyes, etc., to reduce the possibility of dye leakage, The effect of strong hydrophobicity and enhanced hydrophobicity

Active Publication Date: 2018-09-25
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] Aiming at the problems of strong water solubility of HPTS, limited application in the natural environment, easy leakage of the fluorescent dye of the prepared pH fluorescent sensing film, and narrow application range, the present invention provides a new fluorescent dye and pH flat plate optode Fluorescence sensing film, preparation method and application

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  • pH monitoring flat panel optrode fluorescence sensing film, preparation method and application
  • pH monitoring flat panel optrode fluorescence sensing film, preparation method and application
  • pH monitoring flat panel optrode fluorescence sensing film, preparation method and application

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Embodiment 1

[0053] The preparation process of the fluorescent dye HPTS-lipo of the present embodiment comprises the following steps:

[0054] 1) In 2mol / L NaOH solution, HPTS and acetic anhydride were reacted at room temperature according to 1:3 times equivalent, and the product was extracted with absolute ethanol and suction filtered to obtain the hydroxyl-protected product;

[0055] 2) Hydroxyl-protected HPTS and thionyl chloride were heated to reflux at 90°C for 2 hours according to the ratio of 1:4 times to obtain the sulfonyl chloride intermediate, and the thionyl chloride was spin-dried to obtain HPTS-SO 2 Cl;

[0056] 3) HPTS-SO 2 Dissolve Cl in dichloromethane, add dropwise dimethylamine at the ratio of 1:3.1 equivalents under ice-bath conditions, spin evaporate and deprotect with 1mol / L NaOH after the reaction, and obtain the final product through column separation to obtain the pure product HPTS -lipo, dimethylamine-HPTS, figure 2 is a schematic diagram of the chemical struc...

Embodiment 2

[0075] The preparation process of the fluorescent dye HPTS-lipo of the present embodiment comprises the following steps:

[0076] 1) In 2mol / L NaOH solution, HPTS and acetic anhydride were reacted at room temperature according to the ratio of 1:3 times, and the product was extracted with absolute ethanol and suction filtered to obtain the hydroxyl-protected product;

[0077] 2) Hydroxyl-protected HPTS and thionyl chloride were heated to reflux at 90°C for 2 hours according to the ratio of 1:4 times to obtain the sulfonyl chloride intermediate, and the thionyl chloride was spin-dried to obtain HPTS-SO 2 Cl.

[0078] 3) HPTS-SO 2 Cl was dissolved in dichloromethane, and di-n-butylamine was added dropwise at an equivalent ratio of 1:3.1 under ice bath conditions. After the reaction was completed, it was rotary evaporated and deprotected with 1mol / L NaOH to obtain the final product, which was separated by column separation into pure The product HPTS-lipo, that is, di-n-butylamin...

Embodiment 3

[0090] The preparation process of the fluorescent dye HPTS-lipo of the present embodiment comprises the following steps:

[0091] 1) In 2mol / L NaOH solution, HPTS and acetic anhydride were reacted at room temperature according to the ratio of 1:3 times, and the product was extracted with absolute ethanol and suction filtered to obtain the hydroxyl-protected product;

[0092] 2) Hydroxyl-protected HPTS and thionyl chloride were heated to reflux at 90° C. for 2 hours to obtain a sulfonyl chloride intermediate; DCM was used as a solvent in an ice bath, and di-n-butylamine was added dropwise;

[0093] 3) Step 2) After the reaction is completed, spin dry and deprotect with 1 mol / L NaOH to obtain the final product through column separation to obtain the pure product HPTS-lipo.

[0094] The steps for preparing a two-dimensional pH fluorescent sensing membrane using the above-mentioned fluorescent dye HPTS-lipo are as follows:

[0095] 1) Dissolve 1 g of hydrogel D4 in 10 mL of 90% a...

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Abstract

The invention provides a pH monitoring flat panel optrode fluorescence sensing film, as well as a preparation method and application thereof, and belongs to the field of monitoring of pH value two-dimensional distribution. The fluorescence sensing film is prepared from a fluorescent dye HPTS-lipo. In the preparation process, HPTS-lipo is embedded in a hydrogel D4. The fluorescent dye HPTS-lipo isprepared in such a way that a sulfonic acid group of the fluorescent dye HPTS-lipo is introduced in alkylamine. Based on modified fluorescent dye HPTS-lipo, and compared with a fluorescent dye HPTS, the pKA value is obviously increased, the fluorescence sensing film is suitable for monitoring different pH values, different experimental requirements are met, the fluorescent dye HPTS-lipo has higherhydrophobicity, and is kept for a longer time in an environment, and after embedding by the hydrogel D4, the problem of dye leakage is further solved.

Description

technical field [0001] The invention belongs to the technical field of flat-plate optodes for monitoring two-dimensional pH value, and more specifically relates to a pH flat-plate optode fluorescent sensing film, a preparation method and an application thereof. Background technique [0002] In the field of environment, pH value is an important index affecting the geochemical cycle of various heavy metals and metalloid elements. Taking the rhizosphere as an example, plant roots can affect the pH value of the surrounding environment by secreting root exudates and other behaviors, causing acidification or alkalization of the rhizosphere. Therefore, the rhizosphere effect is very important for the study of the geochemical cycle of heavy metals and metalloids. important. [0003] At present, the traditional method for pH measurement is mainly based on electrode sensing. On the one hand, because the price is relatively expensive, and it is a single-point measurement, it will be m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09B57/00
CPCG01N21/6428C09B57/001G01N2021/6434G01N21/80G01N31/221G01N2021/7786G01N21/643
Inventor 罗军胡璇梁潮根方文刘兆东尹带霞
Owner NANJING UNIV
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