Method of extracting fish oil from squid skin
A technology for fish oil and cod, which is applied in the directions of fat oil/fat production and fat production, can solve problems such as high cost and low yield, and achieve the effects of high cost, low yield and high economic benefit.
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Embodiment 1
[0015] After crushing and drying 10 g of fish skin, move them all into the filter paper tube, put the filter paper tube into the extraction tube of the Soxhlet extractor, connect the receiving bottle that has been dried to constant weight, and add petroleum ether from the upper end of the condenser tube of the extractor To two-thirds of the volume of the bottle, heated in a water bath to continuously reflux the extraction solvent for 12 h. Remove the receiving bottle, recover petroleum ether, evaporate to dryness on a water bath when about 2 mL of solvent remains in the receiving bottle, dry at 100°C for 2 h, cool in a desiccator for 0.5 h, and weigh. Fish oil production is 0.64%. The relative content of oleic acid in fish oil is 29.88%, the content of EPA is 2.899%, and the content of DHA is 3.76%.
Embodiment 2
[0017] After crushing and drying 10 g of fish skin, put them all into the filter paper tube, put the filter paper tube into the extraction tube of the Soxhlet extractor, connect the receiving bottle that has been dried to constant weight, and add ether from the upper end of the condenser tube of the extractor to Two-thirds of the volume of the bottle was heated in a water bath to continuously reflux the extraction solvent for 12 h. Remove the receiving bottle, recover ether, evaporate to dryness on a water bath when about 2 mL of solvent remains in the receiving bottle, then dry at 100°C for 2 h, cool in a desiccator for 0.5 h, and weigh. Fish oil production is 1.21%. The relative content of oleic acid in fish oil is 18.38%, the content of EPA is 3.52%, and the content of DHA is 4.48%.
Embodiment 3
[0019] After crushing and drying 10 g of fish skin, move them all into the filter paper cylinder, put the filter paper cylinder into the extraction cylinder of the Soxhlet extractor, connect the receiving bottle that has been dried to constant weight, and add ethyl formate from the upper end of the condenser tube of the extractor. The ester was filled to two-thirds of the volume of the bottle, heated in a water bath to continuously reflux the extraction solvent for 12 h. Remove the receiving bottle, recover ethyl formate, evaporate to dryness on a water bath when about 2 mL of solvent remains in the receiving bottle, dry at 100°C for 2 h, cool in a desiccator for 0.5 h, and weigh. Fish oil yield is 0.95%. The relative content of oleic acid in fish oil is 21.82%, the content of EPA is 6.36%, and the content of DHA is 7.98%.
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