Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Selective culture medium for quantitative detection of pichia kudriavzevii and preparation method and application thereof

A technology for quantitative detection of Pichia pastoris, applied in the field of microbiology, can solve the problems of high cost, complicated process, and inability to detect the status of live yeast

Active Publication Date: 2018-09-28
KWEICHOW MOUTAI COMPANY
View PDF6 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the fermentation process of liquor, the method for the quantitative detection of yeast mainly includes two kinds: (1) apply the sample to be tested on non-selective medium (such as YPD medium), and then utilize molecular means to plate All the above yeasts are sequenced and identified to calculate the absolute number of specific types of yeasts. However, this method requires a large number of yeasts to be sequenced and identified. Therefore, the workload is large, the cost is high, and the cycle is long, which cannot meet the needs of rapid detection; ( 2) Quantitative analysis of a certain type of yeast in the sample is carried out by designing primers for specific yeast and using uncultured methods (such as fluorescent quantitative PCR). Although this method is fast and accurate, it requires special detection instruments, complicated procedures, and high detection costs. Moreover, this method can only detect the nucleic acid status of specific yeast, but cannot detect the live bacteria status of specific yeast

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Selective culture medium for quantitative detection of pichia kudriavzevii and preparation method and application thereof
  • Selective culture medium for quantitative detection of pichia kudriavzevii and preparation method and application thereof
  • Selective culture medium for quantitative detection of pichia kudriavzevii and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: The preparation method of the selective medium for the quantitative detection of Pichia pastoris

[0028] (1) Weigh 20g of peptone; 10g of yeast extract; MgSO 4 2.5g; NaCl 1.0g; FeSO 4 0.5g; agar 20g, add distilled water to dissolve to 1L to obtain a liquid medium with a natural pH of 2.90, and sterilize the liquid medium obtained above at 121°C for 15 minutes;

[0029] (2) After cooling the above-mentioned liquid culture medium after autoclaving to 50-60° C., add 40 g of lactic acid and 40 g of ethanol therein, then fully mix and distribute for use.

Embodiment 2

[0030] Example 2: Selective Medium for Quantitative Detection of Pichia pastoris Kudriaz

[0031] Table 1 Selective medium for the quantitative detection of Pichia wisteri

[0032]

Embodiment 3

[0036] Example 3: The detection rate of the selective medium for Pichia pastoris Kudriaz

[0037] A total of 13 media plates were prepared from the selective media A-M and non-selective YPD media G in Example 2, and 100 uL of liquid-cultured pure Pichia kudriazzia was coated on the above 13 media on a plate, and then cultured at a temperature of 30°C for 2-7 days, and the number of colonies was counted after the culture was completed.

[0038] Since the non-selective YPD medium is suitable for cultivating different microorganisms, the present invention takes the number of colonies in the non-selective YPD medium as 100% detection rate as a reference, and counts the effects of the above-mentioned 12 selective mediums on Kudry Aziwi The detection rate of Pichia pastoris, its calculation method is: Detection rate (%)=the total number of bacterial colonies in the selective medium / non-selective YPD medium The number of Pichia wisteria Kudriazzi × 100%, Specifically as shown in Tab...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a selective culture medium for quantitative detection of pichia kudriavzevii, and a preparation method and application thereof. Each liter of the selective culture medium comprises the following components: 20-40g of lactic acid; 20-40g of ethanol; 10-20g of peptone, 5-15g of yeast extract; 1-5g of MgSO4;0.5-1.5g of NaCl; 0.1-1g of FeSO4; 15-20g of agar, the balance being distilled water, and the natural pH is 3.0-0.5, as the selective medium is for the characteristics of the pichia kudriavzevii; wherein a high concentration of lactic acid and an amount of ethanol are added so that the pH of the selective medium is relatively low; the selective culture medium can effectively inhibit the growth of other microorganisms in the brewing process of the white spirit; the purpose of fast and accurate quantitative detection of the pichia kudriavzevii can be achieved by the selective culture medium.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a culture medium for quantitatively detecting Pichia wisteri and a preparation method thereof. Background technique [0002] The main components of Chinese liquor are ethanol and water (accounting for 98%-99% of the total), and the trace components accounting for 1%-2% of the total (referring to many kinds of esters, acids, alcohols, aldehydes, etc. Trace organic compounds) as the aroma and taste substances of liquor determine the style and quality of liquor. Among them, the esters in the trace components have aromatic flavor substances, which are the main factors for the strong aroma of the wine body. In the national standards of various flavor-type liquors, the total ester content is an important index for classifying liquor grades. For example, the total ester content of Maotai-flavor premium liquor should be higher than 2.2g / L, and the total ester content of Luz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/045C12Q1/06
Inventor 陈良强王莉万波汪地强王和玉杨帆吕锡斌
Owner KWEICHOW MOUTAI COMPANY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com