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Cinnamomum camphora-based biological algaecides and preparation methods thereof

A technology of algaecide and camphor, which is applied in the field of algaecide and environmental protection, can solve the problems of destroying the ecological environment, water pollution, poisoning aquatic organisms, etc., and achieve the effects of simple procurement, fast preparation process and convenient storage

Inactive Publication Date: 2018-10-02
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in eutrophic water bodies and small water bodies, algae (mainly cyanobacteria, occasionally green algae) multiply and grow in large numbers, thus causing water pollution, poisoning aquatic organisms, and destroying the ecological environment. Existing physical, chemical and biological methods There are many problems in the method of algae removal, which cannot be implemented in practice

Method used

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  • Cinnamomum camphora-based biological algaecides and preparation methods thereof
  • Cinnamomum camphora-based biological algaecides and preparation methods thereof
  • Cinnamomum camphora-based biological algaecides and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of described camphor methanol extract comprises the following steps:

[0022] (1) Clean and dry the camphor leaves, and grind the dried camphor leaves into powder;

[0023] (2) Add the obtained Cinnamomum camphora leaf powder into the methanol solution, and extract at room temperature and in the dark for 36-48 hours to obtain a Cinnamomum camphora methanol extract with an extract concentration of 4-5 mM.

[0024] In this example, according to the types of the main harmful algae cyanobacteria and green algae, the typical species of cyanobacteria blooms, Microcystis aeruginosa, and Chlamydomonas reinhardtii, a typical species of green algae, were respectively selected to carry out algae removal experiments. In the Chlamydomonas reinhardtii cell solution, apply different molar concentrations of camphor methanol extract, and record its algae removal effect in two time periods of 24h and 48h, see Table 1;

[0025]

[0026] It can be seen from Table...

Embodiment 2

[0028] The preparation method of described Cinnamomum camphora water extract comprises the following steps:

[0029] (a) clean the leaves of camphor camphor, dry them, and grind the dried leaves of camphor camphor into powder;

[0030] (b) Add the obtained Cinnamomum camphora leaf powder into pure water, and extract at room temperature and in the dark for 36-48 hours to obtain a Cinnamomum camphora water extract with an extract concentration of 1.5-2.5 mM.

[0031] In this example, according to the types of the existing main harmful algae cyanobacteria and green algae, the typical algal species of cyanobacteria blooms, Microcystis aeruginosa, and the typical algal species of green algae, Chlamydomonas reinhardtii, were selected to carry out algae removal experiments. Solution and Chlamydomonas reinhardtii cell solution, apply the camphor water extract of different molar concentrations, record its algae removal effect in two time periods of 24h and 48h, see Table 2;

[0032] ...

Embodiment 3

[0036] In this embodiment, the algae removal effect of camphor leaves is analyzed by GC-MS for its main components. The contents of camphor, terpineol and linalool are relatively high, which are the main components of the water extract and methanol extract of camphor leaves. Components, the molar concentration ratio is about 1:2.6~2.9:6.3~7.5, see Table 3;

[0037]

[0038] In this example, a mixed algae-removing solution is prepared according to the concentration ratios of the three main components in the camphor leaf extract (see Table 3), wherein the preparation method of the mixed algae-removing solution includes the following steps:

[0039] (A) Take camphor, terpineol, and linalool, add them to ethanol respectively, and mix them at a molar ratio of 1:2.6~2.9:6.3~7.5 to obtain the mixed algae removal solution. The concentration is 100 mM.

[0040] Camphor, terpineol, and linalool can be purchased or extracted from camphor leaves.

[0041] In this example, according t...

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Abstract

The invention discloses cinnamomum camphora-based biological algaecides and preparation methods thereof. Three different preparation methods of the algaecides are provided, and cinnamomum camphora methanol extract and cinnamomum camphora water extract which are extracted from cinnamomum camphora leaves as well as a mixed algaecide solution prepared from camphor, terpineol and linalool by compounding are obtained respectively. The algaecides prepared with the methods have different characteristics; the cinnamomum camphora methanol extract has outstanding killing effect on microcystis aeruginosaand chlamydomonas reinhardtii; the effect of the cinnamomum camphora water extract is slightly poorer than that of the methanol extract, but the cost is lower; the mixed algaecide solution adopts easily purchasable raw materials and rapid preparation process, is convenient to store, can be prepared in emergency, and can effectively kill both the microcystis aeruginosa and the chlamydomonas reinhardtii under higher molar concentration. Besides, the algaecides are eco-environmentally friendly because effective components are all from plants and can be naturally degraded.

Description

technical field [0001] The invention relates to the fields of algicide and environmental protection, in particular to a camphor-based biological algicide and a preparation method thereof. Background technique [0002] A large amount of domestic sewage and industrial wastewater rich in N and P are discharged into rivers and lakes, and then collected into the ocean, causing eutrophication of the water body, causing algae and other plankton to multiply rapidly and causing serious environmental disasters. It occurs frequently in various lakes, rivers and sea areas in my country. The massive reproduction of algae not only seriously damages the water environment, but also endangers the safety of the ecosystem. [0003] At present, in eutrophic water bodies and small water bodies, algae (mainly cyanobacteria, occasionally green algae) multiply and grow in large numbers, thus causing water pollution, poisoning aquatic organisms, and destroying the ecological environment. Existing ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/24A01N49/00A01N35/06A01N31/04A01P13/00
CPCA01N31/04A01N35/06A01N49/00A01N65/00A01N65/24
Inventor 左照江叶超琳郑铁枫乌兰·胡安尼西陈思兰祖木拉提·牙克甫
Owner ZHEJIANG FORESTRY UNIVERSITY
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