Method for measuring whey protein in dairy product on basis of data-driven Raman spectrum

Abstract

The invention relates to a method for measuring whey protein in a dairy product on the basis of data-driven Raman spectrum. According to the method, the optimal variable combination of a to-be-measured substance is accurately extracted from complicated and fluctuant translation invariant wavelet coefficients with a variable screening method, the variable combination is subjected to spectral reconstruction with the adoption of translation invariant wavelet transform, the best time domain/frequency domain resolution ratio is obtained while spectral interference such as matrixes and the like areeffectively stripped, and following modeling analysis is facilitated. Besides, according to the method, by analyzing reconstructed spectrum data features and inherent law of unknown dairy product samples, separate modeling is only performed on a single unknown dairy product sample, so that a target ideal model is dynamically approached, and the uncertainty of actual dairy product sample products is effectively overcome. Finally, a data-driven model of alpha-lactalbumin and beta-lactoglobulin in the dairy product sample is finally constructed and converted into corresponding whey protein content, nondestructive testing of the whey protein in the dairy product is further realized, and field detection of related dairy products can be conveniently performed.

Description

technical field [0001] The invention relates to the field of dairy component detection, in particular to a method for measuring whey protein in dairy products based on data-driven Raman spectroscopy. Background technique [0002] With the continuous deepening of research on whey protein, its application has become more and more extensive, and it has become the main raw material of infant milk powder and infant formula liquid milk. In my country's national standard GB10765-2010 "Infant Formula Food", it is required that "milk The content of whey protein in basic infant formula should be ≥ 60%", that is, in infant formula with milk or milk protein products as the main raw material, the proportion of whey protein in the total protein should be greater than or equal to 60%. However, there is no accurate quantitative method for whey protein at home and abroad. In view of the importance of the detection method, the Ministry of Health of my country entrusted the National Dairy Prod...

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Problems solved by technology

[0003] However, the operation process of high performance liquid chromatography-mass spectrometry is relatively cumbersome, and complex operations such as enzyme digestion, high-fold dilution, isotope labeling of peptides, and liquid phase separation are required, resulting in the stability and versatility of the method. At the same time, there are also practical difficulties in the determination of whey protein by liquid chromatography-mass spectrometry, such as the long time-consuming due to the complicated operation process, the high detection cost due to expensive instruments and the consumption of reagent consumables, and the The regulatory risk caused by the shortage of isotope peptide supply cannot meet the requirements of large-scale and efficient supervision

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