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A 128-channel array capillary electrophoresis instrument

A technology of capillary electrophoresis and array, which is applied in the direction of material analysis, instruments, and scientific instruments through electromagnetic means. It can solve the problems of many adjustment parts, laser irradiation to capillary, and increase the difficulty factor of instruments, so as to reduce manufacturing costs and use. cost, improve fluorescence collection efficiency, and reduce the effect of external light interference

Inactive Publication Date: 2021-04-13
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No. 200610048056.X patent application is based on the No. 03133511.X patent application, and the gas laser is used as the excitation light source, the sample injection method is single, the lack of electrophoresis high-voltage control system, and the gas laser has unstable performance and short life. Optimized
Because these two patents use a large number of dispersed independent optical elements, there are many parts that need to be adjusted, which greatly increases the difficulty of using the instrument, and it is impossible to accurately irradiate the laser on the capillary; moreover, the control of the ambient background light is strict. It needs to be completely protected from light; at the same time, it is impossible to locate the initial position of the acquisition card, which will increase the difficulty of one-to-one correspondence between the collected electrical signal and the capillary array
These two patents have a small number of channels, and parallel electrophoresis and signal acquisition of more channels are not possible

Method used

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  • A 128-channel array capillary electrophoresis instrument
  • A 128-channel array capillary electrophoresis instrument
  • A 128-channel array capillary electrophoresis instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A 128-channel array capillary electrophoresis instrument, including: double 45-degree angle mirrors 1, double 45-degree angle mirror support bases 2, absolute rotary encoders 4, absolute rotary encoder support bases 5, dual-axis miniature DC regulators Speed ​​motor 6, dual-axis miniature DC speed-regulating motor support base 7, polytetrafluoroethylene ring support plate 8, polytetrafluoroethylene ring 9, rotating mirror 10, waste liquid pool 11, laser-induced fluorescence collection device 12, laser-induced Fluorescence collection device support base 13, high-speed and high-precision data acquisition card with encoder read-in 14, laser source 15, photomultiplier tube 16, laser source support base 17;

[0022] The double 45-degree angle reflector 1 is installed on the support base 2 of the double 45-degree angle reflector, the laser source 15 is installed on the laser source support base 17,

[0023] The absolute rotary encoder 4 is installed on the support base 5 of t...

Embodiment 2

[0033] The repeatability test of the capillary signal intensity of each channel.

[0034] Such as figure 1 The invention is used for the performance test of a 128-channel array capillary electrophoresis instrument. A beam of excitation laser light is emitted from the laser source 15 and reflected to the laser-induced fluorescence collection device 12 through the double 45-degree angle mirrors 1 . The excitation laser is transmitted to the rotating mirror 10 through the laser-induced fluorescence collection device 12, and strikes on the capillary fixed on the polytetrafluoroethylene ring 9 to generate excitation laser-fluorescence signals. The excitation laser-fluorescence signal is transmitted to the photomultiplier tube 16 to amplify the optical signal, and then transmitted to the high-speed and high-precision data acquisition card 14 with an encoder for data acquisition.

[0035] The rotating mirror 10 is driven by a biaxial miniature DC speed regulating motor 6 to realize...

Embodiment 3

[0041] Interference test between signals of each channel.

[0042] Under the condition that the instrument is working normally, the capillary to be detected is not inserted, and the relationship between the signal intensity of the background noise of 128 channels is observed. Figure 5 It is a graph showing the relationship between the signal strength of the 128-channel background noise, and it can be seen that there is no interference from other signals.

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Abstract

A 128-channel array capillary electrophoresis instrument belongs to the technical field of electrophoresis. The technical points are: there is a PTFE ring support plate under the support base of the biaxial micro DC speed control motor, the PTFE ring is fixed on the PTFE ring support plate, and a rotating reflector is installed under the PTFE ring. Mirror, there is a waste liquid pool under the rotating mirror, the waste liquid pool is installed on the laser-induced fluorescence collection device, the laser-induced fluorescence collection device is installed on the support base of the laser-induced fluorescence collection device, and the high-speed and high-precision data read by the encoder The acquisition card is connected with the absolute rotary encoder. The beneficial effects are: the present invention can realize the one-to-one correspondence between the position information of 128 capillaries and the collected laser-induced fluorescence signals, thereby realizing the collection and recording of 128-channel sample signals; it can greatly improve work efficiency, improve detection accuracy, and reduce external Light interference, improve fluorescence collection efficiency, reduce manufacturing cost and use cost.

Description

technical field [0001] The invention belongs to the technical field of electrophoresis, in particular to a 128-channel array capillary electrophoresis instrument. Background technique [0002] With the development of science and technology, the collection, analysis and data processing of the same or similar samples with large amounts of data are becoming more and more important, such as the birth and development of new disciplines such as combinatorial chemistry, genomics, proteomics, and human genome projects. With the rapid development, there is a demanding and increasingly urgent demand for the rapid analysis capability of large quantities of similar types of micro-samples. Capillary electrophoresis, array capillary electrophoresis, chip capillary electrophoresis and other electrophoresis are developing rapidly. They can separate and detect inorganic ions, organic biomolecules, supramolecules and even cells. They are widely used in the field of analysis and have become an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N27/447
CPCG01N21/64G01N27/447
Inventor 王利牛海月林翔张枫
Owner DALIAN NATIONALITIES UNIVERSITY