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A flowering inducer and application of the flowering inducer and flowering process

A technology of inducer and process, applied in the directions of application, microorganism-based method, bacteria used in food preparation, etc., can solve the problems of inability to produce high-quality Fu tea, the effect of blooming is not obvious, etc., to improve the efficiency of blooming , Improve the effect of blooming, and speed up the effect of blooming

Active Publication Date: 2022-04-26
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no matter in the actual production or in the laboratory, we have found that the inoculation of S. coronoidis in black hair tea has no obvious flowering effect, and it is impossible to produce high-quality Fu tea.

Method used

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  • A flowering inducer and application of the flowering inducer and flowering process
  • A flowering inducer and application of the flowering inducer and flowering process
  • A flowering inducer and application of the flowering inducer and flowering process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 high-quality Fu tea and its processing technology

[0046] The process includes the following steps:

[0047]Preparation of tea raw materials: firstly take black hair tea and lightly pulverize it, then take a part of it and put it under 9K radiation metering for irradiation sterilization; take another part and put it in a high-pressure steam sterilizer at 121°C for 20 minutes, and then put it in an airless Under bacterial environment conditions, the tea raw materials obtained by the above two sterilization methods were respectively added to a 12-well cell culture plate in an amount of about 0.6 g per well, and set aside.

[0048] Preparation of S. coronis spore suspension: first fully activate S. coronis in a petri dish until it produces a large number of spores, then add sterile water on the surface of the culture medium, scrape the spores with a cotton swab to make coronoids Aqueous suspension of spores of Mycocystis spp. (600 CFU / mL, this concentration ...

Embodiment 2

[0078] Embodiment 2 high-quality hair flower white tea and its processing technology

[0079] The process includes the following steps:

[0080] Preparation of tea raw materials: First, take white tea and lightly crush it, then take a part of it and put it under 9K radiation metering for irradiation sterilization; take another part and put it in a high-pressure steam sterilizer at 121°C for 20 minutes, and then put it in an aseptic Under ambient conditions, the tea raw materials obtained by the above two sterilization methods were respectively added into a 12-well cell culture plate in an amount of about 0.6 g per well, and then used.

[0081] Preparation of the spore suspension of Cystis coronis: same as in Example 1.

[0082] Preparation of flower-inducing agent bacterial suspension: same as in Example 1.

[0083] Add the spore suspension of S. coronoidis prepared above to the above-mentioned 12-well cell culture plate with a ratio of volume to mass ratio (ml / g) of about 1...

Embodiment 3

[0092] Embodiment 3 high-quality hair flower oolong tea and its processing technology

[0093] The process includes the following steps:

[0094] Preparation of tea raw materials: First, take oolong tea and lightly crush it, then put it in a high-pressure steam sterilizer at 121°C for 20 minutes, and then add 12-well cells at an amount of about 0.6g per well under sterile conditions culture plate, ready to use.

[0095] Preparation of the spore suspension of Cystis coronis: same as in Example 1.

[0096] Preparation of flower-inducing agent bacterial suspension: same as in Example 1.

[0097] Add the spore suspension of S. coronoidis prepared above to the above-mentioned 12-well cell culture plate with a ratio of volume to mass ratio (ml / g) of about 1:30 (here can be adjusted accordingly according to the actual application in production) On the aseptic tea raw material, the single bacterial suspension or the mixed bacterial suspension was mixed with 10 6 CFU / mL or 10 5 CF...

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Abstract

The invention relates to a flowering inducer and the application and flowering process of the flowering inducer. The flowering inducer includes any one or a combination of strains of Bacillus, Lactobacillus, Microbacterium, Lactococcus and Bifidobacterium. The tea flowering process steps using the flowering inducer are: sterilizing the tea raw materials; adding S. coronis and the flowering inducer; The liquid is added to the tea raw material; the tea leaves bloom. The flowering inducer of the present invention can solve the problems of long flowering cycle, unstable flowering quality, and possible occurrence of harmful bacteria in the processing process of Fu tea and other flowering tea products. The invention can improve the flowering efficiency and enhance the flowering effect , and has a clear guarantee of microbial safety, it can provide high-quality probiotic Fu tea and other Fahua-type probiotic tea products containing one or several beneficial microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microorganisms applied to food processing, and in particular relates to a flowering inducer, the application of the flowering inducer and a flowering process. Background technique [0002] Eurotium cristatum, commonly known as "Golden Flower", is a fungus belonging to the genus Eurotium in the family Trichocomaceae of the order Eurotiales. Studies have shown that the bacterium has various health effects such as improving human immunity, regulating human metabolism, lowering fat, lowering blood pressure, and regulating blood sugar. Fu tea is a kind of dark tea unique to my country. Its endoplasmic fungus has a floral fragrance, pure and strong aroma, the soup color is orange, and the taste is mellow. One of the most important steps in its production process is a process commonly known as "fahua", that is, as the dominant bacterium, S. coronoidis grows vigorously in the raw material of Fucha - black hair ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23F3/14C12R1/07C12R1/225C12R1/01
CPCA23F3/14C12N1/20A23V2400/123A23V2400/137A23V2400/231A23V2400/513
Inventor 凌铁军刘路路李儒泽龙雁华张红张梁李大祥李立祥张正竹宛晓春
Owner ANHUI AGRICULTURAL UNIVERSITY
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