A compound used as antitumor drug and its production method
A compound and biological technology, applied in the direction of anti-tumor drugs, biochemical equipment and methods, drug combinations, etc., can solve the problems of difficult research and development of anti-tumor drugs, lack of high-efficiency, specific drugs, etc., and achieve a safe and reliable fermentation cycle with little pollution , the effect of simple steps
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Embodiment 1
[0042] 1. The pCold-TF-AOL_s00215g276 plasmid containing the target fragment of the farnesyltransferase gene
[0043] (1) The total RNA of Arthrobotrys oligospora 1.3170 (the strain was provided by the State Key Laboratory of Conservation and Utilization of Biological Resources in Yunnan) was extracted by column method. The process is as follows: culture Arthropodium oligospora 1.3170 in PDB medium at 37°C for 7 days, collect mycelium by filtration, add liquid nitrogen and grind until the fungus becomes powdery; then extract according to the instructions of the Tiangen Plant Genome Extraction Kit total RNA. Total RNA electrophoresis detection such as figure 1 shown.
[0044](2) Referring to the instructions of the Takara cDNA First Strand Synthesis Kit, use the Takara cDNA First Strand Synthesis Kit to reverse transcribe RNA into cDNA. The obtained cDNA was used as a template, and 276-F (as shown in SEQ ID No. 3) and 276-R (as shown in SEQ ID No. 4) were used as a pair of p...
Embodiment 2
[0075] The engineering strain BL21(DE3) / pCold-TF-AOL_s00215g276 obtained in the second subsection of Example 1 was fermented and subjected to subsequent compound purification. The cultivation and treatment steps of the strains are the same as those in Section 3 and Section 4 of Example 1, except that the 37°C in step (2) of Section 3 is changed to 35°C. Under these combined conditions, the three compounds II, III and IV in this application could be isolated.
Embodiment 3
[0077] The engineering strain BL21(DE3) / pCold-TF-AOL_s00215g276 obtained in the second subsection of Example 1 was fermented and subjected to subsequent compound purification. The cultivation and processing steps of the strains are the same as those in the third and fourth subsections of Example 1, except that the 37°C in the step (2) of the third subsection is changed to 39°C. Under these combined conditions, the three compounds II, III and IV in this application could be isolated.
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