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Microfluidic paper chip for chemiluminescence time resolution and detection as well as preparation method and application thereof

A microfluidic paper chip, chemiluminescence technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, etc., can solve the problems of limited use range, solution migration, troublesome operation, etc., to overcome the problem of sample solution and the effect of the influence of the chemiluminescence reaction signal

Active Publication Date: 2018-10-16
合肥庐阳科技创新集团有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the former has designed a straight-line flow channel, which requires a relatively long channel and a long length difference between channels to achieve better time-resolved detection of chemiluminescence. If the channel is too long, it is easy for the paper chip to exceed the instrument detection due to its large area. The area of ​​the light window, and the situation that it is easy for the solution to migrate to a certain length and cannot advance; the latter uses polydimethylsiloxane to modify the flow channel, which brings certain troubles to the operation. In addition, the polydimethylsiloxane in the channel Oxane, which may have some influence on the sample solution and chemiluminescent signal, thus limiting its scope of use

Method used

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  • Microfluidic paper chip for chemiluminescence time resolution and detection as well as preparation method and application thereof
  • Microfluidic paper chip for chemiluminescence time resolution and detection as well as preparation method and application thereof
  • Microfluidic paper chip for chemiluminescence time resolution and detection as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The specific production, assembly and testing process is as follows:

[0041] 1) First use Computer Aided Design (CAD) drawing software to design the pattern of the chemiluminescent time-resolved detection microfluidic paper chip (see attached figure 1 shown). The design parameters of the paper chip are as follows:

[0042] The circular sampling hole is connected to the first-level flow channel, part of the first-level flow channel is branched out from the second-level flow channel, and the second-level flow channel is deflected 60° in one direction to branch out into the third-level flow channel, and the third-level flow channel is deflected in the same direction 60° bifurcates into four-stage circulation channels. One end of each secondary circulation channel is connected to the middle part of the upper-level circulation channel, and the other end is connected to a circular detection hole.

[0043] The diameter of the above-mentioned circular sampling hole is 8 mm,...

Embodiment 2

[0049] In order to prove that the microfluidic paper chip of the present invention can realize the time-resolved detection of chemiluminescent signals, the inventors assembled a chemiluminescent functionalized microfluidic paper chip and used a chemiluminescent instrument to detect hydrogen peroxide. The specific method is as follows:

[0050] 1) First, use Photoshop CS4 software to design the pattern of the microfluidic paper chip for chemiluminescence time-resolved detection. The design parameters of the paper chip are as follows:

[0051] The circular sampling hole is connected to the first-level flow channel, part of the first-level flow channel branches out to the second-level flow channel, and the second-level flow channel is deflected by 60° in one direction to branch out to the third-level flow channel. One end of each secondary circulation channel is connected to the middle part of the upper-level circulation channel, and the other end is connected to a circular dete...

Embodiment 3

[0057] In order to prove the beneficial effect of the present invention, the inventor used the paper chip of Example 2 to prepare a chemiluminescent microfluidic paper chip for the simultaneous detection of glucose, uric acid and cholesterol. The specific method is as follows:

[0058] 1) First, prepare a microfluidic paper chip as described in step 1) of Example 2. In this embodiment, the total number of circulation channels of the microfluidic paper chip is three, and the number of detection holes is three.

[0059] 2) Chitosan was dissolved in 0.25% (v / v) glacial acetic acid, and a chitosan solution of 1 mg / mL was prepared, and 3.0 μL of the above-mentioned 1 mg / mL chitosan solution was added dropwise to the paper chip. In the three detection wells, dry at room temperature for 3 minutes.

[0060] 3) Add 3.0μL 50U / mL glucose oxidase and 0.15mg / mL horseradish peroxidase mixture to the glucose detection area labeled 1 on the paper chip; add 3.0μL 50U / mL urate oxidase and 0.15...

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Abstract

The invention discloses a microfluidic paper chip for chemiluminescence time resolution and detection as well as a preparation method and application thereof. The paper chip takes filter paper as a substrate; the substrate is provided with a sample feeding hole, a circulation channel and a detection hole; the circulation channel comprises a main circulation channel and a plurality of secondary circulation channels; one end of the main circulation channel is connected with the sample feeding hole and the other end of the main circulation channel is connected with the detection hole; deflectionflow splitting is carried out in the middle of the main circulation channel at a certain angle to form a second-grade circulation channel; one end of the second-grade circulation channel is connectedwith the middle of the main circulation channel and the other end of the second-grade circulation channel is connected with the detection hole, and so on; chitosan, specific oxidase and a chemiluminescence catalyst are sequentially filled into the detection hole. According to the microfluidic paper chip disclosed by the invention, a microfluidic flow path is designed so that time resolution and detection of chemiluminescence is realized; the microfluidic paper chip does not need to be modified, has a small area and can be used for simultaneously detecting glucose, uric acid, cholesterol and the like.

Description

technical field [0001] The invention relates to a chemiluminescent time-resolved detection microfluidic paper chip, a preparation method and application thereof, and belongs to the technical field of microfluidic paper chip production. Background technique [0002] Microfluidic paper chip is a paper-based microfluidic analysis technology platform developed in recent years. It uses paper as the production material and uses certain technical means, including wax printing, plasma treatment, inkjet printing, drawing and Laser lithography and other technologies process a hydrophilic and hydrophobic micro channel network with a certain structure on the paper. The microfluidic paper chip integrates a series of operations such as sampling, reaction, and detection, and has the advantages of low cost, portability, easy operation, less reagent consumption, fast analysis speed, good biocompatibility, and multiple detection. , environmental monitoring, food safety analysis, rapid detect...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 李芳刘家昌张凯琦汪吉杭何建波
Owner 合肥庐阳科技创新集团有限公司
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