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Immobilized yeast continuous fermentation technology

An immobilized yeast, continuous technology, applied in fermentation, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc., can solve problems such as poor results, achieve low cost, simple filling, The effect of easy removal and maintenance

Inactive Publication Date: 2018-10-19
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a continuous fermentation technology of immobilized yeast to solve the problems of poor effect in the prior art

Method used

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  • Immobilized yeast continuous fermentation technology
  • Immobilized yeast continuous fermentation technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Using synthetic medium as fermentation raw material and adopting bamboo fiber immobilized yeast to continuously ferment fuel ethanol

[0046] First, fix cotton fibers (thickness 0.2 cm) cut to a side length of 8 cm on polyvinyl chloride such as figure 1 On the central axis of a spherical grid with a diameter of 8 cm as shown (the side length of the ball hole is 1 cm), an immobilization medium is prepared; then the immobilization medium is 2 The filling amount of / L is placed in a 20L reactor with a height-to-diameter ratio of 2:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 15 L / h for 42 hours. Until the bacterial cell adsorption concentration is 200 million / ml. Then drain to waste. Then feed the fermentation medium (glucose concentration is 200g / L), the liquid content is 72%, and carry out circular fermentation, wherein the temperature is 34°C, the pH of the fermentation broth is 4.2, and the flow rate is 15L / h. The ...

Embodiment 2

[0047] Example 2 Using cassava liquefied mash medium as fermentation raw material and adopting bamboo fiber immobilized yeast to continuously ferment fuel ethanol

[0048] First, fix the cylindrical fiber (thickness 0.2cm) cut into a side length of 4cm on a polytetrafluoroethylene such as figure 1 On the central axis of a spherical grid with a diameter of 3 cm as shown (the side length of the ball hole is 1 cm), an immobilization medium is prepared; then the immobilization medium is 90 cm 2The filling amount per L is placed in a 10L reactor with an aspect ratio of 2.5:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 10 L / h for 48 hours. Until the cell adsorption concentration is 220 million cells / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2.5, add liquefaction enzyme (15U / g cassava dry weight) to liquefy at 85°C for 2 hours, press filter, and discharge the waste liquid of immobilized seeds. Then add cassav...

Embodiment 3

[0050] Example 3 Using molasses culture medium as fermentation raw material and adopting activated carbon fiber to immobilize yeast for continuous fermentation to produce fuel ethanol

[0051] First, the activated carbon fiber cut into a diameter of 5 cm was fixed in a spherical grid with a diameter of 4 cm made of polytetrafluoroethylene (the side length of the mesh was 0.5 cm) to prepare an immobilization medium; 2 The filling amount per L is placed in a 20L reactor with an aspect ratio of 1.8:1. Then the cultured strains were added to the fermenter, and fixed at a flow rate of 30 L / h for 32 hours. Until the bacterial cell adsorption concentration is 200 million / ml. Then the acidified molasses was diluted to a sugar concentration of 250g / L, and the immobilized seed waste liquid was discharged. Then add molasses, the liquid content is 75%, and carry out circular fermentation, wherein the temperature is 35 ° C, the pH of the fermentation liquid is 4.4, and the flow rate is 3...

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Abstract

The invention discloses an immobilized yeast continuous fermentation technology. The technology comprises the following steps: a fiber material is fixed in a spherical grid as an immobilization medium, and at the same time, the immobilized medium is filled in 1-5 fermentation cylinders in series connection, and the yeast is fixed on a fiber carrier by adsorption effects and fermented. The immobilized yeast is stably and continuously fermented by switching the fermentation cylinder sequence, in order to maintain activity of the immobilized yeast in the terminal fermentation cylinders. The immobilization method has the advantages of low input cost, convenient operation, stable process, good adsorption effect, and long-lasting and high activity of the immobilized yeast.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to an immobilized yeast continuous fermentation technology. Background technique [0002] Due to the overexploitation of fossil energy and other non-renewable energy sources, the energy crisis has become the primary problem to be solved in the development of the world today. Finding new alternative energy sources has become the focus of energy strategies over the years. Ethanol is not only a renewable fuel, but also can be mixed with gasoline in any proportion to ease the pressure on oil demand, and it has always been a good energy substitute. At present, ethanol production mainly relies on biological fermentation. Due to the wide range of raw material sources (such as corn, cassava, straw, etc.), it has great development potential. [0003] Traditional ethanol fermentation adopts a large inoculum continuous fed-batch (fed-feeding) method for free fermentati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C12N11/08C12N11/14C12P7/10
CPCC12N11/02C12N11/08C12N11/14C12P7/10Y02E50/10
Inventor 应汉杰刘庆国陈勇邹亚男赵南刘桂文
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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