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Garlic antitumor active polypeptide

A technology with anti-tumor activity and garlic, which is applied in the field of garlic anti-tumor active peptides, can solve the problems of insufficient purity and low yield of garlic peptides, and achieve the effects of improving purity and quality, improving antioxidant capacity, and increasing yield and activity

Inactive Publication Date: 2018-10-19
金华市景和科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the yield of the garlic polypeptide is low and the purity is not enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing a garlic anti-tumor active polypeptide. The garlic powder is extracted with alkali to obtain a garlic protein extract, and then protease is added to the garlic protein extract to obtain an enzymolysis solution. The enzymolysis solution is subjected to ultrafiltration, gel column chromatography and High-performance liquid chromatography treatment, that is, the active substance of garlic polypeptide. The preparation method uses garlic powder to prepare garlic active polypeptides with great pharmaceutical value, which has very important production significance and economic value. The preparation method is simple and feasible, the process is stable, and the reproducibility is high. The equipment used is mature equipment. , low investment cost, high recovery rate of peptide active substances.

[0022] A preparation method of garlic anti-tumor active polypeptide, the specific steps are:

[0023]1) Alkali extraction: Add garlic powder to NaOH solution w...

Embodiment 2

[0032] A preparation method of garlic anti-tumor active polypeptide, the specific steps are:

[0033] 1) Alkali extraction: add garlic powder to NaOH solution with a concentration of 1.5% according to the ratio of solid to liquid 1:33 (g / mL), extract with alkali for 1.5 hours, then centrifuge, and the supernatant is the garlic protein extract;

[0034] 2) Protease enzymatic hydrolysis: Preheat the garlic protein extract to 35°C, adjust its pH to 8.0 with phosphate buffer, add papain and elastase for constant temperature enzymolysis for 4 hours, kill the enzyme after hydrolysis, and dissolve the hydrolyzate Leave to cool, centrifuge, and the supernatant is the enzymatic hydrolysis solution;

[0035] 3) Ultrafiltration: Separate the enzymatic hydrolysis solution with 8kDa, 4kDa, and 0.6kDa ultrafiltration membranes in sequence. The ultrafiltration pressure is 0.05MPa, and the ultrafiltration time is 20min. The components after ultrafiltration are collected to obtain garlic activ...

Embodiment 3

[0041] A preparation method of garlic anti-tumor active polypeptide, the specific steps are:

[0042] 1) Alkali extraction: add garlic powder to NaOH solution with a concentration of 1.5% according to the ratio of solid to liquid 1:33 (g / mL), extract with alkali for 1.5 hours, then centrifuge, and the supernatant is the garlic protein extract;

[0043] 2) Protease enzymatic hydrolysis: Preheat the garlic protein extract to 35°C, adjust its pH to 8.0 with phosphate buffer, add papain and elastase for constant temperature enzymolysis for 4 hours, kill the enzyme after hydrolysis, and dissolve the hydrolyzate Leave to cool, centrifuge, and the supernatant is the enzymatic hydrolysis solution;

[0044] 3) Ultrafiltration: Separate the enzymatic hydrolysis solution with 8kDa, 4kDa, and 0.6kDa ultrafiltration membranes in sequence. The ultrafiltration pressure is 0.05MPa, and the ultrafiltration time is 20min. The components after ultrafiltration are collected to obtain garlic activ...

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Abstract

The invention discloses a garlic antitumor active polypeptide. A preparation method of the garlic antitumor active polypeptide comprises the following steps: carrying out alkali extraction on garlic powder to obtain garlic protein extract, adding protease to the garlic protein extract, carrying out enzymatic hydrolysis to obtain an enzymatic hydrolysate, and carrying out ultrafiltration, gel column chromatography and high performance liquid chromatography on the enzymatic hydrolysate to obtain the garlic polypeptide active substance. The active polypeptide has the advantage of high purity, high quality, high activity and good antitumor effect, and the preparation method of the active polypeptide has the advantages of simplicity, feasibility, low cost, stable process, removal of a large amount of salts, and high yield.

Description

technical field [0001] The invention relates to the technical field of bioactive component extraction, in particular to a garlic anti-tumor active polypeptide. Background technique [0002] Garlic is a perennial herbaceous root plant of the Liliaceae Allium genus, commonly known as gourd, gourd garlic, single garlic, etc. Garlic has been planted and eaten in various parts of my country and continues to this day, becoming a favorite and indispensable food for people. The unique pungent taste of garlic has become an indispensable seasoning on people's tables. At the same time, people have gradually discovered the medicinal value of garlic since ancient times. At home and abroad, there are a large number of data and literature to verify the pharmacological effects of garlic. Its effects are: antibacterial and bacteriostatic effects; Function; anti-tumor effect, among which the most attracting people's attention are the functions of disease prevention, treatment, sterilization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
CPCC07K1/16C07K1/34C07K1/36C12P21/06
Inventor 汪珂
Owner 金华市景和科技有限公司
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