Method for making compost on basis of household waste

A domestic garbage and composting technology, which is applied in the field of solid waste recycling, achieves the effects of low surface tension, simple preparation method, and low cost

Inactive Publication Date: 2018-10-19
中林山水(北京)生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A single biosurfactant has certain limitations in improving composting efficiency

Method used

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  • Method for making compost on basis of household waste
  • Method for making compost on basis of household waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The seed liquid preparation of embodiment 1. bacterial classification

[0034] 1. Slope activation

[0035] Bacillus subtilis and Candida lipolytica were respectively inoculated in fresh slant medium, and cultured in a constant temperature incubator at 30°C for 48 hours.

[0036] The composition of the slant medium is: beef extract 2.0g / L; peptone 5.0g / L; NaCl 5.0g / L; agar 20.0g / L; pH 7.0. The prepared slant medium was sterilized by high-pressure steam at 121°C for 20 minutes, cooled to room temperature before use.

[0037] 2. Seed liquid culture

[0038] Preparation of seed liquid medium: Weigh 200.0g of peeled potatoes, cut them into 1 cm square pieces, add 1000mL of water, boil and keep boiling for 30 minutes on low heat, filter with three layers of gauze, make up the filtrate to 1000mL, add 20.0g of sucrose , natural pH. Autoclaved at 121°C for 20 minutes, cooled to room temperature before use.

[0039]Inoculation: under aseptic operation conditions, use asepti...

Embodiment 2

[0042] Embodiment 2. Preparation of composite biosurfactant

[0043] The seed solution of bacillus subtilis and candida lipolytica is mixed according to the number ratio of live bacteria of 4-7:3-8 to obtain a composite microbial agent. The composite microbial agent is inoculated in the medium for fermentation according to the volume percentage of 4%-6%, cultured for 46-50 hours, and the obtained fermentation liquid is the composite biosurfactant.

[0044] The culture conditions are: temperature 32-34°C, stirring speed 440-570r / min, ventilation volume 1.5-1.8vvm.

[0045] The formula of the medium for fermentation is: Na 2 HPO 4 4~4.5g / L,KH 2 PO 4 3.5~4.5g / L, NH 4 NO 3 2.5~3.0g / L, MgSO 4 0.09-0.10g / L, CaCl 2 2H 2 O 0.02-0.03g / L, NaCl 0.05-0.10g / L, trace element solution 0.8-1.0mL / L, glucose 35-45g / L, pH 6.5-7.0. Among them, the formula of the trace element solution is: ZnSO 4 2.3-4mg / L, MnCl 2 4H 2 O 178-200mg / L, CoCl 2 ·6H 2 O 5-12mg / L, CuSO 4 ·5H 2 O 3-...

experiment example 1

[0049] Bacteria for fermentation: mix the seed liquids of Bacillus subtilis and Candida lipolytica according to the number ratio of live bacteria of 4:8 to obtain a composite bacterial agent.

[0050] Inoculate the composite bacterial agent in the fermentation medium at a volume percentage of 4%, control the temperature at 34°C, stir at 440r / min, and ventilate at 1.5vvm, and ferment for 50 hours to obtain a fermentation broth.

[0051] The formula of the medium for fermentation is: Na 2 HPO 4 4.3g / L, KH 2 PO 4 4g / L, NH 4 NO 3 2.7g / L, MgSO 4 0.10g / L, CaCl 2 2H 2 O 0.03g / L, NaCl 0.08g / L, trace element solution 1.0mL / L, glucose 40g / L, pH 6.8, solvent is distilled water.

[0052] The formula of the trace element solution is: ZnSO 4 3mg / L, MnCl 2 4H 2 O 190mg / L, CoCl 2 ·6H 2 O 9mg / L, CuSO 4 ·5H 2 O 7mg / L, FeC 6 h 5 o 7 ·5H 2 O 3g / L, the solvent is distilled water.

[0053] After testing, the surface tension value of the fermentation broth was 24.1mN / m.

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Abstract

The invention belongs to the field of solid waste recycling, and particularly relates to a method for making compost on the basis of household waste. The method is characterized by comprising the following step of preparing a composite biological surface activating agent which is prepared from composite fungicide containing Bacillus subtilis and Candida lipolytica through fermentation culture. Thesurface tension of the composite biological surface activating agent is lower than 25mN / m. After the composite biological surface activating agent is mixed with the household waste, the compost efficiency can be obviously improved.

Description

technical field [0001] The invention belongs to the field of solid waste recycling, and in particular relates to a method for producing compost based on domestic waste. Background technique [0002] Municipal solid waste treatment is a universal problem faced by human beings, which is directly related to the coordinated development of population, resources and environment. At present, composting, as an effective measure for the recycling of waste resources, has been widely used in many countries and regions in the world. The composting process is to use microorganisms (including bacteria, yeast, actinomycetes, fungi, and protozoa, etc.) to biochemically degrade organic matter in solid waste under certain temperature, humidity, and pH conditions, forming a humus-like soil. The factors affecting decomposing mainly include organic matter content, moisture, ventilation, temperature, carbon-nitrogen ratio, pH value, etc. With the increase of urban population, the efficiency of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C05F9/04C12R1/125C12R1/73
CPCC05F9/04C12P39/00Y02A40/20
Inventor 李楠任瑞霞
Owner 中林山水(北京)生态科技有限公司
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