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Kit and system for predicting susceptibility of female breast malignant tumors

A malignant tumor and susceptibility technology, applied in the field of biomedicine, can solve the problems of impossibility, inability to early warning of tumor susceptibility, unscientific and other problems

Active Publication Date: 2018-10-19
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the occurrence and development of tumors is a very complicated process, and it is obviously impossible and unscientific to use the changes of a single molecular genetic marker to diagnose the disease
With the existing technical means, it is not possible to provide a more accurate early warning of tumor susceptibility only through genetic information, and the current early identification and prediction methods for tumors need to be improved

Method used

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  • Kit and system for predicting susceptibility of female breast malignant tumors
  • Kit and system for predicting susceptibility of female breast malignant tumors
  • Kit and system for predicting susceptibility of female breast malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1 A Prediction System for Susceptibility to Female Breast Malignant Tumors

[0109] A female breast cancer susceptibility prediction system, comprising:

[0110] A device for obtaining the number of repetitions of the STR site short tandem sequence of the sample DNA;

[0111] Data processing and judging device, it comprises following module (as figure 1 ):

[0112] The data input module is used to input the age, gender, and repeat times of the short tandem sequence of the subject;

[0113] The database management module is used for the operation management of data storage, modification, deletion, query and printing;

[0114] The data calculation module is used to calculate the result of the discriminant function according to the number of repetitions of the STR site short tandem sequence in the data input module;

[0115] The analysis, discrimination and result output module is used to compare the results of the discriminant function, so as to predict the s...

Embodiment 2

[0150] Example 2 A kit for predicting susceptibility to female breast cancer

[0151] A female breast cancer susceptibility prediction kit, which includes the following components: STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, STR-6 primers, PCR amplification Increased reaction solution, LIZ-500 molecular weight internal standard, deionized formamide, instruction manual.

[0152] The concentrations of the above STR-1 primers, STR-2 primers, STR-3 primers, STR-4 primers, STR-5 primers, and STR-6 primers are all 10 μM, and the primer sequences are shown in the following table:

[0153]

[0154]

[0155] The PCR amplification reaction solution is a mixture of the following reagents: TaqDNA polymerase (5U / μL), Tris-HCl (100mM, pH 8.8 at 25°C), KCl (500mM), ethylphenyl polyethylene glycol (0.8 %(v / v)), MgCl 2 (25mM), dNTP (10mM), deionized water.

[0156] The PCR amplification reaction solution is stored at -20°C; the LIZ-500 molecular weight i...

Embodiment 3

[0158] Example 3 Using the system of Example 1 and the kit of Example 2 to predict the risk of the subject suffering from female breast cancer

[0159] Subject: Female, 52 years old, was admitted to the Breast Surgery Department of the Second Hospital of Jilin University. After being fully informed of the purpose and use of the examination, and on the premise of her willingness, she signed an informed consent and collected 1 mL of anticoagulant blood through a peripheral vein.

[0160] Use the kit of embodiment 2, carry out the following steps according to the method recorded on the kit instruction manual:

[0161] (1) Extract sample DNA: Use a DNA extraction kit to extract blood genomic DNA;

[0162] (2) PCR reaction

[0163] (2-1) Take out STR-1 primer, STR-2 primer, STR-3 primer, STR-4 primer, STR-5 primer, STR-6 primer, PCR amplification reaction solution from the refrigerator, equilibrate to room temperature, each After the components are fully dissolved, quickly centri...

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Abstract

The invention relates to a kit for predicting susceptibility of female breast malignant tumors. The kit is prepared from the following components of a primer STR-1, a primer STR-2, a primer STR-3, a primer STR-4, a primer STR-5, a primer STR-6, a PCR (polymerase chain reaction) amplified reaction liquid, a LIZ-500 molecular weight internal marker, and deionized formamide. The kit for predicting the susceptibility of the female breast malignant tumors can be used for the diagnosis of the female breast malignant tumors and the prediction of the sensitivity. The invention also provides a system for predicting the susceptibility of the female breast malignant tumors.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, it relates to a female breast cancer susceptibility prediction kit and a female breast cancer susceptibility prediction system. More specifically, the present invention relates to a kit for detecting STRs of genes related to susceptibility to female breast cancer susceptibility through short tandem repeats (Shorttandem repeats, STR for short) locus fragment analysis method, and by combining discriminant analysis statistical methods, to This provides an early warning of the subject's susceptibility to female breast cancer. Background technique [0002] Tumor is a disease closely related to genetics. To study the molecular genetic basis of tumors, and then propose tumor-specific genetic markers, it is hoped that it can be used for routine detection, clinical diagnosis, personalized treatment, and disease tracking after recovery. Etc. provides a simple and feasible method. However...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12M1/34
CPCC12Q1/6886C12Q2600/156
Inventor 郝书弘王晓峰杨麒巍任明
Owner JILIN UNIV
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