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Mobile phase formula for liquid chromatography detection of carotenoids in fruits and hplc method for detection of carotenoids in fruits

A carotene and liquid chromatography technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to achieve separation effect, different effects of mobile phase separation, etc., and achieves fast peak time, strong versatility, good separation effect

Active Publication Date: 2020-12-15
ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mobile phases reported in most studies are only suitable for specific detection samples and chromatographic columns. Once the detection conditions are changed, the ideal separation effect is often not achieved; in addition, due to the different carotenoid content and components in different samples, the flow The effect of phase separation is also different

Method used

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  • Mobile phase formula for liquid chromatography detection of carotenoids in fruits and hplc method for detection of carotenoids in fruits
  • Mobile phase formula for liquid chromatography detection of carotenoids in fruits and hplc method for detection of carotenoids in fruits
  • Mobile phase formula for liquid chromatography detection of carotenoids in fruits and hplc method for detection of carotenoids in fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] An HPLC method for the detection of carotenoids in corn, comprising the following steps:

[0093] (A) Preparation of carotenoid test samples:

[0094] (a) Mix the pulverized corn samples (take three different varieties of corn samples, denoted as corn 1, corn 2 and corn 3 respectively) with a mixture of acetone and n-hexane with a volume ratio of 1:1, and filter them with suction. , until the extract is colorless;

[0095] (b) combine the extracts in step (a), concentrate under reduced pressure to dryness to obtain acetone crude extract;

[0096] (c) dissolving the dried acetone crude extract with methanol, then adding an equal volume of 30% methanol-KOH for saponification more than 4 hours to obtain a saponified solution;

[0097] (d) concentrating the saponified liquid under reduced pressure to a minimum volume, adding a mixture of ethyl acetate and n-hexane with a volume ratio of 1:1 for extraction until the extract is colorless;

[0098] (e) combining the extracts ...

Embodiment 2

[0110] The HPLC method for the detection of carotenoids in tomato has the same detection steps as in Example 1, except that the chromatographic column used is a Vydac 201TP54 chromatographic column.

[0111] Shown in Fig. 2 (a) and Fig. 2 (b) are respectively the HPLC collection of illustrative plates of carotenoids in yellow tomato and blue tomato, and wherein peak 1 is lutein, and peak 2 is β-carotene, as can be seen, in From 4 minutes to 12 minutes, the lutein and β-carotene in the yellow tomato can be separated, the peak shape is good, and β-carotene is not detected in the green tomato.

Embodiment 3

[0113] The HPLC method for the detection of carotenoids in watermelon, the detection instrument and the chromatographic conditions are the same as in Example 2, the difference is that the present embodiment adopts two different separation methods for the same watermelon material, one utilizes an isocratic mobile phase to carry out Separation, that is (A) 0min-20min, mobile phase A 25%, mobile phase B 75%; another separation using gradient mobile phase, that is (B) 0min-8min, mobile phase A 100%, mobile phase B 0 %; 8min-13min, mobile phase A decreased from 100% to 0%, mobile phase B increased from 0% to 100%: 13min-20min, mobile phase A 0%, mobile phase B 100%.

[0114] Shown in Figure 3 (a) and Figure 3 (b) is the HPLC collection of illustrative plates that utilize gradient mobile phase to separate carotenoids in watermelon, wherein peak 1 is lycopene, and peak 2 is β-carotene, as can be seen, in Lycopene and β-carotene can be separated within 6 minutes to 12 minutes, and the...

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Abstract

Relating to the chromatographic detection field, the invention specifically provides a mobile phase formula for detection of carotenoid in fruits by liquid chromatography and an HPLC (high performanceliquid chromatography) method for detection of carotenoid in fruits. The mobile phase formula includes a mobile phase A and a mobile phase B, the mobile phase A comprises the following components acetonitrile, methanol, dichloromethane and n-hexane in a volume ratio of (80-90):(1-8):(1-8):(1-8); and the mobile phase B includes the following components acetonitrile, methanol, dichloromethane and n-hexane in a volume ratio of (50-60):(5-15):(15-20):(15-20). The mobile phase formula has a good separation effect on carotenoid in fruits, can be used for determination of carotenoid content and components in various fruit samples, is suitable for most C18 chromatographic columns, and has the advantages of strong universality, sensitivity and good repeatability.

Description

technical field [0001] The invention relates to the field of chromatographic detection, in particular to a mobile phase formulation for detecting carotenoids in fruits by liquid chromatography and an HPLC method for detecting carotenoids in fruits. Background technique [0002] Carotenoids are the most widely distributed and most abundant natural pigments in nature, and are widely found in plants, animals and algae. Carotenoids are usually referred to as C 40 The general term for two types of pigments of hydrocarbons (carotene) and their oxidized derivatives (lutein), which are structurally condensed by 8 isoprene units, typically C 40 Carotenoids carry an ionone ring, and hydrogen atoms at different positions on the ring can be replaced by hydroxyl, carbonyl, and epoxy groups. In plants, carotenoids act as auxiliary pigments for chloroplast photosynthesis and protect chlorophyll from damage by strong light, and are also precursors for the synthesis of plant hormone ABA (a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/34G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N30/34G01N2030/062
Inventor 康保珊古勤生刘慧刘丽锋吴会杰彭斌
Owner ZHENGZHOU FRUIT RES INST CHINESE ACADEMY OF AGRI SCI