Application of Flos Hydrangeae Macrophyllae extract in preparation of anti-HIV drug and preparation method of Flos Hydrangeae Macrophyllae extract

A technology of hydrangea and its extract, which is applied to the application of hydrangea extract in the preparation of anti-HIV drugs and its preparation, which can solve the problems of less research and development of hydrangea, and achieve the purpose of expanding uses, expanding channels, and increasing added value Effect

Inactive Publication Date: 2018-10-23
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, foreign scientists have conducted more research and development on the active ingredients

Method used

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  • Application of Flos Hydrangeae Macrophyllae extract in preparation of anti-HIV drug and preparation method of Flos Hydrangeae Macrophyllae extract
  • Application of Flos Hydrangeae Macrophyllae extract in preparation of anti-HIV drug and preparation method of Flos Hydrangeae Macrophyllae extract
  • Application of Flos Hydrangeae Macrophyllae extract in preparation of anti-HIV drug and preparation method of Flos Hydrangeae Macrophyllae extract

Examples

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Example Embodiment

[0041] The embodiment of the present invention: a preparation method of anti-hydrangea extract, including the following process steps:

[0042] 1.1 Extraction of hydrangea extract: Weigh 500g of dried hydrangea leaves, pulverize, use a 1:5 ratio of material to liquid, and reflux the ethanol solution with a volume fraction of 60% to 95% for 3 times, respectively 4h, 3h, 2h, The extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract without alcohol. Add appropriate amount of distilled water to the extract, ultrasonically dissolve, and filter to obtain the extract aqueous solution.

[0043] 1.2 Extraction of different polar parts of the extract aqueous solution: extract the raw extract aqueous solution with ethyl acetate for 4 times, and then extract the extract aqueous solution with n-butanol 4 times to obtain the n-butanol After the extracts are combined, they are concentrated under reduced pressure to obtain the n-butanol layer extract. The ...

Example Embodiment

[0045] Example 2 of the present invention: the application of hydrangea extract in the preparation of anti-HIV drugs, Fr.1 to Fr.10 obtained in the preparation of the examples were prepared by adding various pharmaceutically acceptable excipients according to the dosage requirements Various dosage forms of Chinese medicine preparations.

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Abstract

The invention discloses application of a Flos Hydrangeae Macrophyllae extract in preparation of anti-HIV drug and a preparation method of the Flos Hydrangeae Macrophyllae extract. The preparation method comprises the steps of taking Flos Hydrangeae Macrophyllae dry leaves as raw materials, carrying out refluxing extraction by using an ethanol solution with the volume fraction being 60%-95%, filtering, concentrating under reduced pressure to obtain extractum, adding a proper amount of distilled water, ultrasonically dissolving, and filtering to obtain an extractum aqueous solution; extracting the extractum aqueous solution twice to four times with ethyl acetate and n-butanol which have the same volume successively, concentrating under reduced pressure to obtain a n-butanol layer extractum,separating and purifying the n-butanol layer extractum by using a positive phase silica gel column chromatography, carrying out gradient elute with chloroform-methanol, tracking, detecting and mergingthe same fractions according to TLC, and carrying out anti-HIV activity detection to obtain a chloroform-methanol fraction with the corresponding volume ratio. The chloroform-methanol fraction is ananti-HIV effective ingredient, the down-regulating and controlling rate of the effective ingredient to an HIV-1 LTR sequence is 93.5%, an anti-HIV virus raw material channel is expanded, the application of Flos Hydrangeae Macrophyllae is expanded, and the additional value of the Flos Hydrangeae Macrophyllae can be increased remarkably.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to the application of hydrangea flower extract in the preparation of anti-HIV medicine and its preparation method. Background technique [0002] AIDS (Acquired immunodeficiency syndrome, AIDS) is an infectious disease caused by the infection of human immunodeficiency virus (HIV), which damages CD4+ T lymphocytes and other immune cells, resulting in the complete loss of collective immune function. Since AIDS was discovered in the world in 1981, AIDS has become one of the three major killers that endanger human life and health today. At present, the drugs used clinically to prevent and treat AIDS are mainly chemical synthetic drugs, which will produce strong toxic side effects and drug resistance for the treatment of AIDS. So far, many natural compounds have been found to have anti-HIV activity, and they have the advantages of specificity, less side effects, conv...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P31/18A61K127/00
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55A61P31/18
Inventor 田民义周英洪怡刘雄利林冰彭礼军王慧娟
Owner GUIZHOU UNIV
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