PCA-SVM-based apostichopus japonicus origin identification method
A technology of imitation sea cucumber and origin, applied in the identification of the origin of imitation sea cucumber, and the field of identification of the origin of imitation sea cucumber, can solve the problems of few production areas, inability to consumers, and no establishment of a production area identification model.
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Embodiment 1
[0030] Embodiment 1 (fatty acid relative content model):
[0031] A. Collection of imitation japonicus samples from different origins
[0032] Collected 54 samples of the relative content of fatty acids in imitation japonicus, including 6 samples from Changhai County; 6 samples from Zhangzidao; 6 samples from Xiapu, Fujian; 6 samples from Pulandian Pikou; 6 samples from Wafangdian; 6 samples; 6 samples from Danzi Island, Yantai; 6 samples from Laizhou, Yantai; The samples from Rushan, Weihai were cultured in captivity; the samples from Xiapu, Fujian were cultured in rafts.
[0033] Except for the Fujian Xiapu sample, which is the adult ginseng of the same year, the other samples are all 2-year-old adult ginseng, with a body length of 15-19 cm and a weight of 100-130 g. Immediately after catching, the samples were washed with water to remove viscera, sand and lime rings, and the body wall was washed with ultrapure water, freeze-dried for 48 hours, ground into powder, passed t...
Embodiment 2
[0055] Embodiment 2 (fatty acid carbon stable isotope composition model):
[0056] A. Collection of imitation japonicus samples from different origins
[0057] Collected 55 samples of the carbon stable isotope composition of japonicus japonicus fatty acids, including 6 samples from Changhai County; 6 samples from Zhangzidao; 6 samples from Xiapu, Fujian; 7 samples from Pulandian Pikou; 6 samples from Wafangdian; 6 samples from Rushan, Weihai; 6 samples from Danzi Island, Yantai; 6 samples from Laizhou, Yantai; 6 samples from Muping, Yantai, of which the samples from Changhai County, Zhangzi Island and Yantai were bottom-seeding; The samples in Diandian and Rushan, Weihai were cultured in captivity; the samples in Xiapu, Fujian were cultured in rafts.
[0058] Except for the Fujian Xiapu sample, which is the adult ginseng of the same year, the other samples are all 2-year-old adult ginseng, with a body length of 15-19 cm and a weight of 100-130 g. Immediately after catching, ...
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