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PCA-SVM-based apostichopus japonicus origin identification method

A technology of imitation sea cucumber and origin, applied in the identification of the origin of imitation sea cucumber, and the field of identification of the origin of imitation sea cucumber, can solve the problems of few production areas, inability to consumers, and no establishment of a production area identification model.

Inactive Publication Date: 2018-10-30
DALIAN MARITIME UNIVERSITY
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Problems solved by technology

[0004] Liu Xiaofang et al. used inductively coupled plasma mass spectrometry (ICP-MS) to measure AL, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Se, The contents of 15 inorganic elements including Mo, Cd, Hg and Pb were preliminarily distinguished by cluster analysis and principal component analysis, but the number of samples was too small and the differentiation effect of some production areas was not good. Obvious problems, and no specific origin identification model has been established, so it cannot be actually used by consumers
[0005] Zou Xiaobo et al. used near-infrared spectroscopy to measure the collagen content of 43 imitation japonicus samples from 4 regions in Dalian, Fujian, Lianyungang, and Shandong by spectrophotometer method, and established the imitation japonicus samples from different origins by partial least squares method. A sea cucumber identification model, but there are too few origins selected for samples, and the origin identification model has not been further optimized for parameters.

Method used

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  • PCA-SVM-based apostichopus japonicus origin identification method

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Embodiment 1

[0030] Embodiment 1 (fatty acid relative content model):

[0031] A. Collection of imitation japonicus samples from different origins

[0032] Collected 54 samples of the relative content of fatty acids in imitation japonicus, including 6 samples from Changhai County; 6 samples from Zhangzidao; 6 samples from Xiapu, Fujian; 6 samples from Pulandian Pikou; 6 samples from Wafangdian; 6 samples; 6 samples from Danzi Island, Yantai; 6 samples from Laizhou, Yantai; The samples from Rushan, Weihai were cultured in captivity; the samples from Xiapu, Fujian were cultured in rafts.

[0033] Except for the Fujian Xiapu sample, which is the adult ginseng of the same year, the other samples are all 2-year-old adult ginseng, with a body length of 15-19 cm and a weight of 100-130 g. Immediately after catching, the samples were washed with water to remove viscera, sand and lime rings, and the body wall was washed with ultrapure water, freeze-dried for 48 hours, ground into powder, passed t...

Embodiment 2

[0055] Embodiment 2 (fatty acid carbon stable isotope composition model):

[0056] A. Collection of imitation japonicus samples from different origins

[0057] Collected 55 samples of the carbon stable isotope composition of japonicus japonicus fatty acids, including 6 samples from Changhai County; 6 samples from Zhangzidao; 6 samples from Xiapu, Fujian; 7 samples from Pulandian Pikou; 6 samples from Wafangdian; 6 samples from Rushan, Weihai; 6 samples from Danzi Island, Yantai; 6 samples from Laizhou, Yantai; 6 samples from Muping, Yantai, of which the samples from Changhai County, Zhangzi Island and Yantai were bottom-seeding; The samples in Diandian and Rushan, Weihai were cultured in captivity; the samples in Xiapu, Fujian were cultured in rafts.

[0058] Except for the Fujian Xiapu sample, which is the adult ginseng of the same year, the other samples are all 2-year-old adult ginseng, with a body length of 15-19 cm and a weight of 100-130 g. Immediately after catching, ...

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Abstract

The invention discloses a PCA-SVM-based apostichopus japonicus origin identification method. The method comprises the steps of collecting apostichopus japonicus samples of different origins; measuringfatty acid relative content of the apostichopus japonicus samples of different origins, or measuring fatty acid stable carbon isotope composition of the apostichopus japonicus samples of different origins; and building a PCA-SVM joint model by combining principle component analysis (PCA), a support vector machine (SVM) and a particle swarm optimization (PSO) algorithm. According to the method, fatty acid with a food source characteristic is taken as a geographical origin mark, data of the fatty acid relative content and the fatty acid stable carbon isotope composition of the apostichopus japonicus of different origins is lowered in dimension through PCA so as to be used as input of the model, after samples are extracted, an SVM classifier is optimized constantly through cross validation and the PSO algorithm, so as to form the joint model, the apostichopus japonicus origin can be identified objectively and accurately, and the final origin identification rates reach 79.49% and 98.33%.

Description

technical field [0001] The invention belongs to the technical field of authenticity identification of geographical indication products, and relates to a PCA-SVM-based identification method for the origin of sea cucumber imitation, that is, using gas chromatography and stable isotope mass spectrometry to measure the relative content of fatty acids and fatty acid carbon stable isotope composition data, and to identify the imitation sea cucumber. The method of ginseng origin. Background technique [0002] According to the definition of GT / T 17924-2008, a geographical indication product refers to the use of raw materials produced in a specific region and produced in a specific region according to traditional techniques. The quality, characteristics or reputation are essentially determined by the geographical characteristics of the region of origin. , and products named after the name of the region of origin have been reviewed and approved according to legal procedures. Imitatio...

Claims

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Application Information

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IPC IPC(8): G06F17/50
CPCG06F30/20
Inventor 刘瑀吴鹏赵新达李颖
Owner DALIAN MARITIME UNIVERSITY
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