Ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method

A technology for American ginseng and embryo rescue, applied in the field of plant breeding, can solve problems that have not yet been seen, and achieve the effect of high regeneration frequency

Inactive Publication Date: 2018-11-02
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Except for the above two reports, ther

Method used

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  • Ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method
  • Ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method
  • Ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method

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Experimental program
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Effect test

Embodiment 1

[0049] The method for rescuing immature embryos of the F1 generation of reciprocal crosses of ginseng and American ginseng comprises the following steps:

[0050] Step 1, parental emasculation and pollination: In the early flowering stage of the female parent, select a healthy and disease-free plant, use tweezers to remove all the inner florets, and only leave the outer 2-3 layers of florets that are about to open for emasculation. Immediately after emasculation, dip the pollen of the male parent collected in advance with a clean brush, and gently smear it on the stigma of the female parent. After the pollination is completed, bag it and put it on the record.

[0051] Step 2: Observing and collecting reciprocal cross F1 young species: observe and record the development of young hybrid embryos 7 days, 15 days and 30 days after pollination respectively. After 35 days of pollination, collect immature orthogonal species, and 45 days later, collect immature reciprocal species, wash...

Embodiment 2

[0056] The method for rescuing immature embryos of the F1 generation of reciprocal crosses of ginseng and American ginseng comprises the following steps:

[0057] Step 1, parental emasculation and pollination: In the early flowering stage of the female parent, select a healthy and disease-free plant, use tweezers to remove all the inner florets, and only leave the outer 2-3 layers of florets that are about to open for emasculation. Immediately after emasculation, dip the pollen of the male parent collected in advance with a clean brush, and gently smear it on the stigma of the female parent. After the pollination is completed, bag it and put it on the record.

[0058] Step 2: Observing and collecting reciprocal cross F1 young species: observe and record the development of young hybrid embryos 7 days, 15 days and 30 days after pollination respectively. After 45 days of pollination, collect immature orthogonal species, and 55 days later, collect immature reciprocal species, wash...

Embodiment 3

[0063] The method for rescuing immature embryos of the F1 generation of reciprocal crosses of ginseng and American ginseng comprises the following steps:

[0064] Step 1, parental emasculation and pollination: In the early flowering stage of the female parent, select a healthy and disease-free plant, use tweezers to remove all the inner florets, and only leave the outer 2-3 layers of florets that are about to open for emasculation. Immediately after emasculation, dip the pollen of the male parent collected in advance with a clean brush, and gently smear it on the stigma of the female parent. After the pollination is completed, bag it and put it on the record.

[0065] Step 2: Observing and collecting reciprocal cross F1 young species: observe and record the development of young hybrid embryos 7 days, 15 days and 30 days after pollination respectively. After 40 days of pollination, collect immature orthogonal species, and 50 days later, collect immature reciprocal species, wash...

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Abstract

The invention relates to the technical field of plant breeding, and provides a ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method. The ginseng and American ginseng cis-hybridization and trans-hybridization F1-generation young embryo rescue method comprises the steps of carrying out emasculation in the early stage of blooming of a femaleparent, and carrying out pollination on the female parent by using pollen of a male parent after emasculation is implemented; collecting immature cis-hybridization species and trans-hybridization species 35-45 days later and 45-55 days later separately, and peeling a young embryo for subsequent use; inoculating the young embryo on an induction culture medium and carrying out dark culture to induceformation of a globular embryo; transferring the globular embryo to a new culture medium and cultivating to form a mature cotyledon stage embryo; and transferring the cotyledon stage embryo onto an MS basic culture medium and cultivating until the lower portion of the cotyledon stage embryo begins to root and 2-3 pieces of small leaves grow from the upper portion of the cotyledon stage embryo soas to gradually form a complete plant. When ginseng is the female parent and American ginseng is the male parent, cis-hybridization is implemented; and when the American ginseng is the female parent and the ginseng is the male parent, trans-hybridization is implemented. By the method, the ginseng and American ginseng crossbreeding period can be shortened, and the problem of low planting percent during culture of the young embryo by cis-hybridization and trans-hybridization is solved.

Description

technical field [0001] The invention relates to the technical field of plant breeding, in particular to a method for rescuing immature embryos of the F1 generation of forward and reverse hybridization of ginseng and American ginseng. Background technique [0002] It is an effective means to obtain new materials of species through interspecies hybridization to promote mutual communication between genes. Ginseng and American ginseng are perennial herbal medicinal plants of the Araliaceae family. Although they are similar in shape, they have different effects. Using hybrid breeding technology to create new varieties of traditional Chinese medicines with both excellent traits is the ultimate goal of breeders' continuous efforts. [0003] In traditional ginseng and American ginseng hybrid breeding, hybrid offspring are usually obtained by planting F1 generation hybrids in the field. In recent years, with the development of biological breeding technology, it can be seen that the...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00
CPCA01H1/02A01H4/001A01H4/008
Inventor 雷秀娟王英平宋娟
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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